RESUMO
In control programs for vectorial transmission of Chagas' disease, conventional microscopic procedures are generally performed to determine baseline levels of infectivity of vectors. Reported here are data using Polymerase Chain Reaction in the detection of Trypanosoma cruzi in Triatoma dimidiata, one of the principal vectors of Chagas' disease in Ecuador. The microscopy and PCR techniques showed a high percentage of vector infection in Pedro Carbo, province of Guayas (Ecuador), with 44.16% and 46.13% positive insects, respectively. This contrasted with the very low Chagas seropositivity recorded (0.5%). Since T. dimidiata was the only vector of the Chagas' disease found in Pedro Carbo and looking at the vector behavior, our data suggest that despite the high T. dimidiata infection, the low Chagas seropositivity detected is closely associated with the epidemiological and ecological context of T. dimidiata in Pedro Carbo.
Assuntos
Doença de Chagas/transmissão , Insetos Vetores/parasitologia , Triatoma/parasitologia , Trypanosoma cruzi/isolamento & purificação , Animais , Anticorpos Antiprotozoários/sangue , Doença de Chagas/epidemiologia , DNA de Protozoário/análise , Vetores de Doenças , Equador/epidemiologia , Fezes/parasitologia , Humanos , Reação em Cadeia da Polimerase/métodos , Sensibilidade e Especificidade , Estudos Soroepidemiológicos , Trypanosoma cruzi/genética , Trypanosoma cruzi/imunologiaRESUMO
A molecular phylogeographic study of Paragonimus mexicanus collected from Guatemala and Ecuador was performed. Genomic DNA was extracted from individual metacercariae, and two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (CO1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (ITS2)) were amplified by the polymerase chain reaction (PCR). Sequences segregated in a phylogenetic tree according to their geographic origins. ITS2 sequences from Ecuador and Guatemala differed at only one site. Pairwise distances among CO1 sequences within a country were always lower than between countries. Nevertheless, genetic distances between countries were less than between geographical forms of P. westermani that have been suggested to be distinct species. This result suggests that populations from Guatemala and Ecuador are genetically differentiated perhaps at the level of subspecies.
Assuntos
DNA de Helmintos/análise , Paragonimus/genética , Animais , Sequência de Bases , Equador , Guatemala , Haplótipos , Larva , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
We have analysed by multilocus enzyme electrophoresis (MLEE) at 21 genetic loci 10 Trypanosoma cruzi stocks isolated from chronic chagasic patients and 3 stocks isolated from Triatoma dimidiata collected in human habitats from the coastal part of Ecuador (all stocks isolated in August-December 1998). Isoenzyme profiles were compared to those of 4 laboratory-cloned stocks representing the major phylogenetic subdivisions of T. cruzi. This parasite's genetic variability in Ecuador proved to be considerable, even in this limited sample, since all main isoenzyme genotypes were recorded. Four stocks from patients were identical at all loci to the reference stock MNcl2 ('major clonet #39'; T. cruzi II) isolated in Chile. The 3 stocks isolated from T. dimidiata were closely related to the formerly described zymodeme I (T. cruzi I). Finally, 3 stocks from chronic chagasic patients (one with an asymptomatic form, 2 with a cardiac-digestive form) were closely related to the formerly described zymodeme III (presently not classified in either T. cruzi I or T. cruzi II). This is the first observation of this category of T. cruzi genotypes in chronic chagasic patients. In the past it was recorded only in acute patients, wild mammals and wild triatomine bugs. The epidemiological implications of these results are discussed.
Assuntos
Doença de Chagas/enzimologia , Isoenzimas/genética , Proteínas de Protozoários/genética , Trypanosoma cruzi/enzimologia , Adulto , Idoso , Animais , Doença de Chagas/genética , Equador , Ensaio de Imunoadsorção Enzimática , Genótipo , Humanos , Pessoa de Meia-Idade , Trypanosoma cruzi/genéticaAssuntos
Acetilcisteína/farmacologia , Doença de Chagas/tratamento farmacológico , Doença de Chagas/imunologia , Glutationa/farmacologia , Animais , Doença de Chagas/sangue , Glutationa/análogos & derivados , Glutationa/sangue , Tolerância Imunológica/efeitos dos fármacos , Ativação Linfocitária/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Baço/imunologia , Subpopulações de Linfócitos T/efeitos dos fármacos , Subpopulações de Linfócitos T/imunologiaRESUMO
An outbreak of delta hepatitis occurred during 1998 among the Waorani of the Amazon basin of Ecuador. Among 58 people identified with jaundice, 79% lived in four of 22 Waorani communities. Serum hepatitis B surface antigen (HBsAg) was found in the sera of 54% of the jaundiced persons, and 14% of asymptomatic persons. Ninety-five percent of 105 asymptomatic Waorani had hepatitis B core (HBc) IgG antibody, versus 98% of 51 with jaundice. These data confirm that hepatitis B virus (HBV) infection is highly endemic among the Waorani. Sixteen of 23 (70%) HBsAg carriers identified at the onset of the epidemic had serologic markers for hepatitis D virus (HDV) infection. All 16 were jaundiced, where as only two of seven (29%) with negative HDV serology were jaundiced (P = .0006). The delta cases clustered in families, 69% were children and most involved superinfection of people chronically infected with HBV. The data suggest that HDV spread rapidly by a horizontal mode of transmission other than by the sexual route.
Assuntos
Surtos de Doenças , Hepatite D/epidemiologia , Vírus Delta da Hepatite/imunologia , Falência Hepática/epidemiologia , Adolescente , Adulto , Criança , Pré-Escolar , Equador/epidemiologia , Etnicidade/estatística & dados numéricos , Feminino , Anticorpos Anti-Hepatite/sangue , Antígenos de Superfície da Hepatite B/sangue , Hepatite D/complicações , Vírus Delta da Hepatite/genética , Humanos , Lactente , Falência Hepática/etiologia , Masculino , Pessoa de Meia-Idade , RNA Viral/sangueRESUMO
We report the distribution of the APOB signal peptide polymorphism in 5 native populations of South America: 2 samples of Mataco and 1 sample each of Pilagá and Toba from the Argentinian Chaco and 1 sample of Ache from the Paraguay forest. A randomly selected subsample of a previously studied sample from the Cayapa of Ecuador (Scacchi et al. 1997) was reanalyzed to investigate probable differences attributable to sampling, laboratory techniques, or interobserver error. The polymorphism observed in the signal peptide region of the APOB gene among native populations of South America exhibits the same range of variation found among geographic continental populations, confirming the high genetic heterogeneity of South Amerindians. Extremes in the allele prevalences were found among the Mataco and Ache, populations not far apart geographically. The small differences in genotype and allele frequencies between the subsample of the Cayapa analyzed here and the original Cayapa sample and between the 2 Mataco samples were not statistically significant and most likely were due to sampling error.
Assuntos
Apolipoproteínas B/genética , Variação Genética/genética , Indígenas Sul-Americanos/genética , Polimorfismo Genético/genética , Sinais Direcionadores de Proteínas/genética , Alelos , Argentina , Viés , Frequência do Gene/genética , Heterogeneidade Genética , Genótipo , Humanos , ParaguaiRESUMO
Estimates of the incidence of pulmonary tuberculosis in developing countries are based on case reporting from local health laboratories or the annual risk of tuberculin skin test conversion. Because these methods are problematic, the authors used a multiple case ascertainment method to estimate the incidence of pulmonary tuberculosis from 1989 to 1993 in a Peruvian shantytown of 34,000 inhabitants. Two methods, face-to-face interview of all local inhabitants and examination of local laboratory smear records, were used for case gathering. The number of missed cases was estimated by capture-recapture analysis. Survey cases with positive smears were matched to age- and sex-matched controls and interviewed about socioeconomic conditions. The average annual incidence per 100,000 population was 364 (95% confidence interval 293-528) by capture-recapture methods. For the city encompassing the shantytown, the Peruvian Ministry of Heath reported an average annual incidence of 134 cases per 100,000 population. The authors conclude that, in Peru, alarming clusters of pulmonary tuberculosis are masked by government reports that pool zones of disparate incidence. Existing estimators of pulmonary tuberculosis incidence based on tuberculin conversion rates may be invalid in such areas. Within these hyperendemic areas, persons suitable for intensive prophylaxis efforts cannot be reliably identified by housing and socioeconomic risk factors.
PIP: A multiple case ascertainment method was used to estimate the incidence of pulmonary tuberculosis in 1989-93 in a shantytown with 34,000 residents near Lima, Peru. Face-to-face interviews with all residents yielded 191 reports of smear-positive tuberculosis diagnoses at shantytown laboratories and 97 diagnoses from out-of-town laboratories during the study period. Local laboratory smear records identified 354 positive smears, confirming the oral reports of 139 residents (73%) who reported diagnoses at local laboratories. The number of missed cases was estimated by capture-recapture analysis. An average annual incidence of 364 pulmonary tuberculosis cases per 100,000 population was calculated. In contrast, an average annual incidence of 134 cases/100,000 was reported by the Peruvian Ministry of Health for the city (South Lima) encompassing the shantytown. For hyperendemic areas such as shantytowns, various household and socioeconomic factors have been proposed as screening tools to identify those at risk of tuberculosis and in need of chemoprophylaxis. Survey cases with positive smears were matched with controls by age and sex and interviewed about socioeconomic conditions. Logistic regression analysis identified three socioeconomic factors that were protective against pulmonary tuberculosis: a longer residence in the shantytown (odds ratio (OR), 0.91/year; 95% confidence interval (CI), 0.82-0.99), a larger number of doors in the home (OR, 0.80/door; 95% CI, 0.70-0.93), and recent consumption of alcohol (OR, 0.61; 95% CI, 0.29-1.01). The positive predictive value of a model comprised of these three factors was below 1%, however. These findings indicate that clusters of tuberculosis cases in areas such as shantytowns may be masked by their proximity to areas of lower incidence in the absence of special case finding efforts.
Assuntos
Surtos de Doenças , Mycobacterium tuberculosis , Classe Social , Tuberculose Pulmonar/epidemiologia , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Peru/epidemiologia , Fatores Socioeconômicos , Conglomerados Espaço-TemporaisRESUMO
DNA extracted from peripheral blood of two Ecuadorian patients showing severe digestive pathology was amplified by the polymerase chain reaction using a Trypanosoma cruzi specific oligonucleotide primers derived from the primary sequence of a cDNA encoding for a 24 kDa excretory/secretory protein. The positive PCR results together with the clinical findings confirmed that both patients had a digestive pathology due to Chagas' disease. This pathology could be more frequent than previously described in the chagasic endemic regions of Andean countries.
Assuntos
Doença de Chagas/complicações , Doenças do Sistema Digestório/etiologia , Idoso , Animais , Anticorpos Antiprotozoários/sangue , Doença de Chagas/diagnóstico , Doença Crônica , DNA de Protozoário/sangue , Doenças do Sistema Digestório/diagnóstico , Equador , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Trypanosoma cruzi/genética , Trypanosoma cruzi/imunologiaRESUMO
DNA extracted from peripheral blood of two Ecuadorian patients showing severe digestive pathology was amplified by the polymerase chain reaction using a Trypanosoma cruzi specific oligonucleotide primers derived from the primary sequence of a cDNA encoding for a 24 kDa excretory/secretory protein. The positive PCR results together with the clinical findings confirmed that both patients had a digestive pathology due to Chagas' disease. This pathology could be more frequent than previously described in the chagasic endemic regions of Andean countries.
DNA obtido do sangue periférico de dois pacientes equatorianos, que apresentavam severa patologia digestiva, foi amplificado pela "polymerase chain reaction" (PCR) utilizando os oligonucleotídoes específicos do Trypanosoma cruzi, derivados de uma seqüência primária de cDNA codificado de 24 kDa proteína excretória/secretória. Os resultados positivos da PCR junto com os achados clínicos confirmam que os dois pacientes tinham uma patologia digestiva de origem chagásica. Esta patologia poderia ser mais freqüente que a descrita previamente nas regiões endêmicas chagásicas das cidades dos Andes.