RESUMO
In laparoscopic cholecystectomy (LC), the treatment of iatrogenic biliary tract injury has been given much attention. However, most accidental right hepatic artery (RHA) injuries are treated with simple clipping. The reason is that the RHA has difficulty in revascularization, and it is generally considered that RHA injury does not cause serious consequences. However, some studies suggest that some cases of RHA ligation can cause a series of pathological changes correlated to arterial ischemia, such as liver abscess, bile tumor, liver atrophy and anastomotic stenosis. Theoretically, RHA blood flow should be restored when possible, in order to avoid the complications of right hepatic ischemia. The present study involved two patients, including one male and one female patient. Both patients were admitted to the hospital with the diagnosis of chronic cholecystitis and gallbladder stone, and developed ischemia of the right half hepatic after accidental transection of the RHA. Both patients underwent continuous end-end anastomosis of the RHA with 6-0 Prolene suture. After the blood vessel anastomosis, the right half liver quickly recovered to its original bright red. No adverse complications were observed in follow-ups at three and six months after the operation. Laparoscopic repair of the RHA is technically feasible. Reconstruction of the RHA can prevent complications associated with right hepatic ischemia.
Assuntos
Colecistectomia/efeitos adversos , Cálculos Biliares/cirurgia , Artéria Hepática/lesões , Complicações Intraoperatórias/cirurgia , Laparoscopia/métodos , Procedimentos Cirúrgicos Vasculares/métodos , Lesões do Sistema Vascular/etiologia , Feminino , Artéria Hepática/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Lesões do Sistema Vascular/diagnóstico , Lesões do Sistema Vascular/cirurgiaRESUMO
Currently, there is a trend of an increasing number of Plasmodium vivax malaria cases in China that are imported across its Southeast Asia border, especially in the China-Myanmar border area (CMB). To date, little is known about the genetic diversity of P. vivax in this region. In this paper, we report the first genome sequencing of a P. vivax isolate (CMB-1) from a vivax malaria patient in CMB. The sequencing data were aligned onto 96.43% of the P. vivax Salvador I reference strain (Sal I) genome with 7.84-fold coverage as well as onto 98.32% of 14 Sal I chromosomes. Using the de novo assembly approach, we generated 8,541 scaffolds and assembled a total of 27.1 Mb of sequence into CMB-1 scaffolds. Furthermore, we identified all 295 known vir genes, which is the largest subtelomeric multigene family in malaria parasites. These results provide an important foundation for further research on P. vivax population genetics.
Assuntos
DNA de Protozoário/análise , Genoma de Protozoário , Plasmodium vivax/genética , Análise de Sequência de DNA , China/epidemiologia , Malária/epidemiologia , Mianmar/epidemiologia , Plasmodium vivax/isolamento & purificaçãoRESUMO
Currently, there is a trend of an increasing number of Plasmodium vivaxmalaria cases in China that are imported across its Southeast Asia border, especially in the China-Myanmar border area (CMB). To date, little is known about the genetic diversity of P. vivaxin this region. In this paper, we report the first genome sequencing of a P. vivaxisolate (CMB-1) from a vivax malaria patient in CMB. The sequencing data were aligned onto 96.43% of the P. vivaxSalvador I reference strain (Sal I) genome with 7.84-fold coverage as well as onto 98.32% of 14 Sal I chromosomes. Using the de novoassembly approach, we generated 8,541 scaffolds and assembled a total of 27.1 Mb of sequence into CMB-1 scaffolds. Furthermore, we identified all 295 known virgenes, which is the largest subtelomeric multigene family in malaria parasites. These results provide an important foundation for further research onP. vivaxpopulation genetics.
Assuntos
DNA de Protozoário/análise , Genoma de Protozoário , Plasmodium vivax/genética , Análise de Sequência de DNA , China/epidemiologia , Malária/epidemiologia , Mianmar/epidemiologia , Plasmodium vivax/isolamento & purificaçãoRESUMO
La carne es una parte muy importante de Ia dieta humana aportando valiosos nutrientes que son beneficiosos para Ia salud. Sin embargo Ia carne es altamente perecedero, por 10 tanto, por un lado, Ias técnicas como el secado y refrigeración deben ser empleados para mantener sus cualidades, por otro lado, el eficaz y los métodos eficientes deben ser desarrollados para evaluar y clasificar sus cualidades. (AU)