RESUMO
The morphology of cystacanths and adults of Profilicollis altmani (Perry, 1942) Van Cleave, 1947 (Polymorphidae) were studied from the Pacific mole crab Emerita analoga (Stimpson) (Crustacea, Hippidae) and Belcher's gull Larus belcheri (Vigors) (Aves, Laridae), respectively, in Peru. Comparative morphometrics with accounts of other populations of P. altmani from elsewhere off the Pacific and Atlantic coasts of North and South America revealed marked intraspecific population variations. We report scanning electron micrographs (SEM) of new features, not before noted or captured in line drawings by earlier observers. We further present microscope images that reveal internal details not previously reported or possible to see with SEM. Energy dispersive X-ray analysis (EDXA) revealed unusual patterns in the chemistry of proboscis hooks especially the high sulfur and diminished phosphorous and calcium in hook tips and low sulfur and high levels of phosphorous and calcium at mid hooks. The size and shape of all hooks of the cystacanths are reported for the first time. Histopathological studies in L. belcheri from Peru are also included. Cystacanths of P. altmani from California were also analyzed for molecular patterns and compared with other sequences reported from other locations. The molecular data and the analysis of our new sequences of cytochrome oxidase I (COI) showed that haplotypes of P. altmani had low genetic variation; the species is not geographically structured, and within its clade no monophyletic group is formed.
TITLE: Évaluation comparative de la morphologie de Profilicollis altmani (Acanthocephala, Polymorphidae) de crustacés et d'oiseaux de rivage au Pérou, en particulier l'analyse élémentaire des crochet (EDXA), l'imagerie SEM, l'histopathologie et le profil moléculaire. ABSTRACT: La morphologie des cystacanthes et des adultes de Profilicollis altmani (Perry, 1942) Van Cleave, 1947 (Polymorphidae) a été étudiée, respectivement, à partir du crustacé Emerita analoga (Stimpson) (Crustacea, Hippidae) et du Goéland Siméon Larus belcheri (Vigors) (Aves, Laridae), au Pérou. La morphométrie comparative avec des données d'autres populations de P. altmani d'autres localités au large des côtes du Pacifique et de l'Atlantique de l'Amérique du Nord et du Sud a révélé des variations intraspécifiques marquées des populations. Nous rapportons des nouvelles observations de microscopie électronique à balayage, non notées auparavant ou non capturées dans des dessins au trait par des observateurs antérieurs. Nous présentons en outre des images au microscope qui révèlent des détails internes qui n'ont pas été signalés auparavant ni possibles à voir avec MET. L'analyse aux rayons X à dispersion d'énergie (EDXA) a révélé des modèles inhabituels dans la chimie des crochets de la trompe, en particulier la teneur élevée en soufre et une diminution du phosphore et du calcium dans les pointes des crochets et des niveaux faibles en soufre et en phosphore et en calcium au milieu des crochets. La taille et la forme de tous les crochets des cystacanthes sont décrites pour la première fois. Des études histopathologiques sur L. belcheri du Pérou sont également incluses. Les cystacanthes de P. altmani de Californie ont également été analysés pour les modèles moléculaires et comparés à d'autres séquences rapportées d'autres endroits. Les données moléculaires et l'analyse de nos nouvelles séquences de cytochrome oxydase I (COI) ont montré que les haplotypes de P. altmani avaient une faible variation génétique. L'espèce n'est pas structurée géographiquement, et au sein de son clade aucun groupe monophylétique n'est formé.
Assuntos
Acantocéfalos , Anomuros , Helmintíase Animal , Animais , Aves , Elétrons , Peru , Raios XRESUMO
PURPOSE: The molecular profile of specimens of Mediorhynchus gallinarum (Bhalero, 1937) collected from chickens, Gallus gallus L. in Indonesia was analysed. The aim of this study was to assess the phylogenetic position of species of Mediorhynchus within the order Giganthorhynchida. METHODS: We used one mitochondrial gene (cytochrome oxidase 1) and one nuclear gene (18S ribosomal RNA) to infer phylogenetic relationships of class Archiacanthocephala. RESULTS: The COI and 18S rDNA genes sequences showed that M. gallinarum had low genetic variation and that this species is sister to Mediorhynchus africanus Amin, Evans, Heckmann, El-Naggar, 2013. The phylogenetic relationships of the Class Archiacanthocephala showed that it is not resolved but, however, were mostly congruent using both genes. A review of host-parasite life cycles and geographic distributions of Archiacanthocephala indicates that mainly small mammals and birds are definitive hosts, while termites, cockroaches, and millipedes are intermediate hosts. CONCLUSIONS: While the intermediate hosts have wide geographic distributions, the narrow distribution of the definitive hosts limit the access of archiacanthocephalans to a wider range of prospective hosts. Additional analyses, to increase taxonomic and character sampling will improve the development of a robust phylogeny and provide more stable classification. The results presented here contribute to better understanding of the ecological and evolutionary relationships that allow the host-parasite co-existence within the class Archiacanthocephala.
Assuntos
Acantocéfalos , Galinhas , Acantocéfalos/genética , Animais , DNA Ribossômico/genética , Estágios do Ciclo de Vida , Mamíferos , Filogenia , Estudos Prospectivos , RNA Ribossômico 18S/genéticaRESUMO
The cystacanths of Neoandracantha peruensis n. gen. n. sp. are described from the ghost crab Ocypode gaudichaudii collected from the Pacific coast of Peru. While it is uncommon to describe acanthocephalan taxa from immature stages, the presence of clear-cut distinguishing features separating the present material from its nearest congeneric taxa, and the absence of adults, justifies the erection N. peruensis. The new genus is distinguished by having three separate fields of trunk spines. Specimens of N. peruensis have a slender trunk with two anterior swellings, 3 separate fields of spines on the foretrunk swelling, and no genital spines on the hindtrunk. The proboscis of the new species is heavily armored with 21-22 longitudinal rows of 22 hooks each. Hook no. 14 is more robust ventrally than dorsally. Cystacanths of N. peruensis also have a long tubular hindtrunk and the males have diagonal testes in the midtrunk swelling. Specimens of the closely related Andracantha Schmidt, 1975 have anteriorly enlarged pear-shaped Corynosoma-like trunks, only two fields of anterior trunk spines with occasional genital spines, and bilateral or tandem testes. Proboscides of species of Andracantha have considerably fewer hooks that gradually decrease in size posteriorly. The taxonomic component of this work is amplified by metal analysis of hooks and spines that shows a marked amount of magnesium (Mg) in hooks but not in spines. The highest level of sulfur (S) was found in the outer layer of hooks and anterior spines. The metal footprint of hooks and spines varies in different species of acanthocephalans and has an interspecific diagnostic value.
Assuntos
Acantocéfalos/classificação , Braquiúros/parasitologia , Acantocéfalos/química , Acantocéfalos/crescimento & desenvolvimento , Acantocéfalos/ultraestrutura , Animais , Cálcio/análise , Microanálise por Sonda Eletrônica , Feminino , Magnésio/análise , Masculino , Microscopia Eletrônica de Varredura , Oceano Pacífico , Peru , Fósforo/análise , Espectrometria por Raios X , Enxofre/análiseRESUMO
Specimens of a new species of Rhadinorhynchus Lühe, 1911 are described from the chub mackerel Scomber japonicus (Scombridae) and the Chilean Jack mackerel Trachurus murphyi (Carangidae) (possibly a subspecies of Trachurus symmetricus) from the Pacific Ocean off the Peruvian coast at the Port of Chicama, La Libertad. Specimens of Rhadinorhynchus oligospinosus n. sp. are somewhat small having 11-14 rows of alternating proboscis hooks with 20-22 hooks each with posteriormost hooks in a continuous ring. Ventral hooks are robust with prominent roots but dorsal hooks are slender and shorter with discoid roots. Trunk spines are in two zones separated by a non-spiny region. Anterior trunk spines are in 2-3 complete circles but posterior spines are only ventral and lateral, and do not extend posterior to the level of the posterior end of the proboscis receptacle in both sexes. The new species is closest to Rhadinorhynchus seriolae (Yamaguti, 1963) Golvan, 1969 found in Japanese and Australian waters, but not as close to 19 other species found in the same Pacific waters off Australia, Japan, and Vietnam. In R. seriolae, posterior trunk spines extend well past the receptacle in females, among other diagnostic differences. Proboscis hooks of the new species were analyzed for chemical elements using X-ray in conjunction with EDAX (energy-dispersive analysis for X-ray) software; sulfur had a higher concentration at the edge than the middle of cut hooks.
Assuntos
Acantocéfalos/classificação , Doenças dos Peixes/parasitologia , Helmintíase Animal/parasitologia , Perciformes/parasitologia , Acantocéfalos/anatomia & histologia , Acantocéfalos/química , Acantocéfalos/ultraestrutura , Animais , Cálcio/análise , Microanálise por Sonda Eletrônica/veterinária , Feminino , Masculino , Microscopia Eletrônica de Varredura/veterinária , Oceano Pacífico , Peru , Fósforo/análise , Espectrometria por Raios X/veterinária , Enxofre/análiseRESUMO
Adults of Rhadinorhynchus ornatus Van Cleave, 1918 were collected from the small intestine of skipjack tuna, Katsuwonus pelamis (Linnaeus), in the high seas of the Pacific Ocean off the west coast of South America (new parasite locality record) and described using optical microscopy and a scanning electron microscope (SEM). Our specimens were somewhat comparable to those described from North America and Japan, but had more trunk spines. Definitive differences between the length and thickness of each of the dorsal and ventral proboscis hooks are noted for the first time, with most ventral middle hooks being relatively shorter and more robust than dorsal middle hooks. The SEM documented, for the first time, the different surface topography of the tegument in the proboscis, the neck, and in 3 trunk regions; the presence of microtrichs in the mid- and posterior trunk regions; the elevated base of trunk spines; the circular arrangement of basal proboscis hooks; the different morphology of all dorsal and ventral proboscis hooks and the striations of their surface; the ribbed surface topography of eggs; the elevated slit-like female gonopore; and the rimmed edge of the bursa. The presence of microtrichs on the tegumental surface is further supported by transmission electron microscopy studies. This is the first report of microtrichs in any species of Acanthocephala and the second report of striations in proboscis hooks. The geographical distribution of R. ornatus appears to correspond, at least in part, to that of its epipelagic primary host, K. pelamis, throughout the world in waters ranging in temperature from 14.7 to 30 C.