RESUMO
In our IVF & related techniques program, clomiphene 50 mg was used from days 2 to 6 of the cycle in 20 patients and 20 cycles. (17 IVF patients and 3 GIFT patients) menotropins were also used in 2 different schemes: a) 2 ampules in alternated days (12 cases) and b) one ampule daily (8 cases). The patients with the daily scheme required lesser total dose of menotropins and developed higher estradiol levels, although this had none statistic significance, and they also developed a greater number of follicles. There were 16 laparoscopies for ovum capture and 53 oocytes were retrieved (30.2% mature) 10 oocytes were from the GIFT patients. 16 oocytes were fertilized and 8 cleaved. There was only one pregnancy from and IVF patient with the alternated scheme that ended with a first trimester abortion.
Assuntos
Clomifeno/administração & dosagem , Menotropinas/administração & dosagem , Indução da Ovulação/métodos , Adulto , Estradiol/sangue , Feminino , Fertilização in vitro , Transferência Intrafalopiana de Gameta , Humanos , Oócitos/crescimento & desenvolvimento , Folículo Ovariano/fisiologia , Zigoto/crescimento & desenvolvimentoRESUMO
Techniques of "In Vitro" fertilization (IFV) and gamete intrafallopian transfer (GIFT) are proving to be successful in the treatment of infertility. We report here in detail the laboratory procedures of sperm processing for these two techniques also in the present work we pointing out the media quality control methods and the incubation time for the maintenance of spermatozoa fertilizing ability.
Assuntos
Fertilização in vitro/métodos , Transferência Intrafalopiana de Gameta/métodos , Sêmen , Manejo de Espécimes/métodos , Feminino , Humanos , Masculino , Preservação Biológica/métodos , Motilidade dos EspermatozoidesRESUMO
In our IVF + ET & GIFT programs we gave 100 mg of clomiphene from cycle days 2 to 6 in 35 cycles of 32 infertile patients. Serum estradiol curves were made establishing 3 types of curves: 1. climbing curve which was subdivided in curve A (6 cycles) with more than 600 pg/mL of estradiol, more than 4 follicles of a mean size of 35 mm; curve B (13 cycles) with an estradiol range from 300 to 600 pg/mL, 2.9 follicles of 24 mm and curve C with less than 300 pg/mL of estradiol, 0.8 follicles of 22 mm. 2. Flat curve with minimum estradiol level and follicle size and 3. Irregular curve with atypic variations that probably reflect oocyte atresia and cyst formation. The progesterone level in the luteal phase was parallel to the estradiol. There was evidence of multiple follicular growth in 20 cycles (57%). Nine laparoscopies for ovum capture were made, collecting a mean of 1.2 oocytes per laparoscopy; they were fertilized in vitro and later transferred 4 embryos and 4 oocytes were transferred to the fallopian tubes. There was at most one mature oocyte per laparoscopy. No pregnancies were achieved. The clomiphene as a single agent, for its wide availability, might be choice for some women that show a good response, which might depend on several factors, but it cannot be considered the ideal single agent for programs that include ovum pick ups.
Assuntos
Clomifeno/uso terapêutico , Folículo Ovariano/efeitos dos fármacos , Adulto , Avaliação de Medicamentos , Estradiol/sangue , Feminino , Fertilização in vitro , Transferência Intrafalopiana de Gameta , Humanos , Hormônio Luteinizante/sangue , Ciclo Menstrual/sangue , Ciclo Menstrual/efeitos dos fármacos , Folículo Ovariano/diagnóstico por imagem , Folículo Ovariano/fisiologia , Progesterona/sangue , Superovulação/efeitos dos fármacos , Superovulação/fisiologia , UltrassonografiaRESUMO
The changes in enzymatic activity of acid phosphatase (AP) have an important role in ovulation, and it seems that there is relationship between ovocyte fertilization by spermatozoon. In this study AP enzymatic activity in follicular fluid of 32 patients of fertile age who underwent laparoscopy or laparotomy, was determined. Ovarian follicles were functioned in all the patients, and bloody follicles were discarded, as well as the ones without ovule. The ovule was morphologically classified according to complex ovocyte-cummulus-crown radiated in three groups: immature, mature or pre-ovulatory and atresic or post-ovulatory. AP levels in follicular fluid, containing immature ovocytes (0.5 +/- 0.3 UBL) were significantly lower, p greater than 0.05, than AP levels for follicles with mature ovocytes (3.7 +/- 1.3) and postmature (4.8 +/- 3.2); there were no significant differences for AP values in these last two groups. These findings show that these AP levels could be used as an indicator of follicular immaturity, and to choose the optimal time for follicular punction for the in vitro fertilization and gamet transference procedures.
Assuntos
Fosfatase Ácida/metabolismo , Líquido Folicular/enzimologia , Oócitos/crescimento & desenvolvimento , Feminino , Humanos , Fatores de TempoRESUMO
Samples of normal human endometrial tissue from eight young women were initiated in tissue culture. Four biopsies were obtained during the first phase of the cycle and four during the secretory phase. The aim of the work was to characterize ultrastructurally the possible changes of the glandular tissue without being dissociated from the stromal component. At eight hours of incubation there was preservation of all cellular components in the tissue from the first phase of the cycle whereas there were minor degenerative changes in the endometrium from the secretory phase but with outstanding preservation of intranuclear canaliculi typical of this phase. After 16 hours minimal changes were seen in proliferative endometrium while severe changes were present in secretory endometrium. The study suggests that whole endometrium in culture can be used to characterize the short time effect of drugs and substances administered during the first phase of the menstrual cycle.