RESUMO
The purpose of this work was to assess the re-arrangement of RAR-alpha and pml genes in a group of patients with acute promyelocytic leukaemia (APL) in different stages of the disease. Twenty-two patients with APL were studied. Of them, 17 were at the onset and 5 had achieved already complete haematological remission (CR). Evolutive post-remission studies were performed in 8 cases. The cytogenetic analysis was carried out at diagnosis by means of the G-band technique in 14 patients. The molecular study of RAR-alpha and pml genes was made with the Southern method. The common anomaly, t(15;17) was present in 7 of the 9 cases with evaluable metaphases (78%), in one case a 17q+ was the only cytogenetic alteration and another patient had normal karyotype. The molecular study showed re-arrangement of one or both genes involved in the translocation in the 17 patients studied at the onset of the APL. Those patients studied only in CR showed a germinal configuration. Rearrangement bands reappeared in 3 of the 8 patients evaluated along their clinical course. These results are in concordance with previous studies, in which re-arrangement of RAR-alpha and pml genes had been found in APL, despite there are instances in which no cytogenetic anomalies are found; this confirms the interest of molecular studies in the diagnosis and follow-up of APL patients.
Assuntos
Rearranjo Gênico/genética , Leucemia Promielocítica Aguda/genética , Proteínas de Neoplasias , Proteínas Nucleares , Receptores do Ácido Retinoico/genética , Fatores de Transcrição/genética , Feminino , Humanos , Cariotipagem , Masculino , Proteína da Leucemia Promielocítica , Receptor alfa de Ácido Retinoico , Proteínas Supressoras de TumorRESUMO
Oncogenes bcl1 and bcl2 are located in the rupture site of t(11;14) and t(14; 18), respectively. They have been found to rearrange in different B-cell malignancies. A molecular study of oncogenes bcl1 and bcl2 was carried out in 42 patients diagnosed of B-cell chronic lymphoid leukaemia. In 14.3% of the patients the rearrangement affected bcl1, but no differences were found with regard to stage and clinical course between these patients and those without any oncogen rearrangement. In all the patients studied bc12 was in a germinal configuration.
Assuntos
Cromossomos Humanos Par 14/ultraestrutura , Cromossomos Humanos Par 18/ultraestrutura , Rearranjo Gênico , Leucemia Linfocítica Crônica de Células B/genética , Oncogenes , Translocação Genética , Idoso , Idoso de 80 Anos ou mais , Southern Blotting , DNA de Neoplasias/análise , Feminino , Humanos , Imunofenotipagem , Masculino , Pessoa de Meia-IdadeRESUMO
In order to assess the status of their immunologic system, a study was carried out in 38 adults with sickle-cell anaemia. Fifty healthy blood donors were used as control group. Significant decrease of serum albumin (p less than 0.02) and increase of alpha-globulins (p less than 0.01) and gamma-globulins (p less than 0.001) were present in the patients. They showed also significantly decreased percentage of spontaneous rosette-forming lymphocytes (p less than 0.01) and of lymphocytes responding to anti-CD3 monoclonal antibody (p less than 0.05) with respect to the control group. Such relative T-cell decrease in peripheral blood seemingly took place by means of decreasing CD4-positive subpopulations, whose percentage was significantly lower (p less than 0.001) in the patients than in the control subjects. Functional studies showed a significant decrease (p less than 0.001) of the activity of natural cytotoxic cells. None of the patients had antibodies against human immunodeficiency viruses type 1 and type 2, and 60% of them were positive to cytomegalovirus test. No statistical correlation was found between the immunological findings and the presence of antibodies against such virus, neither such alterations correlated with the number of blood units received by the patients.
Assuntos
Agamaglobulinemia/etiologia , Anemia Falciforme/imunologia , Linfopenia/etiologia , Adolescente , Adulto , Anemia Falciforme/complicações , Citotoxicidade Imunológica , Feminino , Humanos , Ativação Linfocitária , Subpopulações de Linfócitos , Masculino , Pessoa de Meia-IdadeAssuntos
Dengue/imunologia , Linfócitos T/imunologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Imunidade Celular , Masculino , Pessoa de Meia-Idade , Formação de Roseta , Linfócitos T/classificaçãoAssuntos
Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Feminino , Doença de Hodgkin , Imunidade Celular , Linfócitos B , Linfócitos TRESUMO
Se investigaron las causas de muerte en 67 pacientes con el diagnostico de leucemia aguda, ingresados en tres centros asistenciales de Ciudad de La Habana. La hemorragia fue la complicacion fatal mas frecuentemente encontrada, observandose solo en el 47,8% de los pacientes y asociada a infecciones en el 22,4%. La trombocitopenia severa fue la responsble de los sangramientos en la mayoria de los enfermos asociandose con disminucion de los factores plasmaticos de la coagulacion en tres pacientes y con coagulacion intravascular diseminada en otros dos casos. Las infecciones ocuparon el segundo lugar como causa de muerte, las mas comunes eran debidas a germenes gramnegativos; se encontro bajo porcentaje de infecciones micoticas. La insuficiencia de organos vitales fue la causa de la muerte en el 14,9% de los enfermos. Es de interes senalar que el 82% de las muertes occurrieron durante la fase de induccion de la remision o sin haber recibido terapeutica especifica
Assuntos
Adolescente , Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Feminino , Leucemia , Infecções Bacterianas , Hemorragia , TrombocitopeniaRESUMO
Se estudiaron 82 casos de pacientes adultos (40 hombres y 42 mujeres) con anemia drepanocitica, que asistieron a la consulta externa del Instituto de Hematologia e Inmunologia en el periodo 1979-1981. En 34 enfermos el examen fisico fue normal. La mayor parte de los enfermos en los quales se encontro un acortamiento de los miembros inferiores, tenian necrosis aseptica de la cabeza del femur. Los dos pacientes con derrame articular estaban asintomaticos y esta alteracion fue un hallazgo fortuito al realizar el examen fisico. En 34 enfermos (41,4%) el estudio radiologico fue normal. En el resto se encontraron una o mas de las siguientes alteraciones: ensanchamiento del canal medular 43,9% adelgazamiento de la cortical 43,9% y necrosis de la cabeza femoral 20,7%