RESUMO
Felid alphaherpesvirus type 1 (FHV-1) is an important cause of respiratory and ocular diseases in cats worldwide. Mice have been widely used to study the pathogenesis of several human and animal viruses, especially herpesviruses. This study aimed to verify whether BALB/c mice are susceptible to FHV-1 infection. The animals were intranasally inoculated with FHV-1 and their clinical signs were observed from 3 days postinfection (dpi). At 10 dpi, the animals were euthanized and the lungs, liver, spleen, and kidneys were collected for histopathological examination and quantitative polymerase chain reaction. The results showed that mice were infected with FHV-1 and reproduced several features of the disease observed in its natural host. Histological lesions and viral DNA were found in all sampled tissues, with a higher frequency of FHV-1 DNA copies detected in the lungs. All mice were seroconverted to FHV-1 at 7 dpi. To our knowledge, this is the first report of experimental infection of BALB/c mice with FHV-1. Our findings demonstrate that this murine model can contribute to understanding of FHV-1 pathogenesis and may be useful for trials against this virus.(AU)
O herpesvírus felino tipo 1 (FHV-1) é um importante agente causador de doença respiratória e ocular em gatos em todo o mundo. Camundongos têm sido amplamente utilizados para estudar a patogenia de diversos vírus humanos e animais, especialmente os herpesvírus. O objetivo deste estudo foi verificar se camundongos BALB/c poderiam ser suscetíveis a infecção pelo FHV-1. Os animais foram inoculados com FHV-1 intranasalmente e sinais clínicos foram observados a partir do dia 3 após a infecção (dpi). Após dez dias da inoculação, os animais foram eutanasiados e os pulmões, fígado, baço e rins foram coletados para exame histopatológico e PCR quantitativo (qPCR). Os resultados mostraram que os camundongos foram infectados com o FHV-1 e reproduziram várias características da doença que são observadas em seu hospedeiro natural. Lesões histológicas e DNA viral foram detectadas em todos os tecidos amostrados, com maior frequência de DNA do FHV-1 nos pulmões. Todos os camundongos soroconverteram para FHV-1 aos 7 dpi. Para nosso conhecimento, este estudo é o primeiro relato de infecção experimental de camundongos BALB/c com FHV-1. Nossos resultados demonstraram que esse camundongo pode contribuir para o entendimento da patogenia do FHV-1 e pode ser um modelo útil para ensaios contra esse vírus.(AU)
Assuntos
Animais , Reação em Cadeia da Polimerase , Di-Hidrotaquisterol , Herpesviridae , InfecçõesRESUMO
Dioctophymosis is caused by Dioctophyme renale, a nematode that usually affects the right kidney of carnivores. The aim of this study was to report on a case of a dog with progressive weight loss and swollen abdomen that was diagnosed as presenting dioctophymosis. The patient underwent surgical treatment through which 34 nematodes were found, of which 18 were female and 16 were male, with a maximum length of 74 centimeters. The parasites were free in the abdominal cavity and inside the right kidney, and had caused peritonitis, free fluid, severe adherences between the abdominal organs and renal perforation. Parasitic diseases with a high number of specimens of this species are uncommon in dogs. The disease progresses with an inflammatory reaction and consequent formation of adherences and granulomatous tissue. This surrounds the eggs that were eliminated in the abdominal cavity by the free parasites. This disease occurs frequently in the city of Pelotas, Rio Grande do Sul, Brazil, where the patient of this report was living. To the best of authors' knowledge, this is the case with the largest number of specimens of D. renale removed from a single dog in vivo.
Assuntos
Dioctophymatoidea , Doenças do Cão , Infecções por Enoplida , Animais , Brasil , Doenças do Cão/diagnóstico , Doenças do Cão/parasitologia , Cães/parasitologia , Infecções por Enoplida/diagnóstico , Infecções por Enoplida/veterinária , Feminino , Rim/parasitologia , MasculinoRESUMO
Background: Malformations are structural or functional abnormalities in the organs and structures present at birth. Theseconditions are rarely described in the newborns of dogs and can lead to their death. Meroanencephaly is a defect of theneural tube closure malformation, a type of anencephaly and results from a failure of closure of the rostral neuropore(neural crest), and consequently the development of the calvary becomes defective. This study aims to characterize theclinical-pathological aspects of neonatal meroanencephaly since brain malformations are rare in newborn dogs.Case: A 2-day-old English Pointer canine was sent for a necropsy. The newborn belonged to a litter of eight puppies, andonly this one had macroscopic cranial alterations. Another puppy that died as a consequence of being trampled by thebitch was also necropsied. The newborn was alive for 48 h until death and presented apathy, crying, sucking reflex andopisthotonus. Macroscopic examination of the baby revealed flattening of the skull, with a slit at the site of bone symphysis fusion, and a slit in the skin of the parietal region, covered by thin, translucent meningeal tissue. The newborn hadno other macroscopic changes. The heads of the two animals were examined by radiography to identify the features ofanencephaly in one of the animals by visualizing skull bone flattening. Upon removing the skin and exposing the cranialcavity, an irregular reddish mass was revealed, that corresponded microscopically to area cerebrovasculosa, composed ofneurons and rudimentary glial tissue, vascular neoformation and, hemorrhage and congestion. The cranial nerves was notpossible to observe. There was disorganization of the brain areas with no limitation of white and gray matter and scarceneurons and also a region similar to the cerebellum, with a molecular layer but without the Purkinje neurons. In the spinal...(AU)
Assuntos
Animais , Cães , Anencefalia/veterinária , Tubo Neural/anormalidades , Crista Neural/anormalidades , Anormalidades Congênitas/veterinária , Animais Recém-NascidosRESUMO
Background: Malformations are structural or functional abnormalities in the organs and structures present at birth. Theseconditions are rarely described in the newborns of dogs and can lead to their death. Meroanencephaly is a defect of theneural tube closure malformation, a type of anencephaly and results from a failure of closure of the rostral neuropore(neural crest), and consequently the development of the calvary becomes defective. This study aims to characterize theclinical-pathological aspects of neonatal meroanencephaly since brain malformations are rare in newborn dogs.Case: A 2-day-old English Pointer canine was sent for a necropsy. The newborn belonged to a litter of eight puppies, andonly this one had macroscopic cranial alterations. Another puppy that died as a consequence of being trampled by thebitch was also necropsied. The newborn was alive for 48 h until death and presented apathy, crying, sucking reflex andopisthotonus. Macroscopic examination of the baby revealed flattening of the skull, with a slit at the site of bone symphysis fusion, and a slit in the skin of the parietal region, covered by thin, translucent meningeal tissue. The newborn hadno other macroscopic changes. The heads of the two animals were examined by radiography to identify the features ofanencephaly in one of the animals by visualizing skull bone flattening. Upon removing the skin and exposing the cranialcavity, an irregular reddish mass was revealed, that corresponded microscopically to area cerebrovasculosa, composed ofneurons and rudimentary glial tissue, vascular neoformation and, hemorrhage and congestion. The cranial nerves was notpossible to observe. There was disorganization of the brain areas with no limitation of white and gray matter and scarceneurons and also a region similar to the cerebellum, with a molecular layer but without the Purkinje neurons. In the spinal...
Assuntos
Animais , Cães , Anencefalia/veterinária , Anormalidades Congênitas/veterinária , Crista Neural/anormalidades , Tubo Neural/anormalidades , Animais Recém-NascidosRESUMO
Dioctophymosis is caused by Dioctophyme renale, a nematode that usually affects the right kidney of carnivores. The aim of this study was to report on a case of a dog with progressive weight loss and swollen abdomen that was diagnosed as presenting dioctophymosis. The patient underwent surgical treatment through which 34 nematodes were found, of which 18 were female and 16 were male, with a maximum length of 74 centimeters. The parasites were free in the abdominal cavity and inside the right kidney, and had caused peritonitis, free fluid, severe adherences between the abdominal organs and renal perforation. Parasitic diseases with a high number of specimens of this species are uncommon in dogs. The disease progresses with an inflammatory reaction and consequent formation of adherences and granulomatous tissue. This surrounds the eggs that were eliminated in the abdominal cavity by the free parasites. This disease occurs frequently in the city of Pelotas, Rio Grande do Sul, Brazil, where the patient of this report was living. To the best of authors knowledge, this is the case with the largest number of specimens of D. renale removed from a single dog in vivo.(AU)
A dioctofimose é causada por Dioctophyme renale, um nematódeo que geralmente afeta o rim direito de carnívoros. Este trabalho objetivou relatar o caso de um cão, com perda de peso progressiva e abaulamento abdominal, diagnosticado com dioctofimose. O paciente foi submetido a tratamento cirúrgico, o que permitiu o achado de 34 nematódeos, sendo 18 fêmeas e 16 machos, com tamanho máximo de 74 centímetros. Os parasitos encontravam-se livres na cavidade abdominal e no interior do rim direito, causando peritonite, formação de líquido livre, intensas aderências entre os órgãos abdominais e uma perfuração renal. Parasitoses com alto número de exemplares dessa espécie são incomuns em cães. Cursam com reação inflamatória, com consequente formação de aderências e tecido granulomatoso, envolvendo os ovos eliminados na cavidade abdominal pelos parasitos livres. A doença é frequente na cidade de Pelotas, Rio Grande do Sul, Brasil, área de origem da paciente afetada. De acordo com a pesquisa realizada, acredita-se que esse é o caso com o maior número de exemplares de D. renale removidos de um cão in vivo.(AU)
Assuntos
Animais , Cães , Cães/parasitologia , Infecções por Ascaridida/complicações , Infecções por Ascaridida/diagnóstico , Rim/parasitologiaRESUMO
Background: The prevalence of intracranial neoplasms in dogs represents 2.1 to 4.0% of the cases. Brain tumors may beprimary or metastatic. The objective of this study was to describe two cases of intra encephalic neoplasia in elderly dogsreceived for necropsy by the Veterinary Oncology Service in the Federal University of Pelotas.Cases: Case 1: A 12-year-old female canine, without breed and medium size, was received for necropsy. The animal hashad behavioral changes. Macroscopic examination of the encephalus revealed asymmetry and congestion. The organs werecollected and fixed in 10% formalin. In the brain cleavage we noticed an extensive brown-gray mass with reddish areas,expansive, moderately demarcated, soft to cut and discrete hydrocephalus. Serial fragments of the brain and fragmentsof the organs were sent for processing. The slides were stained with the hematoxylin and eosin technique for histopathological analysis. At the microscopic examination, cuboidal cells were observed in the encephalus sometimes in acinar arrangements, of extensive and very limited pattern, diagnosed as ependymoma. Case 2: It is a 15-year-old, female Poodledog, with several tumors. During necropsy multiple subcutaneous nodules, mesentery, intestinal serosa, stomach and liverwere noticed. At the cut these were firm and whitish. No macroscopic changes were observed in the other organs. Fragments of organs and brain were collected and a serial section of the encephalus was performed for further processing andhistological analysis. In the histopathological analysis the masses were constituted by proliferation of sometimes roundedcells, elongated, with rounded nuclei and eosinophilic cytoplasm, allowing the diagnosis of mesothelioma. The samecell pattern was observed in other organs. In the frontal cortex of the encephalus there were small foci of cells similar tothose observed in the mesentery, as well as metastatic...(AU)
Assuntos
Animais , Idoso , Cães , Neoplasias Encefálicas/veterinária , Metástase Neoplásica , Mesotelioma/veterinária , Ependimoma/veterináriaRESUMO
Background: The prevalence of intracranial neoplasms in dogs represents 2.1 to 4.0% of the cases. Brain tumors may beprimary or metastatic. The objective of this study was to describe two cases of intra encephalic neoplasia in elderly dogsreceived for necropsy by the Veterinary Oncology Service in the Federal University of Pelotas.Cases: Case 1: A 12-year-old female canine, without breed and medium size, was received for necropsy. The animal hashad behavioral changes. Macroscopic examination of the encephalus revealed asymmetry and congestion. The organs werecollected and fixed in 10% formalin. In the brain cleavage we noticed an extensive brown-gray mass with reddish areas,expansive, moderately demarcated, soft to cut and discrete hydrocephalus. Serial fragments of the brain and fragmentsof the organs were sent for processing. The slides were stained with the hematoxylin and eosin technique for histopathological analysis. At the microscopic examination, cuboidal cells were observed in the encephalus sometimes in acinar arrangements, of extensive and very limited pattern, diagnosed as ependymoma. Case 2: It is a 15-year-old, female Poodledog, with several tumors. During necropsy multiple subcutaneous nodules, mesentery, intestinal serosa, stomach and liverwere noticed. At the cut these were firm and whitish. No macroscopic changes were observed in the other organs. Fragments of organs and brain were collected and a serial section of the encephalus was performed for further processing andhistological analysis. In the histopathological analysis the masses were constituted by proliferation of sometimes roundedcells, elongated, with rounded nuclei and eosinophilic cytoplasm, allowing the diagnosis of mesothelioma. The samecell pattern was observed in other organs. In the frontal cortex of the encephalus there were small foci of cells similar tothose observed in the mesentery, as well as metastatic...
Assuntos
Animais , Idoso , Cães , Ependimoma/veterinária , Mesotelioma/veterinária , Metástase Neoplásica , Neoplasias Encefálicas/veterináriaRESUMO
This paper to describe a case of dysontogenic urethral metaplasia in a one month old mongrel feline who also had bilateral renal dysplasia. Dysontogenic metaplasia in cats are scarce and this change may be associated with renal dysplasia and/or lower urinary tract. The animal had history of abdominal enlargement since birth and dysuria, eliminating urine only dropwise. Due to the poor prognosis we opted for euthanasia. At necropsy was observed enlarged and distended bladder, reduced kidneys and dilated and tortuous ureters. The urethra was thickened, hard to cut, and histologically, was replacing the connective tissue, cartilage and endochondral ossification areas, which features dysontogenic metaplasia. Both kidneys presented primitive appearance featuring dysplasia. Dysontogenic metaplasia in urinary tract feline with renal dysplasia, has not been described.(AU)
Este trabalho tem como objetivo descrever um caso de metaplasia disontogênica uretral em um felino sem raça definida de um mês de idade, que também apresentava displasia renal bilateral. Dados sobre metaplasia disontogênica em felinos são escassos e a alteração pode estar associada a displasia renal e/ou do trato urinário inferior. O animal apresentava histórico de aumento abdominal desde o nascimento e disúria, eliminando a urina apenas por gotejamento. Devido ao prognóstico desfavorável optou-se pela eutanásia. Na necropsia observou-se bexiga aumentada e dilatada, rins diminuídos e ureteres dilatados e tortuosos. A uretra apresentava-se espessada e firme ao corte, e histologicamente, havia substituição ao tecido conjuntivo, áreas de cartilagem e zonas de ossificação endocondral, o que caracteriza metaplasia disontogênica. Ambos os rins apresentavam aspecto primitivo caracterizando a displasia renal. A metaplasia disontogênica no trato urinário de felinos com displasia renal, não tem sido descrita.(AU)
Assuntos
Animais , Gatos , Gatos/anormalidades , Gatos/fisiologia , Metaplasia/veterinária , Nefropatias/diagnóstico , Nefropatias/veterináriaRESUMO
The aim of this study was to investigate the effect of Eugenia uniflora fruit (red type) extract on metabolic status, as well as on neurochemical and behavioral parameters in an animal model of metabolic syndrome induced by a highly palatable diet (HPD). Rats were treated for 150days and divided into 4 experimental groups: standard chow (SC) and water orally, SC and E. uniflora extract (200mg/kg daily, p.o), HPD and water orally, HPD and extract. Our data showed that HPD caused glucose intolerance, increased visceral fat, weight gain, as well as serum glucose, triacylglycerol, total cholesterol and LDL cholesterol; however, E. uniflora prevented these alterations. The extract decreased lipid peroxidation and prevented the reduction of superoxide dismutase and catalase activities in the prefrontal cortex, hippocampus and striatum of animals submitted to HPD. We observed a HPD-induced reduction of thiol content in these cerebral structures. The extract prevented increased acetylcholinesterase activity in the prefrontal cortex caused by HPD and the increase in immobility time observed in the forced swim test. Regarding chemical composition, LC/MS analysis showed the presence of nine anthocyanins as the major compounds. In conclusion, E. uniflora extract showed benefits against metabolic alterations caused by HPD, as well as exhibited antioxidant and antidepressant-like effects.
Assuntos
Antidepressivos/farmacologia , Antioxidantes/farmacologia , Encéfalo/efeitos dos fármacos , Depressão/prevenção & controle , Eugenia/química , Frutas/química , Síndrome Metabólica/prevenção & controle , Extratos Vegetais/farmacologia , Acetilcolinesterase/metabolismo , Adiposidade/efeitos dos fármacos , Animais , Antidepressivos/isolamento & purificação , Antidepressivos/normas , Antioxidantes/isolamento & purificação , Antioxidantes/normas , Comportamento Animal/efeitos dos fármacos , Biomarcadores/sangue , Glicemia/efeitos dos fármacos , Glicemia/metabolismo , Encéfalo/metabolismo , Encéfalo/fisiopatologia , Catalase/metabolismo , Depressão/sangue , Depressão/fisiopatologia , Depressão/psicologia , Dieta Hiperlipídica , Sacarose Alimentar , Modelos Animais de Doenças , Dislipidemias/sangue , Dislipidemias/induzido quimicamente , Dislipidemias/prevenção & controle , Proteínas Ligadas por GPI/metabolismo , Intolerância à Glucose/sangue , Intolerância à Glucose/induzido quimicamente , Intolerância à Glucose/prevenção & controle , Peroxidação de Lipídeos/efeitos dos fármacos , Lipídeos/sangue , Masculino , Síndrome Metabólica/sangue , Síndrome Metabólica/fisiopatologia , Atividade Motora/efeitos dos fármacos , Obesidade/sangue , Obesidade/induzido quimicamente , Obesidade/prevenção & controle , Fitoterapia , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/normas , Plantas Medicinais , Ratos Wistar , Superóxido Dismutase/metabolismo , Fatores de Tempo , Aumento de Peso/efeitos dos fármacosRESUMO
Abstract Dioctophyme renale is a zoonotic nematode that parasites the kidneys of wild and domestic carnivores, and it has been reported frequently in Brazil. The aim here was to register the number of cases of dogs and cats diagnosed with dioctophymosis by necropsy (1981 to 2014) and ultrasound examination (2010 to 2015) in Pelotas-RS. In this context, a survey was conducted on dioctophymosis cases diagnosed at the Veterinary Pathology Laboratory (LPV) and Veterinary Clinical Hospital (HCV) of the Federal University of Pelotas (UFPel), and at a specialist veterinary imaging diagnostics clinic. In total, 95 cases were registered. The high series of the disease in dogs can be related to the presence of a large number of stray and semi-domestic dogs in the city, and also due to the ingestion of intermediate hosts of D. renale parasitized with the infective larvae. Thus, it can be concluded that Pelotas is a city with favorable conditions for the occurrence of dioctophymosis with high rate of disease in recent years.
Resumo Dioctophyme renale é um nematódeo zoonótico que parasita principalmente o rim de carnívoros silvestres e domésticos, e tem sido relatado com frequência no Brasil. Objetivou-se registrar o número de casos de cães e gatos diagnosticados com dioctofimatose por necropsia (1981 a 2014) e exame ultrassonográfico (2010 a 2015) em Pelotas-RS. Nesse contexto, foi realizado um inquérito de casos de dioctofimatose em animais de companhia no Laboratório de Patologia Veterinária (LPV) e no Hospital de Clínicas Veterinária (HCV) da Universidade Federal de Pelotas (UFPel), e em uma clínica veterinária especializada em diagnóstico por imagem, totalizando 95 casos registrados. A elevada casuística da doença em cães pode estar relacionada à presença de um grande número de cães errantes e semi-domiciliados na cidade, e também devido à ingestão dos hospedeiros intermediários de D. renale parasitados com a larva infectante. Assim, conclui-se que Pelotas é uma cidade propícia para a ocorrência de dioctofimatose, com elevada casuística da doença nos últimos anos.