RESUMO
The influence of histaminergic sites in the preoptic-anterior hypothalamic area (POA-AHA) on the basal release of luteinizing hormone (LH) under a continuous regimen of estradiol, progesterone, or both was studied in ovariectomized rats. Different groups of animals were subjected to the following experimental schedule: at day 1, rats received a s.c. silastic implant filled with oil, estradiol, progesterone, or estradiol plus progesterone. Seven days later (day 7), animals were implanted into the POA-AHA with microinjection cannulae. At day 8 and 9, the different groups of rats were microinjected with 1 microliter of saline solution containing 35 nMol of pyrilamine or metiamide, or 20 nMol of alpha-fluoro-methyl-histidine. At day 10, blood samples were taken through a permanent jugular cannulae implanted in situ the day before. LH concentrations were determined in plasma by RIA. Results showed that the increase of LH plasma levels induced by the ovariectomy was inhibited by the estrogen implant, as expected. Treatment of metiamide or alpha-fluoro-methyl-histidine did not affect the pattern of LH secretion. Nevertheless, treatment of metiamide induced a transient increase in the gonadotropin concentrations that extended for two hours (16:00 and 17:00 H). No change in LH plasma levels was observed in rats bearing the progesterone implant. Treatments (pyrilamine, metiamide, or alpha-fluoro-methyl-histidine into the POA-AHA) had no effect. The transient increase in the hormone levels observed in rats treated with pyrilamine in the estrogen-implanted rats was absent in rats bearing the estrogen-progesterone implant.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Estradiol/farmacologia , Histamina/fisiologia , Sistema Hipotálamo-Hipofisário/efeitos dos fármacos , Hipotálamo Anterior/efeitos dos fármacos , Hormônio Luteinizante/metabolismo , Adeno-Hipófise/metabolismo , Progesterona/farmacologia , Receptores Histamínicos/fisiologia , Animais , Interações Medicamentosas , Feminino , Metilistidinas/farmacologia , Metiamida/farmacologia , Ovariectomia , Pirilamina/farmacologia , Ratos , Taxa Secretória/efeitos dos fármacosRESUMO
The participation of histamine (HA) sensitive sites in the preoptic-anterior hypothalamic area (POA-AHA) on prolactin (PRL) and luteinizing hormone (LH) surge induced by estrogen was studied in ovariectomized rats. Different groups of animals were subjected to the following experimental schedule: On day "0" rats were stereotaxically implanted into the POA-AHA with microinjection cannulae. On day "1", rats were injected s.c. with estrogen. On days "2" and "3", animals were microinjected into the POA-AHA with different drugs, according to the type of experiment, and at day "4", through a silastic cannula implanted previously in the jugular vein, blood samples were taken each hour between 15:00-21:00 h. In the plasma, PRL and LH concentrations were measured by RIA. Four experiments were performed. In Experiment 1, animals at 12:00 h were injected into the POA-AHA with pyrilamine maleate (an H 1-histamine antagonist), metiamide (an H2-histamine antagonist) or saline as control. In Experiment 2, rats at 12:00 h were injected into the POA-AHA with alpha-fluormethyl-histidine (an inhibitor of histamine synthesis) or the combined administration of pyrilamine and metiamide. In Experiment 3, rats previously microinjected with the histamine synthesis inhibitor were microinjected with 4-methyl-histamine (an H 2-histamine agonists) or 2-pyridilethyl-amine (an H 1-histamine agonist) and in Experiment 4, rats were microinjected at 09:00 h with metiamide, pyrilamine, fluor-methyl-histidine or saline as control. Results showed that in animals treated with pyrilamine or metiamide at noon the prolactin surge induced by estrogen was affected (inhibited by metiamide and shortened by pyrilamine, Experiment 1) and LH surge slightly affected. Rats that received FMH or the combined administration of the histamine antagonists the prolactin and LH surge were abolished (Experiment 2). Only the treatment of the H 2-histamine agonist was able to reproduce the prolactin increase in rats treated with FMH. Nor the H 1 or H 2-histamine agonists were effective in reproducing the LH surge in these animals (Experiment 3). Animals that received saline at 09:00 h into the POA-AHA, the prolactin and LH surges were abolished. Results confirm that histamine in the POA-AHA is important for the expression of prolactin and LH surge induced by estrogen and suggest that H 1- and H 2-histamine receptors are involved in the complex timing mechanisms of the rostral hypothalamus that control both hormone release in rats.