RESUMO
The aim of this study was to investigate the mechanism of propranolol on the regression of hemangiomas. Propranolol-treated hemangioma tissues were collected and the expression of hypoxia inducible factor-1α (HIF-1α) was examined. We also established HIF-1α overexpression and knockdown hemangioma cells, and determined the effects of HIF-1α on the hemangioma cells proliferation, apoptosis, migration and tube formation. Significantly increased HIF-1α level was found in the hemangioma tissues compared to that in normal vascular tissues, whereas propranolol treatment decreased the HIF-1α level in hemangioma tissues in a time- and dose-dependent manner. Moreover, propranolol treatment significantly decreased cell proliferation, migration and tube formation as well as promoted cell apoptosis in HIF-1α overexpression and knockdown hemangioma cells. Propranolol suppressed the cells proliferation, migration and tube formation of hemangioma cells through HIF-1α dependent mechanisms. HIF-1α could serve as a novel target in the treatment of hemangiomas.
Assuntos
Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Hemangioma/tratamento farmacológico , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Propranolol/uso terapêutico , Vasodilatadores/uso terapêutico , Apoptose/efeitos dos fármacos , Hemangioma/metabolismo , HumanosRESUMO
The aim of this study was to investigate the mechanism of propranolol on the regression of hemangiomas. Propranolol-treated hemangioma tissues were collected and the expression of hypoxia inducible factor-1α (HIF-1α) was examined. We also established HIF-1α overexpression and knockdown hemangioma cells, and determined the effects of HIF-1α on the hemangioma cells proliferation, apoptosis, migration and tube formation. Significantly increased HIF-1α level was found in the hemangioma tissues compared to that in normal vascular tissues, whereas propranolol treatment decreased the HIF-1α level in hemangioma tissues in a time- and dose-dependent manner. Moreover, propranolol treatment significantly decreased cell proliferation, migration and tube formation as well as promoted cell apoptosis in HIF-1α overexpression and knockdown hemangioma cells. Propranolol suppressed the cells proliferation, migration and tube formation of hemangioma cells through HIF-1α dependent mechanisms. HIF-1α could serve as a novel target in the treatment of hemangiomas.
Assuntos
Humanos , Propranolol/uso terapêutico , Vasodilatadores/uso terapêutico , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Hemangioma/tratamento farmacológico , Apoptose/efeitos dos fármacos , Hemangioma/metabolismoRESUMO
The humphead wrasse (Cheilinus undulatus) is a large coral fish that has become threatened due to habitat loss and fishing pressure. We sequenced the mitochondrial genome of C. undulatus, using a normal PCR method. The complete mtDNA sequence encoded 13 protein genes, 22 tRNA genes and 2 rRNA genes. It was found to be 16,613 bp in length and had an overall H-strand base compositions of 27.3 for A, 30.9 for C, 16.8 for G, and 25.0% for T. Compared with the sequences of 8 other members of the family Labridae, gene content, genome organization, and nucleotide compositions were similar. All tRNAs formed a typical clover-leaf structure, except tRNA(Ser) (AGY), and most of the size variations among tRNAs stemmed from variations of length in the arm and loop of TΨ, and loop of DHU.