RESUMO
Two outbreaks of sheeppox in sheep have occurred in Gansu Province, China. The P32, GPCR, and RPO30 genes were used as markers for differential diagnosis. We confirmed that the outbreaks were caused by sheeppox virus. Sequence and phylogenetic analysis of the P32, GPCR, and RPO30 genes revealed a close relationship between the 2 isolates and Chinese sheeppox viruses. Because ill sheep were imported from Jingyuan, another county of Gansu Province, our results strongly suggest the importance of veterinary surveillance prior to transportation.
Assuntos
Capripoxvirus/genética , Infecções por Poxviridae/veterinária , Doenças dos Ovinos/virologia , Proteínas Virais/genética , Sequência de Aminoácidos , Animais , Capripoxvirus/classificação , Capripoxvirus/isolamento & purificação , China , DNA Viral/genética , Surtos de Doenças , Marcadores Genéticos , Dados de Sequência Molecular , Filogenia , Filogeografia , Alinhamento de Sequência , Ovinos/virologiaRESUMO
φC31 integrase encoded by Streptomyces phage can mediate site-specific recombination between phage and host genomes. The recombination direction is generally considered to be accurately regulated, but the regulatory mechanisms involved are still unclear. Recently, some hyperactive mutants of φC31 integrase that can bypass the regulatory steps have been isolated and extensively studied. A putative coiled-coil region is found to play a critical role in controlling recombination direction. Further analysis led us to the speculation that at least two regions in the N-terminal domain of φC31 integrase are involved in the tetrameric interfaces and that the putative coiled coil interacts with one of the regions to regulate the recombination direction.
Assuntos
Bacteriófagos/enzimologia , Integrases/metabolismo , Recombinação Genética , Sequência de Aminoácidos , Pareamento Cromossômico , Integrases/química , Modelos Genéticos , Dados de Sequência Molecular , Mutação/genética , Multimerização Proteica , Estrutura Terciária de Proteína , Alinhamento de SequênciaRESUMO
Osteoporosis is an important and common complex health problem, particularly in postmenopausal women. It is characterized by a reduction in bone mineral density (BMD) and a deterioration of bone microarchitecture with a consequent increase of fracture risk. The osteoprotegerin (OPG) gene is considered to play an important role in the pathogenesis of osteoporosis. We analyzed SNPs of the OPG gene and associations between these polymorphisms and BMD in 399 Chinese postmenopausal women. BMD was quantified at the lumbar spine (L2-4), femoral neck, and total hip. The g.2264T>C and g.27676A>C SNPs were detected by PCR-RFLP and DNA sequencing methods. A significant association with spine BMD was found for g.27676A>C. The spine BMD value for subjects with genotype AA was significantly higher than those with genotypes GA and AA. No significant association was detected between any of the SNP marker genotypes and the other traits. We conclude that g.27676A>C in the OPG gene affects spine BMD and that the C allele is associated with increased risk for osteoporosis in Chinese postmenopausal women.