RESUMO
PURPOSE:: To compare the use of a new cyanoacrylate-based surgical glue and suture with sepa-rate points in skin wounds closure. METHODS:: Thirty-six rats were subjected to a 4cm dorsal longitudinal incision. Twelve were sub-jected to simple suture with polyamide 6-0, 12 rats underwent wall synthesis using Dermabond(r) and 12 was performed cutaneous synthesis with N-2-Butyl-Cyanoacrylate. Twelve of each group was euthanized on the seventh postoperative day, their blood was taken to biochemical tests and a layer of skin and subcutaneous tissue surrounding the surgical scar was randomly divided in two segments, to the submission of tension tests and to histological study. RESULTS:: There were no significant difference between groups (p>0.05). In the soft dermis there was more type I collagen production in group I (p<0.05), group II and III was similar re-sults (p>0.05). In the compact dermis, all 3 groups showed similar results (p>0.05). The biomechanical study was similarity between the glue groups (p>0.05) but the group III proved to be different from the others having a higher resistance (p>0.05) . CONCLUSION:: This glue does not cause any inflammation or kidney and hepatic toxicity. Polyamide sutures are more resistant and the glue should be used alone only in less tension are-as.
Assuntos
Cianoacrilatos/uso terapêutico , Técnicas de Sutura , Adesivos Teciduais/uso terapêutico , Cicatrização , Animais , Distribuição Aleatória , Ratos , Fatores de TempoRESUMO
Purpose: To compare the use of a new cyanoacrylate-based surgical glue and suture with sepa-rate points in skin wounds closure. Methods: Thirty-six rats were subjected to a 4cm dorsal longitudinal incision. Twelve were sub-jected to simple suture with polyamide 6-0, 12 rats underwent wall synthesis using Dermabond(r) and 12 was performed cutaneous synthesis with N-2-Butyl-Cyanoacrylate. Twelve of each group was euthanized on the seventh postoperative day, their blood was taken to biochemical tests and a layer of skin and subcutaneous tissue surrounding the surgical scar was randomly divided in two segments, to the submission of tension tests and to histological study. Results: There were no significant difference between groups (p>0.05). In the soft dermis there was more type I collagen production in group I (p 0.05), group II and III was similar re-sults (p>0.05). In the compact dermis, all 3 groups showed similar results (p>0.05). The biomechanical study was similarity between the glue groups (p>0.05) but the group III proved to be different from the others having a higher resistance (p>0.05) . Conclusion: This glue does not cause any inflammation or kidney and hepatic toxicity. Polyamide sutures are more resistant and the glue should be used alone only in less tension are-as.(AU)
Assuntos
Animais , Ratos , Cianoacrilatos/toxicidade , Cianoacrilatos/uso terapêutico , Técnicas de Sutura , CicatrizaçãoRESUMO
Abstract Purpose: To compare the use of a new cyanoacrylate-based surgical glue and suture with sepa-rate points in skin wounds closure. Methods: Thirty-six rats were subjected to a 4cm dorsal longitudinal incision. Twelve were sub-jected to simple suture with polyamide 6-0, 12 rats underwent wall synthesis using Dermabond(r) and 12 was performed cutaneous synthesis with N-2-Butyl-Cyanoacrylate. Twelve of each group was euthanized on the seventh postoperative day, their blood was taken to biochemical tests and a layer of skin and subcutaneous tissue surrounding the surgical scar was randomly divided in two segments, to the submission of tension tests and to histological study. Results: There were no significant difference between groups (p>0.05). In the soft dermis there was more type I collagen production in group I (p<0.05), group II and III was similar re-sults (p>0.05). In the compact dermis, all 3 groups showed similar results (p>0.05). The biomechanical study was similarity between the glue groups (p>0.05) but the group III proved to be different from the others having a higher resistance (p>0.05) . Conclusion: This glue does not cause any inflammation or kidney and hepatic toxicity. Polyamide sutures are more resistant and the glue should be used alone only in less tension are-as.
Assuntos
Animais , Ratos , Adesivos Teciduais/uso terapêutico , Cicatrização , Técnicas de Sutura , Cianoacrilatos/uso terapêutico , Fatores de Tempo , Distribuição AleatóriaRESUMO
OBJETIVOS: comparar a capacidade de selamento apical de quatro cimentos endodônticos. MÉTODOS: quarenta caninos superiores humanos extraídos foram instrumentados 1 mm aquém do ápice anatômico e distribuídos aleatoriamente em quatro grupos (n=10), de acordo com o cimento endodôntico utilizado para a obturação: Endofill, AH Plus, EndoREZ e Epiphany. Os canais radiculares foram obturados pela técnica da condensação lateral com os cimentos e com cones de guta-percha, exceto o grupo do Epiphany, no qual os cones de resina (Resilon) foram utilizados. Os dentes foram imersos em nanquim por sete dias e submetidos ao processo de diafanização e, então, clarificados empregando-se o salicilato de metila. A extensão de penetração via apical do corante foi medida por meio de um microscópio de mensuração em todas as faces do terço apical. RESULTADOS: AH Plus (0,02 mm ± 0,07), Epiphany (0,00 mm ± 0,00) e EndoREZ (0,32 mm ± 0,62) não diferiram estatisticamente entre si (p>0,01). EndoFill apresentou a maior média de penetração do corante (0,83 mm ± 0,73) e diferiu estatisticamente dos demais (p<0,01). CONCLUSÕES: os três cimentos resinosos apresentaram menor microinfiltração que o cimento à base de óxido de zinco e eugenol, porém, promoveram selamento apical semelhante entre si.
Assuntos
Humanos , Cimentos Dentários , Infiltração Dentária , Obturação do Canal Radicular , Técnicas In Vitro , Cimentos de Resina , Materiais Restauradores do Canal RadicularRESUMO
OBJECTIVES: to compare the apical sealing ability of four root canal sealers. MATERIAL AND METHODS: forty extracted human maxillary canines were instrumented 1 mm short of the anatomical apex and randomly assigned to four groups (n=10), according to the root canal sealer used for obturation: Endofill, AH Plus, EndoREZ and Epiphany. Root canals were obturated with gutta-percha points, except for the Epiphany group, in which resin points (Resilon) were used. The teeth were immersed in India ink for seven days and clarified using methyl salicylate. The extent of apical dye penetration was measured with a measuroscope in all aspects of the canal. RESULTS: AH Plus (0.02 mm ± 0.07), Epiphany (0.00 mm ± 0.00) and EndoREZ (0.32 mm ± 0.62) did not differ statistically to each other (p>0.01). EndoFill presented the highest dye penetration mean (0.83 mm ± 0.73) and was statistically different from the other sealers (p<0.01). CONCLUSION: the resin-based root canal sealers presented lesser apical microleakage than the zinc oxide and eugenol based sealer. No statistical differences were observed among resin based sealers.
OBJETIVOS: comparar a capacidade de selamento apical de quatro cimentos endodônticos. MÉTODOS: quarenta caninos superiores humanos extraídos foram instrumentados 1 mm aquém do ápice anatômico e distribuídos aleatoriamente em quatro grupos (n=10), de acordo com o cimento endodôntico utilizado para a obturação: Endofill, AH Plus, EndoREZ e Epiphany. Os canais radiculares foram obturados pela técnica da condensação lateral com os cimentos e com cones de guta-percha, exceto o grupo do Epiphany, no qual os cones de resina (Resilon) foram utilizados. Os dentes foram imersos em nanquim por sete dias e submetidos ao processo de diafanização e, então, clarificados empregando-se o salicilato de metila. A extensão de penetração via apical do corante foi medida por meio de um microscópio de mensuração em todas as faces do terço apical. RESULTADOS: AH Plus (0,02 mm ± 0,07), Epiphany (0,00 mm ± 0,00) e EndoREZ (0,32 mm ± 0,62) não diferiram estatisticamente entre si (p>0,01). EndoFill apresentou a maior média de penetração do corante (0,83 mm ± 0,73) e diferiu estatisticamente dos demais (p<0,01). CONCLUSÕES: os três cimentos resinosos apresentaram menor microinfiltração que o cimento à base de óxido de zinco e eugenol, porém, promoveram selamento apical semelhante entre si.
Assuntos
Humanos , Cimentos Dentários , Infiltração Dentária , Técnicas In Vitro , Obturação do Canal Radicular , Cimentos de Resina , Materiais Restauradores do Canal RadicularRESUMO
OBJECTIVES: To compare the apical sealing ability of four root canal sealers. MATERIAL AND METHODS: Forty extracted human maxillary canines were instrumented 1 mm short of the anatomical apex and randomly assigned to four groups (n=10), according to the root canal sealer used for obturation: Endofill, AH Plus, EndoREZ and Epiphany. Root canals were obturated with gutta-percha points, except for the Epiphany group, in which resin points (Resilon) were used. The teeth were immersed in India ink for seven days and clarified using methyl salicylate. The extent of apical dye penetration was measured with a measuroscope in all aspects of the canal. RESULTS: AH Plus (0.02 mm +/- 0.07), Epiphany (0.00 mm +/- 0.00) and EndoREZ (0.32 mm +/- 0.62) did not differ statistically to each other (p>0.01). EndoFill presented the highest dye penetration mean (0.83 mm +/- 0.73) and was statistically different from the other sealers (p<0.01). CONCLUSION: The resin-based root canal sealers presented lesser apical microleakage than the zinc oxide and eugenol based sealer. No statistical differences were observed among resin based sealers.
RESUMO
OBJECTIVE: The aim of this research was to evaluate, under the scanning electron microscope, the role of different parameters and angles of incidence of the Nd:YAG laser on the morphology of the radicular dentine. BACKGROUND DATA: Irradiation of dentine with Nd:YAG laser removes smear layer and promotes its fusion and recrystallization, thus decreasing permeability. METHODS: Forty-eight human maxillary canines were randomly divided in four groups of 12 teeth each, according to the following parameters of irradiation: 1 Wand 10 Hz; 1 Wand 20 Hz; 3 Wand 10 Hz; 3 Wand 20 Hz. Two teeth of each group were split longitudinally and the laser was applied perpendicularly to the root canal. Specimens were observed under the SEM, and the morphological alterations in dentine were recorded. RESULTS: The increase of power and frequency of the laser produced more structures that characterize the irradiation of dentine by Nd:YAG laser, such as globular formations, melting and glazing, and ebullitions. Such structures were especially frequent when 3 Wand 20 pps were used as parameters. The removal of the smear layer produced root canals with more dentinal tubules visible under the SEM. CONCLUSIONS: The increase in power and frequency of the Nd:YAG laser irradiation produced more visible morphological alterations than the lower settings. The removal of the smear layer increased the number of visible dentinal tubule openings.
Assuntos
Dentina/efeitos da radiação , Lasers , Raiz Dentária/efeitos da radiação , Dentina/ultraestrutura , Relação Dose-Resposta à Radiação , Humanos , Raiz Dentária/ultraestruturaRESUMO
Avaliou-se a influência do pré-alargamento cervical na determinação do instrumento apical inicial em raízes mésio-vestibulares de molares superiores. Foram selecionados 50 primeiros molares superiores com grau de curvatura da raiz mésio-vestibular padronizado entre 10 e 15 graus. Concluída a cirurgia de acesso e remoção do tecido pulpar, determinou-se o comprimento de trabalho a 1mm do ápice. Os dentes foram divididos aleatoriamente em 5 grupos (n=10), de acordo com o tipo de alargamento realizado: Grupo 1: sem alargamento cervical; Grupo 2: brocas Gates-Glidden; Grupo 3: Orifice Opener; Grupo 4; ProTaper; Grupo 5: LA Axxess. Os canais foram explorados com lima do tipo K inserindo-se passivamente a lima 08 no comprimento de trabalho. A seguir, limas de maiores diâmetros foram sucessivamente introduzidas no canal radicular, até se obter a sensação de travamento no comprimento de trabalho. O diâmetro desse instrumento foi registrado, e este foi fixado em posição no canal com cianoacrilato de metila. Secções transversais realizadas no comprimento de trabalho foram observadas ao microscópio eletrônico de varredura e a discrepância entre o menor diâmetro do canal e o diâmetro do instrumento apical inicial foram calculados para cada amostra, por meio de um software. As médias de diferença entre esses diâmetros obtidas em cada grupo foram submetidas à análise estatística. A análise de variância indicou diferença estatisticamente significante entre os grupos (p<0,001). O teste complementar de Tukey evidenciou a maior discrepância para o grupo que não recebeu o pré-alargamento (média: 0,1543 mm ± 0,0216). As brocas LA Axxess promoveram a menor diferença entre o diâmetro anatômico no comprimento de trabalho e o instrumento apical inicial (média: 0,026 mm ± 0,0037). As brocas Gates-Glidden e os instrumentos Orifice Opener foram estatisticamente semelhantes (p>0,05) (média: 0,1167 mm ± 0,0231 e 0,1313 mm ± 0,0344 respectivamente) O pré-alargamento realizado com instrumentos ProTaper evidenciaram a média de 0,0567mm e desvio padrão 0,0354 para os valores de discrepância. Pode-se concluir que o pré-alargamento dos terços cervical e médio permitiu uma melhor determinação do instrumento apical inicial. O grupo no qual foram utilizados instrumentos LA Axxess refletiu com maior fidelidade o diâmetro anatômico no comprimento de trabalho em raízes mésio-vestibulares de primeiros molares.
Assuntos
Instrumentos Odontológicos , Dente Molar/anatomia & histologia , Ápice Dentário , Cavidade Pulpar/anatomia & histologia , Microscopia Eletrônica de Varredura , Interpretação Estatística de Dados , Raiz DentáriaRESUMO
The purpose of this study was to investigate the influence of cervical preflaring on the determination of the first file that binds at working length (WL) in buccal roots of maxillary premolars. Five groups (n=10) were formed at random and, after standard access cavities, the WL was determined 1 mm short from the apex. In group 1, the initial apical file was inserted without preflaring of cervical and middle thirds of the root canals. In groups 2 to 5, the cervical and middle thirds were enlarged with sizes 90 and 110 Gates-Glidden drills, K(3) Orifice Opener instruments, ProTaper instruments and LA Axxess burs, respectively. Canals were sized manually with K-files, starting with No. 08 K-files inserted passively up to the WL. File sizes were increased until a binding sensation was felt at the WL and the size of the instrument was recorded. Transversal sections of the WL regions were examined under scanning electron microscopy and the discrepancies between the canal diameter and first file to bind at the WL were assessed. Significant differences (p<0.001) were found between the groups. The major discrepancy was found without preflaring (mean 157.8 microm). LA Axxess burs produced the smallest discrepancy (mean 0.8 microm). Gates-Glidden drills and K(3) Orifice Opener instruments showed no significant differences (p>0.05) between their results (83.2 microm and 73.6 microm, respectively). The discrepancy for ProTaper instruments was 35.4 microm on average. In conclusion, the instrument binding technique for determination of the anatomical diameter at the WL was not precise. Preflaring of the cervical and middle thirds improved the determination of the anatomical diameter at the WL, and the type of instrument played a major role. Canals preflared with LA Axxess burs showed a more accurate binding of the files to anatomical diameter.
Assuntos
Dente Pré-Molar/anatomia & histologia , Instrumentos Odontológicos , Cavidade Pulpar/anatomia & histologia , Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Ápice Dentário/anatomia & histologia , Análise de Variância , Humanos , Maxila , Microscopia Eletrônica de Varredura , Colo do DenteRESUMO
O objetivo deste estudo foi avaliar a influência do pré-alargamento cervical na determinação do instrumento apical inicial em raízes vestibulares de pré-molares superiores. Foram selecionados 50 primeiros pré-molares superiores apresentando duas raízes. Após a cirurgia de acesso e determinação do comprimento de trabalho 1mm aquém do ápice, os dentes foram divididos aleatoriamente em 5 grupos distintos, de acordo com o tipo de alargamento realizado no terço cervical e médio de cada canal: Grupo 1- sem alargamento cervical; Grupo 2 brocas Gates-Glidden (90, 110); Grupo 3 instrumentos K3 Orifice Openers; Grupo 4 instrumentos ProTaper; Grupo 5 brocas LA Axxess. Os canais foram explorados com uma lima tipo K inserindo-se passivamente uma lima 08 no comprimento de trabalho. Limas de diâmetros maiores foram sucessivamente introduzidas no canal radicular até obter a sensação de travamento no comprimento de trabalho, e o diâmetro desse instrumento foi registrado. As secções transversais realizadas no comprimento de trabalho foram observadas por microscopia eletrônica de varredura e a diferença entre o menor diâmetro do canal e o diâmetro do instrumento apical inicial foi calculada para cada amostra. Houve diferença estatisticamente significante entre os grupos experimentais (p<0,001). A maior discrepância foi revelada pelo grupo em que não realizou-se o pré-alargamento (média: 157.8 µm). As brocas LA Axxess proporcionaram a menor diferença entre o diâmetro anatômico e o instrumento apical inicial (média: 0.8 µm). As brocas Gates-Glidden e os instrumentos Orifice Opener foram estatisticamente semelhantes (média: 83.2 µm e média: 73.6 µm, respectivamente). Os instrumentos ProTaper apresentaram uma média de 35.4 µm para os valores de discrepância. Pode-se concluir que a técnica de determinação do instrumento apical inicial não é precisa. O pré-alargamento dos terços cervical e médio do canal torna mais fiel a determinação do diâmetro anatômico no comprimento de trabalho. O pré-alargamento do canal realizado com brocas LA Axxess evidenciaram maior precisão do travamento das limas no diâmetro anatômico.