RESUMO
INTRODUCTION: Prostate cancer is a common urology malignant in males, ranking second globally. The disease is especially severe when diagnosed alongside hypertension. MKI67 is an established marker of neoplastic cell proliferation in humans, but the significance of its prognostic value in patients with prostate cancer and hypertension requires further research. METHODS: In this retrospective analysis, we evaluated 296 hypertensive prostate cancer patients between March 2, 2012, and November 1, 2015. We used Cox regression models and prediction analysis to assess overall survival. Furthermore, we created a nomogram and verified its accuracy using a calibration curve. RESULTS: Of all participants, 101 (34.12%) died. Our multi-factor analysis revealed that MKI67 expression was associated with an increased hazard ratio of death (> fivefold) (Hazard Ratio 5.829, 95% CI 3.349-10.138, p value < 0.01) and progression (twofold) (HR 2.059, 95% CI 1.368-3.102, p value < 0.01). Our Lasso analysis model displayed that several factors, including heart failure, smoking, ACS, serum albumin, Gealson score, prognostic nutritional index, MKI67 expression, surgery, and stage were high risks of prostate cancer. To ensure each covariate's contribution to cancer prognosis, we created a Cox model nomogram, which accurately predicted the risk of death (C-statistic of 0.8289) and had a proper calibration plot for risk assessment. CONCLUSION: MKI67 expression predicts poor outcomes for overall mortality in prostate cancer and hypertension patients. Additionally, our cross-validated multivariate score, which includes MKI67, demonstrated accuracy efficacy of predicting prognosis.
RESUMO
ABSTRACT Introduction: There are many forms of physical exercise, and the traditional simple exercise can no longer meet people's increasing needs. With the rapid development of the economy, people's living standards have improved progressively. Exercise and fitness have become one of ways for people to relax and improve their quality of life. Objective: Analyze the effects of aerobic gymnastics on male college students' heart rate variability and physical performance. Methods: 10 male college students volunteered for a 6-week aerobic gym-based protocol. This paper discusses the influence of aerobic exercise on heart rate variability and physical fitness of 10 male college students through aerobic interventions for 6 weeks. Results: The HRV indexes SDNN, RMSSD, TP, HF, and LF/HF significantly differed before and after training. Conclusion: There are significant differences in the physical performance of volunteers such as grip strength, flexion of sitting posture, and sitting posture itself before and after the intervention. It is suggested that aerobic training can improve people's cardiac function and physical fitness. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: Existem muitas formas de exercício físico, e a forma tradicional de exercício simples já não pode satisfazer as necessidades crescentes das pessoas. Com o rápido desenvolvimento da economia, o nível de vida das pessoas tem melhorado progressivamente. O exercício físico e a condicionamento físico tornaram-se uma das formas de as pessoas relaxarem e melhorarem a sua qualidade de vida. Objetivo: Analisar os efeitos da ginástica aeróbica sobre a variação do ritmo cardíaco e desempenho físico dos estudantes universitários masculinos. Métodos: 10 estudantes universitários masculinos foram voluntários de um protocolo baseado em ginástica aeróbica com duração de 6 semanas. Este artigo discute a influência do exercício aeróbico na variabilidade do ritmo cardíaco e na aptidão física de 10 estudantes universitários masculinos através de intervenções aeróbicas por 6 semanas. Resultados: Os índices HRV SDNN, RMSSD, TP, HF e LF/HF foram significativamente diferentes antes e depois do treino. Conclusão: Existem diferenças significativas do desempenho físico dos voluntários como força de preensão, flexão da postura sentada e da própria postura sentada antes e depois da intervenção. Sugere-se que o treino aeróbico pode melhorar a função cardíaca e a condição física das pessoas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: Hay muchas formas de ejercicio físico, y la forma tradicional de ejercicio simple ya no puede satisfacer las necesidades crecientes de la gente. Con el rápido desarrollo de la economía, el nivel de vida de la población ha mejorado progresivamente. El ejercicio físico y el acondicionamiento físico se han convertido en una de las formas de relajarse y mejorar la calidad de vida de las personas. Objetivo: Analizar los efectos de la gimnasia aeróbica sobre la variabilidad de la frecuencia cardíaca y el rendimiento físico de estudiantes universitarios de sexo masculino. Métodos: 10 estudiantes universitarios se ofrecieron como voluntarios para un protocolo de 6 semanas de gimnasia aeróbica. Este artículo analiza la influencia del ejercicio aeróbico en la variabilidad de la frecuencia cardíaca y la aptitud física de 10 estudiantes universitarios masculinos mediante intervenciones aeróbicas durante 6 semanas. Resultados: Los índices de HRV SDNN, RMSSD, TP, HF y LF/HF fueron significativamente diferentes antes y después del entrenamiento. Conclusión: Existen diferencias significativas en el rendimiento físico de los voluntarios como la fuerza de agarre, la flexión de la postura sentada y la propia postura sentada antes y después de la intervención. Se sugiere que el entrenamiento aeróbico puede mejorar la función cardíaca y la aptitud física de las personas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
RESUMO
Brazilian grain production increased more than fourfold from 1980 to 2016. The grain boom was achieved primarily by soybean-corn double cropping and cropland expansion-both show changing spatiotemporal patterns since the 1980s. Here, we quantified the contributions of these two strategies to corn and soybean production in Brazil using municipality-level data from 1980 to 2016. We found the contribution of double cropping to the grain boom steadily increased to 35% and the largest driving force was the increasing demand for grain export. While double cropping dominated the conventional agricultural regions, cropland expansion was still the major strategy in agricultural frontiers such as the Centre-West and Matopiba. The implementation of double cropping offset the equivalent of 76.7 million ha of Brazilian arable land for grain production from 2003 to 2016. Double cropping in Brazil has the potential to help alleviate land burdens in other pantropical countries with increasing global food demand.
RESUMO
In this study, our goal was to clarify the role of proteasomal subunit α-1 (PSMA1) in both the differentiation of preadipocytes and the accumulation of lipids in adipocytes. Preadipocytes from healthy one-day-old calves were collected, isolated, and cultured in vitro. The expression pattern of the PSMA1 gene was explored during the differentiation of bovine preadipocytes firstly. Then, the expression of the PSMA1 gene was inhibited by transfection of a chemically synthesized small interfering RNA (siRNA) before differentiation. After induction of differentiation, the mRNA levels of key regulating genes involved in preadipocyte differentiation and the lipid content of mature adipocytes with and without inhibition of PSMA1 were detected by qRT-PCR and oil red O staining, respectively. The data showed that PSMA1 mRNA was differentially expressed during the differentiation of bovine preadipocytes under normal culture conditions in vitro. The expression level of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT enhancer-binding protein alpha (C/EBPα), and lipoprotein lipase (LPL) were significantly decreased in the transfected PSMA1-siRNA group compared with those in the control group, and the mRNA levels of the preadipocyte factor-1 (Pref-1) were significantly upregulated in the transfected PSMA1-siRNA group compared with those in the control group. In addition, significantly fewer lipid droplets were formed by adipocytes transfected with PSMA1-siRNA than by the negative control group (adipocytes transfected with NC-siRNA). Therefore, PSMA1 plays an important role in differentiation and lipid deposition.(AU)
Assuntos
Animais , Bovinos/fisiologia , Adipócitos/fisiologia , Complexo de Endopeptidases do Proteassoma/efeitos adversosRESUMO
A combined tris(trimethylsilyl)silane and visible-light-promoted intramolecular reductive cyclization protocol for the synthesis of indoles and oxindoles has been developed. This straightforward and efficient method shows tolerance towards a broad spectrum of functional-groups and enables rapid and practical synthesis of functionalized nitrogen-based heterocycles in high yields under additive and metal-free, mild photochemical conditions.
Assuntos
Indóis/síntese química , Processos Fotoquímicos , Silanos/química , Ciclização , Radicais Livres/química , Luz , Modelos Químicos , OxirreduçãoRESUMO
Oxidative stress is implicated in the premature death of dopamine neurons in substantia nigra in Parkinson's disease. The incidence of Parkinson's disease is higher in men than in women, and estrogen may provide neuroprotection against oxidative damage. We examined the protective effects of estrogen on rat nigral death after chronic ozone inhalation. Ozone inhalation produced impaired nigral cell morphology and loss of dopamine neurons in ovariectomized rats. This was counteracted after 60 days of 17beta-estradiol treatment, when blood levels were highest. These results indicate that ozone exposure may be a useful Parkinson's disease model and neuroprotection afforded by 17beta-estradiol is dependent on the high levels achieved after its prolonged administration.
Assuntos
Estradiol/farmacologia , Neurônios/efeitos dos fármacos , Oxidantes Fotoquímicos/efeitos adversos , Estresse Oxidativo/efeitos dos fármacos , Ozônio/efeitos adversos , Substância Negra/citologia , Animais , Western Blotting/métodos , Contagem de Células/métodos , Morte Celular/efeitos dos fármacos , Interações Medicamentosas , Estradiol/sangue , Feminino , Imuno-Histoquímica/métodos , Peroxidação de Lipídeos/efeitos dos fármacos , Ratos , Ratos Wistar , Fatores de Tempo , Tirosina 3-Mono-Oxigenase/metabolismoRESUMO
Translational control directed by the eukaryotic translation initiation factor 2 alpha-subunit (eIF2alpha) kinase GCN2 is important for coordinating gene expression programs in response to nutritional deprivation. The GCN2 stress response, conserved from yeast to mammals, is critical for resistance to nutritional deficiencies and for the control of feeding behaviors in rodents. The mouse protein IMPACT has sequence similarities to the yeast YIH1 protein, an inhibitor of GCN2. YIH1 competes with GCN2 for binding to a positive regulator, GCN1. Here, we present evidence that IMPACT is the functional counterpart of YIH1. Overexpression of IMPACT in yeast lowered both basal and amino acid starvation-induced levels of phosphorylated eIF2alpha, as described for YIH1 (31). Overexpression of IMPACT in mouse embryonic fibroblasts inhibited phosphorylation of eIF2alpha by GCN2 under leucine starvation conditions, abolishing expression of its downstream target genes, ATF4 (CREB-2) and CHOP (GADD153). IMPACT bound to the minimal yeast GCN1 segment required for interaction with yeast GCN2 and YIH1 and to native mouse GCN1. At the protein level, IMPACT was detected mainly in the brain. IMPACT was found to be abundant in the majority of hypothalamic neurons. Scattered neurons expressing this protein at higher levels were detected in other regions such as the hippocampus and piriform cortex. The abundance of IMPACT correlated inversely with phosphorylated eIF2alpha levels in different brain areas. These results suggest that IMPACT ensures constant high levels of translation and low levels of ATF4 and CHOP in specific neuronal cells under amino acid starvation conditions.