RESUMO
Pathological studies were carried out on the lungs of guinea pigs intratracheally inoculated with 4.6 x 10(6-8) colony forming units (CFU)/head of Actinobacillus pleuropneumoniae serovar 1. All animals in the highest dose group died within 24 hr post inoculation (hpi) and showed pulmonary lesions being hemorrhagic in nature while all animals in the lowest dose group were killed as scheduled at 11 days post inoculation (dpi) and showed only hyperplasia of peribronchial lymphoid tissues. In the middle dose group, two died within 24 hpi, two died at 9 dpi, and the remaining one was killed at 11 dpi. Two guinea pigs which died at 9 dpi showed fibrinonecrotic pleuropneumonia which is the most characteristic acute pulmonary lesion in swine, and has not yet been reproduced in laboratory animals up to the present time. This suggests that guinea pigs may be a useful laboratory animal for studying the pathogenesis of Actinobacillus pleuropneumoniae infection in swine.
Assuntos
Infecções por Actinobacillus/veterinária , Actinobacillus pleuropneumoniae/isolamento & purificação , Cobaias/microbiologia , Pulmão/patologia , Infecções por Actinobacillus/patologia , Actinobacillus pleuropneumoniae/patogenicidade , Animais , Modelos Animais de Doenças , Pulmão/microbiologia , Suínos , Doenças dos Suínos/microbiologiaRESUMO
Histochemical and lectinhistochemical examinations were carried out on nasal mucosa of pigs with or without respiratory diseases. As the results, both acid and neutral mucins coexisted in nasal mucosa of normal pigs while acid sialomucins were mainly observed in nasal mucosa of pigs infected with Bordetella bronchiseptica and/or Pasteurella multocida. Lectinhistochemistry revealed that the nasal epithelial cells of normal pigs were rich in N-acetylgalactosamine, fucose and N-acetyl-glucosamine residues which showed a tendency to disappear in porcine cytomegalovirus infection and to increase in atrophic rhinitis, respectively.
Assuntos
Infecções por Bordetella/veterinária , Bordetella bronchiseptica , Lectinas/metabolismo , Mucosa Nasal/metabolismo , Infecções por Pasteurella/veterinária , Pasteurella multocida , Rinite Atrófica/veterinária , Doenças dos Suínos/metabolismo , Animais , Infecções por Bordetella/metabolismo , Infecções por Bordetella/microbiologia , Infecções por Citomegalovirus/metabolismo , Infecções por Citomegalovirus/veterinária , Infecções por Citomegalovirus/virologia , Células Epiteliais/metabolismo , Glicoconjugados/metabolismo , Histocitoquímica , Infecções por Pasteurella/metabolismo , Infecções por Pasteurella/microbiologia , Rinite Atrófica/metabolismo , Rinite Atrófica/microbiologia , Suínos , Doenças dos Suínos/microbiologiaRESUMO
Histopathological changes in the dorsal skin of hairless descendants of Mexican hairless dogs (MHDs) exposed to artificial irradiation with high energy dose (180 kJ/m2) of ultraviolet (UV) rays (UVA + B) were investigated. Macroscopically, erythema and edema were observed in the irradiated skin at 1 day after irradiation (DAI), and blister formation occurred except one dog at 2 DAI. Erythema almost disappeared at 5 DAI, and at 6 DAI, the skin recovered to almost normal state. Light microscopically, sunburn cells were observed at 1 DAI. Then intercellular edema and blister formation in the epidermis and dermal edema were evident at 2 and 3 DAI. At 6 DAI, the skin showed almost normal features except for slight epidermal thickening, but melanin granules, which were distributed in almost the whole length of the epidermis before UV irradiation, were detected only in cells which seemed to be melanocytes except one dog. Dihydroxyphenylalanine (DOPA)-positive melanocytes almost disappeared at 1 and 2 DAI, and at 6 DAI, the number of DOPA-positive melanocytes increased over the level before UV irradiation. The ultrastructural features of melanocytes were characterized by vacuolated cytoplasm, decreased melanosomes, irregular-shaped nuclei and shortened dendrites at 1 DAI, and returned to normal at 6 DAI. These findings of melanocytes reflect the severity of the skin injury and support weak suntan reaction in this case. In conclusion, severe form of UV-induced skin injury seen in humans could be reproduced in hairless descendants of MHDs exposed to high energy dose of artificial UVA + B.
Assuntos
Lesões Experimentais por Radiação/patologia , Pele/patologia , Raios Ultravioleta/efeitos adversos , Animais , Vesícula/patologia , Di-Hidroxifenilalanina/metabolismo , Cães , Edema/patologia , Eritema/patologia , Histocitoquímica , Queratinócitos/efeitos da radiação , Queratinócitos/ultraestrutura , Masculino , Melanócitos/metabolismo , Melanócitos/efeitos da radiação , Melanócitos/ultraestrutura , Microscopia Eletrônica , Pele/ultraestrutura , Queimadura Solar/patologiaRESUMO
Histochemical studies on tissue sections showed alterations of lectin-binding reactivities in the epididymis, seminal vesicle and ampulla of Brucella ovis-infected rams. These modifications in the carbohydrate composition of organs participating in maturation, transport, and storage of spermatozoa, could be involved in the impaired fertility observed in this disease.
Assuntos
Brucella/isolamento & purificação , Brucelose/veterinária , Epididimo/microbiologia , Glândulas Seminais/microbiologia , Doenças dos Ovinos/microbiologia , Doenças dos Ovinos/patologia , Ducto Deferente/microbiologia , Animais , Brucelose/diagnóstico , Brucelose/patologia , Carboidratos/análise , Epididimo/química , Histocitoquímica/métodos , Lectinas/análise , Masculino , Glândulas Seminais/química , Ovinos , Doenças dos Ovinos/diagnóstico , Ducto Deferente/químicaRESUMO
Ultrastructural characteristics of the dorsal skin of hairless descendants derived from Mexican hairless dogs (3-month-, 1-year- and 5-year-old) were investigated and compared with those of age-matched beagles. Except that beagles had no melanocytes in epidermis, there was no essential difference in ultrastructural characteristics of epidermis between hairless dogs and beagles. In the Stratum basale of epidermis of hairless dogs, a small number of melanocytes and a few Langerhans cell (LC)-like cells were observed among keratinocytes. Melanocytes contained many melanosomes and a few premelanosomes. LC-like cells were characterized by electron-lucent cytoplasm and lobulated nucleus and lacked melanin granules, tonofilaments and desmosomal attachments. Unlike LC in humans and rodents, these LC-like cells did not contain Birbeck granules. Although age-related ultrastructural changes of dorsal skin were generally not clear both in hairless dogs and in beagles, cells containing melanin granules were not infrequently observed among dermal collagen bundles in 5-year-old hairless dogs.
Assuntos
Hipotricose/patologia , Pele/ultraestrutura , Animais , Núcleo Celular/ultraestrutura , Citoplasma/ultraestrutura , Grânulos Citoplasmáticos/química , Grânulos Citoplasmáticos/ultraestrutura , Cães , Epiderme/ultraestrutura , Feminino , Queratinócitos/ultraestrutura , Masculino , Melaninas/análise , Melanócitos/ultraestrutura , Microscopia EletrônicaRESUMO
The skin of hairless descendants of Mexican hairless dogs was investigated lectin-histochemically. Haired dogs were also used for comparative study. In the epidermis of infant pups, lectin staining revealed marked differences between hairless and haired dogs. Griffonia simplicifolia-I (GS-I) bound moderately to the cytoplasm of the stratum basale of hairless dogs, while no binding to that of haired dogs was observed. Bauhinia purpurea (BPA) showed positive staining for the stratum spinosum and basale of hairless dogs, while it was negative or weakly positive for the cytoplasm of both epidermal layers of haired dogs. Maclura pomifera (MPA) showed moderate to intense staining for the intercellular area of the stratum basale of hairless dogs, while no or weak staining was detected in that of haired dogs. Urex europeus-I (UEA-I) bound weakly to moderately to the cytoplasm and the intercellular area of the stratum spinosum of hairless dogs, whereas no or very weak staining was observed in those of haired dogs. Most of the lectins exhibited significant alterations in the epidermal staining pattern between infants and adults. Significant differences were not observed in adult canine epidermis between hairless and haired dogs. In conclusion, lectin histochemistry in hairless dogs revealed that there were different development steps in the epidermal cells between infants and adults.