RESUMO

New benz[alpha]anthraquinone antibiotics (brasiliquinones A, B and C) with an ethyl group at C-3 were isolated. The producer was identified as Nocardia brasiliensis. The antibiotics were active against Gram-positive bacteria including Mycobacterium sp., but not active against Gram-negative bacteria or fungi. They were also active against multiple drug-resistant P388/ADR tumor cells.

Assuntos

Antibacterianos/isolamento & purificação , Nocardia/metabolismo , Animais , Antraquinonas/isolamento & purificação , Antibacterianos/química , Antibacterianos/farmacologia , Fermentação , Camundongos , Nocardia/classificação , Células Tumorais Cultivadas

RESUMO

A new 32-membered macrolide antibiotic, brasilinolide A was isolated from the fermentation broth of Nocardia sp. IFM 0406. The producer was identified as Nocardia brasiliensis. The antibiotic was only active against Aspergillus niger, but not active against other fungi including yeasts as well as other filamentous like fungi and bacteria. Brasilinolide A exerted an immunosuppressive activity in the assay system of a mixed lymphocyte reaction (MLR).

Assuntos

Antibacterianos/isolamento & purificação , Macrolídeos/química , Macrolídeos/isolamento & purificação , Nocardia/química , Animais , Antibacterianos/biossíntese , Antibacterianos/química , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Células Cultivadas/efeitos dos fármacos , Meios de Cultura , Fungos/efeitos dos fármacos , Células HeLa/efeitos dos fármacos , Humanos , Macrolídeos/farmacologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Nocardia/classificação