RESUMO

Copper oxide nanoparticles (CuONPs) have been produced on a large scale because they can be applied across various fields, especially in nano-enabled healthcare and agricultural products. However, the increasing use of CuONPs leads to their release and accumulation into the environment. The CuONPs uptaken by seeds and their implications on germination behavior have been reported, but little is known or understood about their impact on photosynthesis in seed tissues. To fill knowledge gaps, this study evaluated the effects of CuONP concentrations (0-300 mg L-1) on the photosynthetic activity of Inga laurina seeds. The microscopy data showed that CuONPs had an average size distribution of 57.5 ± 0.7 nm. Copper ion release and production of reactive oxygen species (ROS) by CuONPs were also evaluated by dialysis and spectroscopy experiments, respectively. CuONPs were not able to intrinsically generate ROS and released a low content of Cu2⁺ ions (4.5%, w/w). Time evolution of chlorophyll fluorescence imaging and laser-induced fluorescence spectroscopy were used to monitor the seeds subjected to nanoparticles during 168 h. The data demonstrate that CuONPs affected the steady-state maximum chlorophyll fluorescence ( F m ' ), the photochemical efficiency of photosystem II ( F v / F m ), and non-photochemical quenching ( NPQ ) of Inga laurina seeds over time. Besides, the NPQ significantly increased at the seed development stage, near the root protrusion stage, probably due to energy dissipation at this germination step. Additionally, the results indicated that CuONPs can change the oscillatory rhythms of energy dissipation of the seeds, disturbing the circadian clock. In conclusion, the results indicate that CuONPs can affect the photosynthetic behavior of I. laurina seeds. These findings open opportunities for using chlorophyll fluorescence as a non-destructive tool to evaluate nanoparticle impact on photosynthetic activity in seed tissues.

Assuntos

RESUMO

Nos sistemas intensivos de produção de suíno uma das afecções mais prevalentes são as artrites, que causam dor aos animais podendo afetar seu desempenho e bem-estar. Deste modo, o diagnóstico precoce torna-se importante para que se dispense aos animais tratamento adequado em tempo hábil. Objetivou-se com este estudo de caso, avaliar a eficiência do uso das imagens termográficas para identificação de artrite, ou de lesões em suínos. Foram utilizados cinco animais com suspeita de artrite nos membros posteriores. A temperatura superficial (TS) dos membros posteriores foi registrada por meio de imagens termográficas utilizando o termovisor da Testo®. Foram calculadas as médias de 10 pontos da TS de cada membro posterior dos suínos, sendo posteriormente comparadas utilizando o teste t-Student, com confiabilidade de 95%. Os valores médios de TS foram menores (P≤0,05) no membro sadio (33,6°C), do que naquele com artrite (35,6°C) evidenciando que, por meio de imagens termográficas, é possível a identificação de processos inflamatórios em suínos.

causing pain to animals and it may affect their performance and welfare. Thus, the early diagnosis becomes important to allow appropriate treatment to animals as soon as possible. The aim of this case study was to evaluate the efficiency of use of thermographic images for identifying arthritis or inflammatory processes in pigs. Five pigs were selected as they were suspected to present arthritis in the rear limbs. The surface temperature (ST) was recorded by average of thermographic images using the thermal visor from Testo®. There were calculated the averages of 10 points on the ST of each rear limb of the pigs and compared afterwards using t-Student test with reliability of 95% in order to verify if there were difference between the ST in sound rear limb and the limb presenting edema. The mean ST values were lower (P≤0.05) in the sound limb (33.6°C) than those relating to the limb with edema (35.6°C), slowing the use of thermographic images can turn possible the identification of inflammatory processes in subclinical stage in swine.

Assuntos

RESUMO

Nos sistemas intensivos de produção de suíno uma das afecções mais prevalentes são as artrites, que causam dor aos animais podendo afetar seu desempenho e bem-estar. Deste modo, o diagnóstico precoce torna-se importante para que se dispense aos animais tratamento adequado em tempo hábil. Objetivou-se com este estudo de caso, avaliar a eficiência do uso das imagens termográficas para identificação de artrite, ou de lesões em suínos. Foram utilizados cinco animais com suspeita de artrite nos membros posteriores. A temperatura superficial (TS) dos membros posteriores foi registrada por meio de imagens termográficas utilizando o termovisor da Testo®. Foram calculadas as médias de 10 pontos da TS de cada membro posterior dos suínos, sendo posteriormente comparadas utilizando o teste t-Student, com confiabilidade de 95%. Os valores médios de TS foram menores (P≤0,05) no membro sadio (33,6°C), do que naquele com artrite (35,6°C) evidenciando que, por meio de imagens termográficas, é possível a identificação de processos inflamatórios em suínos.(AU)

causing pain to animals and it may affect their performance and welfare. Thus, the early diagnosis becomes important to allow appropriate treatment to animals as soon as possible. The aim of this case study was to evaluate the efficiency of use of thermographic images for identifying arthritis or inflammatory processes in pigs. Five pigs were selected as they were suspected to present arthritis in the rear limbs. The surface temperature (ST) was recorded by average of thermographic images using the thermal visor from Testo®. There were calculated the averages of 10 points on the ST of each rear limb of the pigs and compared afterwards using t-Student test with reliability of 95% in order to verify if there were difference between the ST in sound rear limb and the limb presenting edema. The mean ST values were lower (P≤0.05) in the sound limb (33.6°C) than those relating to the limb with edema (35.6°C), slowing the use of thermographic images can turn possible the identification of inflammatory processes in subclinical stage in swine.(AU)

Assuntos