RESUMO

The goal of this study was to determine the distribution of preantral follicles in bovine ovaries. Follicular distribution in the ovaries (n = 12) was evaluated in the region of the greater curvature of the ovary (GCO) and the region close to the ovarian pedicle (OP) of Bos taurus indicus heifers of the Nelore breed. Two fragments were obtained from each region of the ovary (GCO and OP). The mean weight of the ovaries was 4.04 ± 0.32 g. The mean antral follicle count (AFC) was 54.58 ± 3.55 follicles (minimum and maximum variation of 30 and 71 follicles, respectively). In total, 1123 follicles were visualized in the region of the GCO; 949 (84.5%) of them were primordial follicles and 174 (15.5%) were developing follicles. The region close to the OP contained 1454 follicles, of which 1266 (87%) were primordial follicles and 44 (12.9%) were developing follicles. The OP region showed a higher proportion of intact follicles in the primordial (P < 0.0001) and primary (P = 0.042) stages compared with the GCO region. The proportion of secondary follicles was similar in the OP and GCO regions. The ovaries of two bovine females (16%; 2/12) contained multi-oocytes follicles, which were characterized as primary follicles. Therefore, the distribution of preantral follicles in the bovine ovary was heterogeneous, with the region close to the OP containing a greater number of preantral follicles compared with the GCO region (P < 0.05).

Assuntos

RESUMO

The aim of this study was to evaluate follicular development, morphological integrity, and antioxidant potential of preantral ovarian follicles from Bos taurus indicus females grown in vitro with alpha-lipoic acid. Ovaries (n = 24) of Bos taurus indicus (n = 12) females were collected during slaughter and fragmented. A randomly obtained fragment from each pair of ovaries was fixed in Bouin (non-cultivated control; D0). These fragments were intended for classical histology (morphology and evaluation of follicular growth), and a fragment from each pair of ovaries was frozen at -80°C (non-cultivated control; D0), and assigned for analysis of oxidative stress [thiobarbituric acid reactive substances (TBARS), nitroblue tetrazolium (NBT), and ferric reducing antioxidant power (FRAP)]. The remaining fragments were cultured in vitro for 6 (D6) or 12 (D12) days, containing only minimum essential medium (MEM) or MEM supplemented with alpha-lipoic acid (50, 100, or 250 ng/ml), on an extracellular matrix of agarose gel, in an oven at 38.5ºC. Every 2 days, 100% of the culture medium was replaced. Supplementation with 100 ng/ml was effective for maintaining follicular integrity after 6 days of culture (primordial: 51.28%; development: 36.88%; P < 0.0001). There was no difference (P > 0.05) between treatments compared with the non-cultivated control treatment (D0), using the NBT and TBARS assays. Therefore, supplementation of the in vitro culture medium of bovine preantral ovarian follicles with a concentration of 100 ng/ml of alpha-lipoic acid at 6 days of culture was effective for maintaining follicular integrity and, after 6 days, maintaining stable levels of reactive oxygen species.

Assuntos

RESUMO

We evaluated the effect of quercetin on the in vitro culture of bovine ovarian fragments in relation to morphology, development, and oxidative stress. Ovaries (n = 12) from Nelore heifers (n = 6) were used. Each pair of ovaries was divided into nine fragments, and one fragment from each animal was fixed in Bouin solution for 24 h (histology control) or frozen (- 80°C; control for oxidative stress). Other ovarian fragments (n = 8) were distributed into concentrations of 0, 10, 25, and 50 µg/mL of quercetin added to the culture medium for 5 or 10 d. Data were analyzed by chi-square test or ANOVA followed by Tukey's test (P < 0.05). Treatment with 25 µg/mL quercetin resulted in the highest proportion of total intact follicles for 5 (67.3%) and 10 d (57.1%); the concentration of 25 µg/mL also presented the best proportion of developing follicles for 5 d (68.7%) and 10 d (62.8%). Treatment with 25 µg/mL quercetin resulted in significant ferric reduction for 10 d of culture, but not for 5 d. No difference (P > 0.1) was observed in the production of reactive oxygen species or in the oxidative degradation of lipids between treatments and non-cultivated controls. Treatment with 25 µg/mL quercetin preserved the morphological integrity of the developing follicles for 5 and 10 d of culture, in addition to promoting the best antioxidant potential after 10 d of culture in bovine ovarian fragments.

Assuntos

RESUMO

A ultrassonografia confere uma avaliação minuciosa do trato reprodutivo de equinos, conferindo relevância no exame andrológico para percepção de higidez ou diagnóstico antecipado de afecções. Portanto, objetivou-se descrever e determinar as mensurações e alterações das glândulas anexas de equinos submetidos à orquiectomia. Garanhões da raça Quarto de Milha (n = 5), com idade de 4-6 anos, foram avaliados por meio de palpação e ultrassonografia transretal previamente à orquiectomia e após, com intervalo de 30, 60 e 90 dias do procedimento. As glândulas bulbouretrais, vesículas seminais e ampolas foram palpadas e delimitadas quanto ao tamanho e higidez. Além disso, procedeu-se o escaneamento e mensuração através da ultrassonografia modo-B, por via transretal. A orquiectomia foi realizada de forma independente ao estudo. Para comparação dos dados utilizou-se o teste T-Student e de Variância (ANOVA). A ultrassonografia mostrou-se eficiente para avaliação e mensuração das ampolas dos ductos deferentes, glândulas vesiculares, bulbouretrais e a próstata de equinos. Considerando as consequências da orquiectomia nas estruturas supracitadas, sugere-se que a mesma não promove hipoplasia das glândulas anexas durante o período de 90 dias após cirurgia. No entanto, apenas as ampolas dos ductos deferentes demonstraram mensurações distintas nesse período, com diminuição significativa associada à orquiectomia.(AU)

Ultrasonography provides a thorough assessment of the reproductive tract of horses, giving relevance to the andrological examination for the perception of health or early diagnosis of conditions. Thus, the objective is to describe the assessment and measurement of the attached glands of horses submitted to orchiectomy. Quarter Horses (n = 5), aged 4-6 years, were assessed by palpation and transrectal ultrasound prior to orchiectomy and after 30, 60 and 90 days after the procedure. Bulbourethral glands, seminal vesicles and ampoules were palpated and delimited in terms of size and health. In addition, scanning and measurement was carried out using B-mode ultrasound, linear probe, frequency of 7.5 Hz, by transrectal approach. Orchiectomy was performed independently from the study. To compare the data, was used T-Student and Variance test (ANOVA). The ultrasonography proved to be efficient for evaluating and measuring the ampoules of the vas deferens, vesicular gland, bulbourethral and the prostate of horses. Considering the consequences of orchiectomy on the aforementioned structures, it is suggested that it does not promote hypoplasia of the attached glands during the period of 90 days after surgery. However, only the ampoules of the vas deferens showed different measurements during this period, with a significant decrease associated with orchiectomy.(AU)

Assuntos

RESUMO

A ultrassonografia confere uma avaliação minuciosa do trato reprodutivo de equinos, conferindo relevância no exame andrológico para percepção de higidez ou diagnóstico antecipado de afecções. Portanto, objetivou-se descrever e determinar as mensurações e alterações das glândulas anexas de equinos submetidos à orquiectomia. Garanhões da raça Quarto de Milha (n = 5), com idade de 4-6 anos, foram avaliados por meio de palpação e ultrassonografia transretal previamente à orquiectomia e após, com intervalo de 30, 60 e 90 dias do procedimento. As glândulas bulbouretrais, vesículas seminais e ampolas foram palpadas e delimitadas quanto ao tamanho e higidez. Além disso, procedeu-se o escaneamento e mensuração através da ultrassonografia modo-B, por via transretal. A orquiectomia foi realizada de forma independente ao estudo. Para comparação dos dados utilizou-se o teste T-Student e de Variância (ANOVA). A ultrassonografia mostrou-se eficiente para avaliação e mensuração das ampolas dos ductos deferentes, glândulas vesiculares, bulbouretrais e a próstata de equinos. Considerando as consequências da orquiectomia nas estruturas supracitadas, sugere-se que a mesma não promove hipoplasia das glândulas anexas durante o período de 90 dias após cirurgia. No entanto, apenas as ampolas dos ductos deferentes demonstraram mensurações distintas nesse período, com diminuição significativa associada à orquiectomia.

Ultrasonography provides a thorough assessment of the reproductive tract of horses, giving relevance to the andrological examination for the perception of health or early diagnosis of conditions. Thus, the objective is to describe the assessment and measurement of the attached glands of horses submitted to orchiectomy. Quarter Horses (n = 5), aged 4-6 years, were assessed by palpation and transrectal ultrasound prior to orchiectomy and after 30, 60 and 90 days after the procedure. Bulbourethral glands, seminal vesicles and ampoules were palpated and delimited in terms of size and health. In addition, scanning and measurement was carried out using B-mode ultrasound, linear probe, frequency of 7.5 Hz, by transrectal approach. Orchiectomy was performed independently from the study. To compare the data, was used T-Student and Variance test (ANOVA). The ultrasonography proved to be efficient for evaluating and measuring the ampoules of the vas deferens, vesicular gland, bulbourethral and the prostate of horses. Considering the consequences of orchiectomy on the aforementioned structures, it is suggested that it does not promote hypoplasia of the attached glands during the period of 90 days after surgery. However, only the ampoules of the vas deferens showed different measurements during this period, with a significant decrease associated with orchiectomy.

Assuntos

RESUMO

Objetivou-se com o presente estudo determinar a ocorrência de alterações das glândulas sexuais acessórias de touros de rodeio através da ultrassonografia transretal. Touros mestiços (n = 118) foramavaliados através da palpação e ultrassonografia transretal. As glândulas sexuais acessórias (próstata, ampolas dos ductos deferentes e vesículas seminais) foram palpadas e delimitadas quanto ao tamanho e higidez. Após, tais glândulas foram avaliadas e mensuradas de ponta a ponta com ultrassom (Sonoscape®, China), probe linear, modo-B, com frequência de 7,5 Hz, por via transretal. A ocorrência de alterações das vesículas seminais direita e esquerda foi de 44,91 %. A assimetria vesicular leve obteve a prevalência de 47,17 %, a moderada de 5,66 % e grave cerca de 47,17 %. A medida média da ampola foi de 9 mm. A ocorrência de alterações das ampolas direita e esquerda foi de 6,78 %, nos quais as alterações na próstata foi de 17,8 %. Assim, cerca de 10,17 % dos touros apresentaram redução da sensibilidade da próstata e no que diz respeito a ausência de sensibilidade dessa glândula, cerca de 7,63%. Conclui-se que o exame ultrassonográfico pode auxiliar em melhor acuidade no exame das glândulas sexuais acessórias em touros de rodeio.(AU)

The objective of this study was to determine the occurrence of alterations of the rodeo bull accessory sex glands by transrectal ultrasound. Crossbred bulls (n = 118) were evaluated by palpation and transrectal ultrasound. The accessory sex glands (prostate, ampoules and seminal vesicles) were palpated and delimited for size and higidity. Afterwards, these glands were scanned and measured from end to end with ultrasound (Sonoscape®, China), linear probe, B-mode, with frequency of 7.5 Hz, transrectally. The occurrence of right and left seminal vesicle alterations was 44.91%. Mild vesicularasymmetry was prevalent at 47.17%, moderate at 5.66% and severe at 47.17%. The mean ampoule measurement was 9 mm. Changes in right and left ampoules were 6.78%, in which prostate changes were 17.8%. Besides, about 10.17% of bulls had reduced prostate sensitivity and about 7.63% lack of sensitivity of this gland. It is concluded that the ultrasound examination may help in better accuracy in the examination of the accessory sex glands in rodeo bulls.(AU)

Assuntos

RESUMO

Objetivou-se com o presente estudo determinar a ocorrência de alterações das glândulas sexuais acessórias de touros de rodeio através da ultrassonografia transretal. Touros mestiços (n = 118) foramavaliados através da palpação e ultrassonografia transretal. As glândulas sexuais acessórias (próstata, ampolas dos ductos deferentes e vesículas seminais) foram palpadas e delimitadas quanto ao tamanho e higidez. Após, tais glândulas foram avaliadas e mensuradas de ponta a ponta com ultrassom (Sonoscape®, China), probe linear, modo-B, com frequência de 7,5 Hz, por via transretal. A ocorrência de alterações das vesículas seminais direita e esquerda foi de 44,91 %. A assimetria vesicular leve obteve a prevalência de 47,17 %, a moderada de 5,66 % e grave cerca de 47,17 %. A medida média da ampola foi de 9 mm. A ocorrência de alterações das ampolas direita e esquerda foi de 6,78 %, nos quais as alterações na próstata foi de 17,8 %. Assim, cerca de 10,17 % dos touros apresentaram redução da sensibilidade da próstata e no que diz respeito a ausência de sensibilidade dessa glândula, cerca de 7,63%. Conclui-se que o exame ultrassonográfico pode auxiliar em melhor acuidade no exame das glândulas sexuais acessórias em touros de rodeio.

The objective of this study was to determine the occurrence of alterations of the rodeo bull accessory sex glands by transrectal ultrasound. Crossbred bulls (n = 118) were evaluated by palpation and transrectal ultrasound. The accessory sex glands (prostate, ampoules and seminal vesicles) were palpated and delimited for size and higidity. Afterwards, these glands were scanned and measured from end to end with ultrasound (Sonoscape®, China), linear probe, B-mode, with frequency of 7.5 Hz, transrectally. The occurrence of right and left seminal vesicle alterations was 44.91%. Mild vesicularasymmetry was prevalent at 47.17%, moderate at 5.66% and severe at 47.17%. The mean ampoule measurement was 9 mm. Changes in right and left ampoules were 6.78%, in which prostate changes were 17.8%. Besides, about 10.17% of bulls had reduced prostate sensitivity and about 7.63% lack of sensitivity of this gland. It is concluded that the ultrasound examination may help in better accuracy in the examination of the accessory sex glands in rodeo bulls.

Assuntos

RESUMO

Folliculogenesis is a process of development and maturation of the ovarian follicles, being essential for the maintenance of fertility. In in vivo conditions, 99.9% of the follicles of an ovary do not ovulate and undergo atresia. In order to minimize this loss and to clarify the existing mechanisms, a technique was developed that allows for the in vitro follicular development. The objective of this study was to evaluate the effects of different epidermal growth factor (EGF) concentrations on the in vitro culturing of equine preantral follicles. Ovaries (n = 10) were collected from a local slaughterhouse of mares in seasonal anestrus, washed with 70% alcohol and PBS, and transported. The inner portion of the ovary was divided into 11 fragments of approximately 3 × 3 × 1 mm. A fragment of each ovary was immediately fixed in Bouin (control group). The remaining 10 fragments were individually cultured for 2 and 6 d. The medium was supplemented with different concentrations of EGF (0, 10, 50, 100, and 200 ng/mL). After cultivation, the fragments were processed and classified according to the developmental stage and morphology. In total, 1065 slides containing 6105 tissue sections were evaluated. Within 2 d of culture, there was a higher proportion of intact follicles at the EGF concentrations of 0 and 100 ng/mL (p > 0.05). After 6 d of culture, only the EGF concentration of 100 ng/mL demonstrated a difference when compared to the other treatments (0, 10, 50 and 200 ng/mL of EGF, p > 0.05). There was follicular development after 2 d at all EGF concentrations. Thus, we suggest that EGF promotes follicular survival in equines at a concentration of 100 ng/mL in in vitro cultures of ovarian fragments for 2 d. In addition, we suggest that EGF promotes follicular survival in equines at a concentration of 100 ng/mL in situ cultivation.

Assuntos

RESUMO

O estudo da contagem de folículos antrais (CFA) é difundido na espécie bovina com intuito de obter este como parâmetro para identificação de fêmeas com melhor eficiência reprodutiva. Assim, de acordo com a CFA da fêmea, essa é classificada conforme o número de folículos presentes no par de ovários, resultando em grupos de alta, média ou baixa CFA. Os parâmetros relacionados à CFA para búfalas ainda são desconhecidos. Portanto, o objetivo do presente estudo foi determinar um parâmetro de classificação da CFA para os pares de ovários da espécie bubalina provenientes de abatedouro. Os pares de ovários bubalinos (n=26) foram coletados no abatedouro de São José do Rio Preto e transportados em recipiente térmico a temperatura de 36º C com solução fisiológica 0,9%. Esses foram identificados (esquerdo/direito) e mantidos no banho maria até a contagem folicular conforme visualização macroscópica. Logo, mensurou-se o diâmetro do maior folículo antral presente no ovário, com auxílio da régua Trident®. Folículos antrais ≥ 1 mm (visíveis a olho nu) presentes nos ovários foram contados para obtenção dos grupos de alta contagem (G-Alta), média (G-Média) e baixa (G-Baixa). A análise estatística para comparação entre a CFA de alta, média e baixa foi realizada utilizando o teste de Fisher, com nível de 5% de significância através do programa estatístico Action 3.1 versão do R 3.0.2 (Campinas, SP, Brasil). Para comparação entre a CFA dos ovários direito e esquerdo utilizou-se o teste T através do mesmo programa estatístico supracitado. Os parâmetros obtidos para CFA do G-Alta foi ≥ 19, assim como para G-Média foi 9 ≥ 18 ≤ e o G-Baixa resultou em ≤ 8 folículos, segue respectivamente a quantidade de animais classificados nos grupos: n=7; n=11 e...

The study of antral follicle count (AFC) is widespread in the bovine species with order to get this as a parameter to identify females with better efficiency. Thus, according to the AFC of the female, this is classified as the number of follicles present in pair of ovaries, resulting in groups of high, medium or low AFC. The parameters related to the AFC for buffaloes are still unknown. Therefore, the objective of the present study was to determine a sort parameter of AFC to the ovaries pairs of the buzzard species from slaughter. The pair of ovaries buffaloes (n= 26) were collected in the slaughterhouse of São José do Rio Preto and transported in thermal container temperature of 36°C with 0.9% saline solution. These have been identified (left/right) and kept in the water bath until the follicular count as macroscopic visualization. Soon, measure the diameter of the largest follicle present in ovarian antral, Trident® with the help of the ruler. Antral follicles ≥ 1 mm (visible to the naked eye) present in the ovaries were counted for obtaining high counting groups (G-I), medium (G-average) and low (G-low). Statistical analysis for comparison between the AFC of high, medium and low was performed using the test of Fisher, with 5% level of significance through the Statistical Programme Action 3.1 R version 3.0.2 (Campinas, SP, Brazil). For comparison between the AFC of the right and left ovaries used T test using the same statistical programme referred to above. The parameters obtained for AFC G-high was ≥ 19, as well as for G-9 average ≥ 18 ≤ and the G-Low resulted in ≤ 8 follows follicles respectively the amount of animals classified in groups: n= 7; n= 11 and n= 8. On comparison between the buffaloes with high, medium and low AFC there was no significant difference (p>0.05). In relation to the...

El estudio del recuento de folículos antrales (AFC) está muy extendido en la especie bovina con el fin de obtener esto como un parámetro para identificar a las hembras con mayor eficiencia. Por lo tanto, de acuerdo con la AFC de la hembra, esto se clasifica como el número de folículos presentes en pares de ovarios, lo que resulta en grupos de AFC alto, medio o bajo. Los parámetros relacionados con el AFC para búfalos aún se desconocen. Por lo tanto, el objetivo del presente estudio fue determinar un parámetro de clasificación de AFC para los pares de ovarios de la especie de ratonero de sacrificio. El par de búfalos de los ovarios (n= 26) se recogió en el matadero de São José do Rio Preto y se transportó en una temperatura decontenedor térmico de 36° C con solución salina al 0,9%. Estos se han identificado (izquierda / derecha) y se mantienen en el baño de agua hasta que el recuento folicular se visualiza macroscópicamente. Pronto, mida el diámetro del folículo más grande presente en el antral ovárico, Trident® con la ayuda de la regla. Los folículos antrales ≥ 1 mm (visibles a simple vista) presentes en los ovarios se contaron para obtener grupos de conteo altos (G-I), medio (promedio G) y bajo (G-bajo). El análisis estadístico para la comparación entre la AFC de alta, media y baja se realizó con la prueba de Fisher, con un nivel de significación del 5% a través del Programa Estadístico Acción 3.1 R versión 3.0.2 (Campinas, SP, Brasil). Para la comparación entre el AFC de los ovarios derecho e izquierdo se utilizó la prueba T utilizando el mismo programa estadístico mencionado anteriormente. Los parámetros obtenidos para AFC G-high fueron ≥ 19, así como para G-9 promedio ≥ 18 ≤ y el G-Low resultó ≤ 8 sigue folículos respectivamente la cantidad de animales clasificados en grupos: n= 7; n= 11 yn= 8. En la comparación entre los búfalos con...

Assuntos

RESUMO

O estudo da contagem de folículos antrais (CFA) é difundido na espécie bovina com intuito de obter este como parâmetro para identificação de fêmeas com melhor eficiência reprodutiva. Assim, de acordo com a CFA da fêmea, essa é classificada conforme o número de folículos presentes no par de ovários, resultando em grupos de alta, média ou baixa CFA. Os parâmetros relacionados à CFA para búfalas ainda são desconhecidos. Portanto, o objetivo do presente estudo foi determinar um parâmetro de classificação da CFA para os pares de ovários da espécie bubalina provenientes de abatedouro. Os pares de ovários bubalinos (n=26) foram coletados no abatedouro de São José do Rio Preto e transportados em recipiente térmico a temperatura de 36º C com solução fisiológica 0,9%. Esses foram identificados (esquerdo/direito) e mantidos no banho maria até a contagem folicular conforme visualização macroscópica. Logo, mensurou-se o diâmetro do maior folículo antral presente no ovário, com auxílio da régua Trident®. Folículos antrais ≥ 1 mm (visíveis a olho nu) presentes nos ovários foram contados para obtenção dos grupos de alta contagem (G-Alta), média (G-Média) e baixa (G-Baixa). A análise estatística para comparação entre a CFA de alta, média e baixa foi realizada utilizando o teste de Fisher, com nível de 5% de significância através do programa estatístico Action 3.1 versão do R 3.0.2 (Campinas, SP, Brasil). Para comparação entre a CFA dos ovários direito e esquerdo utilizou-se o teste T através do mesmo programa estatístico supracitado. Os parâmetros obtidos para CFA do G-Alta foi ≥ 19, assim como para G-Média foi 9 ≥ 18 ≤ e o G-Baixa resultou em ≤ 8 folículos, segue respectivamente a quantidade de animais classificados nos grupos: n=7; n=11 e...(AU)

The study of antral follicle count (AFC) is widespread in the bovine species with order to get this as a parameter to identify females with better efficiency. Thus, according to the AFC of the female, this is classified as the number of follicles present in pair of ovaries, resulting in groups of high, medium or low AFC. The parameters related to the AFC for buffaloes are still unknown. Therefore, the objective of the present study was to determine a sort parameter of AFC to the ovaries pairs of the buzzard species from slaughter. The pair of ovaries buffaloes (n= 26) were collected in the slaughterhouse of São José do Rio Preto and transported in thermal container temperature of 36°C with 0.9% saline solution. These have been identified (left/right) and kept in the water bath until the follicular count as macroscopic visualization. Soon, measure the diameter of the largest follicle present in ovarian antral, Trident® with the help of the ruler. Antral follicles ≥ 1 mm (visible to the naked eye) present in the ovaries were counted for obtaining high counting groups (G-I), medium (G-average) and low (G-low). Statistical analysis for comparison between the AFC of high, medium and low was performed using the test of Fisher, with 5% level of significance through the Statistical Programme Action 3.1 R version 3.0.2 (Campinas, SP, Brazil). For comparison between the AFC of the right and left ovaries used T test using the same statistical programme referred to above. The parameters obtained for AFC G-high was ≥ 19, as well as for G-9 average ≥ 18 ≤ and the G-Low resulted in ≤ 8 follows follicles respectively the amount of animals classified in groups: n= 7; n= 11 and n= 8. On comparison between the buffaloes with high, medium and low AFC there was no significant difference (p>0.05). In relation to the...(AU)

El estudio del recuento de folículos antrales (AFC) está muy extendido en la especie bovina con el fin de obtener esto como un parámetro para identificar a las hembras con mayor eficiencia. Por lo tanto, de acuerdo con la AFC de la hembra, esto se clasifica como el número de folículos presentes en pares de ovarios, lo que resulta en grupos de AFC alto, medio o bajo. Los parámetros relacionados con el AFC para búfalos aún se desconocen. Por lo tanto, el objetivo del presente estudio fue determinar un parámetro de clasificación de AFC para los pares de ovarios de la especie de ratonero de sacrificio. El par de búfalos de los ovarios (n= 26) se recogió en el matadero de São José do Rio Preto y se transportó en una temperatura decontenedor térmico de 36° C con solución salina al 0,9%. Estos se han identificado (izquierda / derecha) y se mantienen en el baño de agua hasta que el recuento folicular se visualiza macroscópicamente. Pronto, mida el diámetro del folículo más grande presente en el antral ovárico, Trident® con la ayuda de la regla. Los folículos antrales ≥ 1 mm (visibles a simple vista) presentes en los ovarios se contaron para obtener grupos de conteo altos (G-I), medio (promedio G) y bajo (G-bajo). El análisis estadístico para la comparación entre la AFC de alta, media y baja se realizó con la prueba de Fisher, con un nivel de significación del 5% a través del Programa Estadístico Acción 3.1 R versión 3.0.2 (Campinas, SP, Brasil). Para la comparación entre el AFC de los ovarios derecho e izquierdo se utilizó la prueba T utilizando el mismo programa estadístico mencionado anteriormente. Los parámetros obtenidos para AFC G-high fueron ≥ 19, así como para G-9 promedio ≥ 18 ≤ y el G-Low resultó ≤ 8 sigue folículos respectivamente la cantidad de animales clasificados en grupos: n= 7; n= 11 yn= 8. En la comparación entre los búfalos con...(AU)

Assuntos