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1.
Braz J Biol ; 83: e270217, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37075428

RESUMO

Because of their immense economic, wellness, and remedial value, the seeds of palm (Phoenix dactylifera) were selected with synthesized silver nanoparticles (AgNPs) based on their properties for increasing the antibacterial efficacy of medical cotton. This study aimed to be contingent upon the characterisation of raw cotton fabrics treated by AgNPs with date seed extract (DSE) of P. dactylifera both individually and in combination and to investigate their antibacterial activity against various human pathogens. The prepared cotton materials with the synthesized AgNPs and/or DSE were described by both X-ray diffraction (XRD) and scanning electron microscope (SEM). At the same time, gas chromatography-mass spectrometry (GC-MS) and High-performance liquid chromatography (HPLC) were employed to determine the bioactive components in the aqueous date seed extract. The greater antibacterial activity was recorded by cotton treated with DSE and AgNPs mix, in which inhibition zones of all treatments were against Escherichia coli (8 cm), followed by Staphylococcus aureus (2.33-5.87cm) and Bacillus subtilis (2.17-4.63 cm), respectively. Overall, these findings indicate that treated cotton fabrics with synthesised AgNPs and DSE may be widely applied in various potential biological and medical applications, which could enhance environmental sustainability in closed production and consumption.


Assuntos
Nanopartículas Metálicas , Prata , Humanos , Prata/farmacologia , Prata/química , Nanopartículas Metálicas/química , Antibacterianos/farmacologia , Antibacterianos/química , Staphylococcus aureus , Extratos Vegetais/farmacologia , Extratos Vegetais/química
2.
Braz. j. biol ; 83: e270217, 2023. tab, graf, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1429985

RESUMO

Because of their immense economic, wellness, and remedial value, the seeds of palm (Phoenix dactylifera) were selected with synthesized silver nanoparticles (AgNPs) based on their properties for increasing the antibacterial efficacy of medical cotton. This study aimed to be contingent upon the characterisation of raw cotton fabrics treated by AgNPs with date seed extract (DSE) of P. dactylifera both individually and in combination and to investigate their antibacterial activity against various human pathogens. The prepared cotton materials with the synthesized AgNPs and/or DSE were described by both X-ray diffraction (XRD) and scanning electron microscope (SEM). At the same time, gas chromatography-mass spectrometry (GC-MS) and High-performance liquid chromatography (HPLC) were employed to determine the bioactive components in the aqueous date seed extract. The greater antibacterial activity was recorded by cotton treated with DSE and AgNPs mix, in which inhibition zones of all treatments were against Escherichia coli (8 cm), followed by Staphylococcus aureus (2.33-5.87cm) and Bacillus subtilis (2.17-4.63 cm), respectively. Overall, these findings indicate that treated cotton fabrics with synthesised AgNPs and DSE may be widely applied in various potential biological and medical applications, which could enhance environmental sustainability in closed production and consumption.


Devido ao imenso valor econômico, propriedades curativas e de bem-estar, as sementes de palmeira (Phoenix dactylifera) foram selecionadas com nanopartículas de prata sintetizadas (AgNPs), com base em suas propriedades, visando aumentar a eficácia antibacteriana do algodão medicinal. Este estudo teve como objetivo a caracterização de tecidos de algodão cru tratados por AgNPs com extrato de semente de tâmara (DSE) de P. dactylifera tanto individualmente quanto em combinação e investigar sua atividade antibacteriana contra vários patógenos humanos. Os materiais de algodão preparados com os AgNPs sintetizados e/ou DSE foram descritos por difração de raios-X (XRD) e microscópio eletrônico de varredura (SEM). Ao mesmo tempo, cromatografia gasosa-espectrometria de massa (GC-MS) e cromatografia líquida de alta eficiência (HPLC) foram empregadas para determinar os componentes bioativos no extrato aquoso de sementes de tâmaras. A maior atividade antibacteriana foi registrada pelo algodão tratado com mistura de DSE e AgNPs, em que os halos de inibição de todos os tratamentos foram contra Escherichia coli (8 cm), seguido por Staphylococcus aureus (2,33-5,87 cm) e Bacillus subtilis (2,17-4,63 cm), respectivamente. De modo geral, essas descobertas indicam que os tecidos de algodão tratados com AgNPs sintetizados e DSE podem ser amplamente aplicados em várias aplicações biológicas e médicas potenciais, o que pode aumentar a sustentabilidade ambiental na produção e consumo fechados.


Assuntos
Prata , Extratos Vegetais , Gossypium , Nanopartículas , Phoeniceae
3.
Genet Mol Res ; 14(4): 11752-62, 2015 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-26436500

RESUMO

The present study explored the systematic inventory of Echinops L. (Asteraceae) of Saudi Arabia, with special reference to the molecular typing of Echinops abuzinadianus Chaudhary, an endemic species to Saudi Arabia, based on the internal transcribed spacer (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA. A sequence similarity search using BLAST and a phylogenetic analysis of the ITS sequence of E. abuzinadianus revealed a high level of sequence similarity with E. glaberrimus DC. (section Ritropsis). The novel primary sequence and the secondary structure of ITS2 of E. abuzinadianus could potentially be used for molecular genotyping.


Assuntos
Asteraceae/genética , DNA Espaçador Ribossômico/genética , Genótipo , Filogenia , Asteraceae/classificação , Sequência de Bases , Dados de Sequência Molecular , Tipagem Molecular/métodos , Conformação de Ácido Nucleico , Arábia Saudita , Alinhamento de Sequência , Análise de Sequência de DNA , Especificidade da Espécie
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