RESUMO
To achieve a waste-free clean production, the present study aimed to valorize an underused agroindustrial byproduct (rice bran) by mealworms bioconversion and produce bio-oil from pyrolysis of insect excreta (frass) as bioinsecticide. To reach the first goal, the suitability of rice bran (RB) versus standard diet, wheat bran (WB), was examined by determining feed conversion, growth performance, and nutritional profile of T. molitor larvae. RB diet was an appropriate feed substrate for breeding mealworms, as evidenced by their high survival rates, optimal feed conversion parameters, and its capability to support the growth and life cycle of this insect. Besides, RB did not affect soluble larval protein content but modified crude fat content and fatty acid profile. In order to address the second aim, egested frass from RB and WB were subjected to pyrolysis to obtain bio-oils. The main compound was acetic acid (≈37%) followed by 1,6-anhydro-ß-d-glucopyranose (from 16 to 25%), as measured by GC-MS analysis. Nitrogen-containing chemicals accounted for ≈10%. Frass bio-oils could represent a novel source of bioinsecticides due to their bioeffectiveness in insect pests of economic importance (Plodia interpunctella and Tribolium castaneum) and medical interest (Culex pipiens pipiens). For P. interpunctella adults, frass bio-oils produced insecticidal activity by fumigant and contact exposure whereas for T. castaneum adults, just fumigant. By a miniaturized model that simulates semireal storage conditions, it was seen that, on T. castaneum, frass RB bio-oil generated higher repellent effect than frass WB. Finally, bio-oils proved to have larvicidal activity against Cx. p. pipiens.
Assuntos
Tenebrio , Animais , Pirólise , Óleos de Plantas , Fibras na DietaRESUMO
BACKGROUND: The development of novel and ecofriendly tools plays an important role in insect pest management. Nanoemulsions (NEs) based on essential oils (EOs) offer a safer alternative for human health and the environment. This study aimed to elaborate and evaluate the toxicological effects of NEs containing peppermint or palmarosa EOs combined with ß-cypermethrin (ß-CP) using ultrasound technique. RESULTS: The optimized ratio of active ingredients to surfactant was 1:2. The NEs containing peppermint EO combined with ß-CP (NEs peppermint/ß-CP) were polydisperse with two peaks at 12.77 nm (33.4% intensity) and 299.1 nm (66.6% intensity). However, the NEs containing palmarosa EO combined with ß-CP (NEs palmarosa/ß-CP) were monodisperse with a size of 104.5 nm. Both NEs were transparent and stable for 2 months. The insecticidal effect of NEs was evaluated against Tribolium castaneum and Sitophilus oryzae adults, as well as Culex pipiens pipiens larvae. On all these insects, NEs peppermint/ß-CP enhanced pyrethroid bioactivity from 4.22- to 16-folds while NEs palmarosa/ß-CP, from 3.90- to 10.6-folds. Moreover, both NEs maintained high insecticidal activities against all insects for 2 months, although a slight increase of the particle size was detected. CONCLUSION: The NEs elaborated in this work can be considered as highly promising formulations for the development of new insecticides. © 2023 Society of Chemical Industry.
RESUMO
This paper introduces the lethal, sublethal, and ecotoxic effects of peppermint and palmarosa essential oils (EOs) and their polymeric nanoparticles (PNs). The physicochemical analyses indicated that peppermint PNs were polydisperse (PDI > 0.4) with sizes of 381 nm and loading efficiency (LE) of 70.3%, whereas palmarosa PNs were monodisperse (PDI < 0.25) with sizes of 191 nm and LE of 89.7%. EOs and their PNs were evaluated on the adults of rice weevil (Sitophilus oryzae L.) and cigarette beetle (Lasioderma serricorne F.) and the larvae of Culex pipiens pipiens Say. On S. oryzae and L. serricorne, PNs increased EOs' lethal activity, extended repellent effects for 84 h, and also modified behavioral variables during 24 h. Moreover, EOs and PNs generated toxic effects against C. pipiens pipiens. On the other hand, peppermint and palmarosa EOs and their PNs were not toxic to terrestrial non-target organisms, larvae of mealworm (Tenebrio molitor L.), and nymphs of orange-spotted cockroach (Blaptica dubia S.). In addition, PNs were slightly toxic to aquatic non-target organisms, such as brine shrimp (Artemia salina L.). Therefore, these results show that PNs are a novel and eco-friendly formulation to control insect pests.
Assuntos
Repelentes de Insetos , Inseticidas , Nanopartículas , Óleos Voláteis , Tenebrio , Gorgulhos , Animais , Repelentes de Insetos/farmacologia , Inseticidas/farmacologia , Larva , Óleos Voláteis/químicaRESUMO
Mosquitoes (Diptera: Culicidae) are vectors of important parasites and pathogens causing death, poverty and social disability worldwide. The overuse of synthetic insecticides to control mosquito vectors lead to resistance, adverse environmental effects and high operational costs. Therefore, the development of eco-friendly control tools is an important public health challenge. In this study, two different essential oils (EO) (geranium, Geranium maculatum, and bergamot, Citrus bergamia) loaded polymeric nanoparticle (PN) were elaborated using polyethylene glycol (PEG) and chitosan (Qx) as the polymeric matrix/coating. In addition, the mosquito larvicidal acute and residual activity of the PN was evaluated on Culex pipiens pipiens. The physicochemical characterization of PN revealed that PEG-PN had sizes <255 nm and encapsulation efficiency between 68 and 77%; Qx-PN showed sizes <535 nm and encapsulation efficiency between 22 and 38%. From the toxicological test, it was observed that Qx-PN produced higher acute and residual activity than PEG-PN. Overall, this study highlights that polymer nanoparticles containing essential oil are a promising source of eco-friendly mosquito larvicidal products.