RESUMO
BACKGROUND: Human papillomavirus (HPVs) types 6 and 11, are frequently found in non-malignant anogenital condylomas and laryngeal papillomas. However, in an ongoing epidemiological study in Argentina in which 163 anogenital and aero-respiratory cancer biopsies were analyzed, several cases infected with low-risk HPVs could be detected. METHODS: Tumor samples were analyzed by Southern blot hybridization, using HPVs 6, 11, 16, 18, 30, 31, 33 and 35 as probes, and polymerase chain reaction (PCR) with type-specific primers and probes. RESULTS: Four invasive carcinomas containing HPV 6 or 11 could be detected (2.4%) by Southern blot technique. HPV 6 DNA was found in a penile carcinoma, a groin carcinoma, and in a tonsillar carcinoma. One cervical carcinoma harbored HPV 11 DNA. Using HPVs 6, 11, 16, 18 and 33 anticontamination primers and probes in PCR, no additional high-risk HPV types could be detected in these four cancers. CONCLUSION: This report shows the presence of low-risk HPVs (HPV 6 and 11) associated to malignant tumors in a frequency higher than usually observed. The data raise questions about which are the circumstances which may favor low-risk HPV related oncogenesis.
Assuntos
Neoplasias do Sistema Digestório/complicações , Neoplasias dos Genitais Femininos/complicações , Neoplasias Laríngeas/complicações , Papillomaviridae/classificação , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/complicações , Infecções Tumorais por Vírus/complicações , Southern Blotting , Carcinoma de Células Escamosas/química , Carcinoma de Células Escamosas/complicações , Carcinoma de Células Escamosas/virologia , Condiloma Acuminado/complicações , Condiloma Acuminado/genética , Condiloma Acuminado/virologia , DNA Viral/análise , DNA Viral/genética , Neoplasias do Sistema Digestório/química , Neoplasias do Sistema Digestório/virologia , Feminino , Neoplasias dos Genitais Femininos/química , Neoplasias dos Genitais Femininos/virologia , Virilha , Humanos , Neoplasias Laríngeas/química , Neoplasias Laríngeas/virologia , Masculino , Papiloma/química , Papiloma/complicações , Papiloma/virologia , Papillomaviridae/genética , Infecções por Papillomavirus/diagnóstico , Neoplasias Penianas/química , Neoplasias Penianas/complicações , Neoplasias Penianas/virologia , Reação em Cadeia da Polimerase , Infecções Tumorais por Vírus/diagnósticoAssuntos
Humanos , Feminino , Seguimentos , Papillomaviridae/patogenicidade , Infecções por Papillomavirus/complicações , Neoplasias do Colo do Útero/etiologia , Carcinoma de Células Escamosas/etiologia , Colo do Útero/lesões , Infecções por Papillomavirus/diagnóstico , Risco , Neoplasias do Colo do Útero/virologiaRESUMO
Human Papillomaviruses (HPV) are the cause of benign human anogenital lesions where HPV 6 and HPV 11 are most commonly found. Conversely, HPV 16, 18, 31 and 33 are frequently detected in genital carcinomas and are thus considered as oncogenic types. In order to analyze the prevalence of specific HPV types in an Argentine male population, 43 anogenital lesions from different patients with diagnosis of condyloma acuminata were analyzed. These lesions were localized in different regions of the male genitalia comprising the corona glandis, urethral meatus, skin of the penis, scrotum and anus. The biopsies were screened for the presence of HPV 6, 11, 16, 18, 30, 31 and 33 by Southern blot at different stringent conditions of hybridization (Tm -48 degrees C and Tm -20 degrees C). HPV DNA was found in 41 examined cases (95.3) with a clear prevalence of HPV 6 and HPV 11 types (51.2 and 23.3 respectively). Six samples (14.0) were positive only under nonstringent conditions of hybridization. Mixed infections between HPV 16, 18, 30, 31, 33 or a HPV 30 related type with HPV 6 or HPV 11 were detected in 8 specimens (18.6). Only one case was between HPV 16 and HPV 30. Two additional samples were only positive for HPV 30. Experiments in progress about the prevalence of HPV types in female lesions as well as in normal subjects will contribute to complete the description of the epidemiology of these infections in Argentina.
Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Condiloma Acuminado , DNA Viral , Doenças dos Genitais Masculinos/microbiologia , Infecções Tumorais por Vírus/microbiologia , PapillomaviridaeRESUMO
Human Papillomaviruses (HPV) are the cause of benign human anogenital lesions where HPV 6 and HPV 11 are most commonly found. Conversely, HPV 16, 18, 31 and 33 are frequently detected in genital carcinomas and are thus considered as oncogenic types. In order to analyze the prevalence of specific HPV types in an Argentine male population, 43 anogenital lesions from different patients with diagnosis of condyloma acuminata were analyzed. These lesions were localized in different regions of the male genitalia comprising the corona glandis, urethral meatus, skin of the penis, scrotum and anus. The biopsies were screened for the presence of HPV 6, 11, 16, 18, 30, 31 and 33 by Southern blot at different stringent conditions of hybridization (Tm -48 degrees C and Tm -20 degrees C). HPV DNA was found in 41 examined cases (95.3) with a clear prevalence of HPV 6 and HPV 11 types (51.2 and 23.3 respectively). Six samples (14.0) were positive only under nonstringent conditions of hybridization. Mixed infections between HPV 16, 18, 30, 31, 33 or a HPV 30 related type with HPV 6 or HPV 11 were detected in 8 specimens (18.6). Only one case was between HPV 16 and HPV 30. Two additional samples were only positive for HPV 30. Experiments in progress about the prevalence of HPV types in female lesions as well as in normal subjects will contribute to complete the description of the epidemiology of these infections in Argentina.(Au)
Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Idoso , RESEARCH SUPPORT, NON-U.S. GOVT , Condiloma Acuminado/microbiologia , DNA Viral/análise , Doenças dos Genitais Masculinos/microbiologia , Papillomavirus Humano/genética , Infecções Tumorais por Vírus/microbiologia , Papillomavirus Humano/fisiologiaRESUMO
Human Papillomaviruses (HPV) are the cause of benign human anogenital lesions where HPV 6 and HPV 11 are most commonly found. Conversely, HPV 16, 18, 31 and 33 are frequently detected in genital carcinomas and are thus considered as oncogenic types. In order to analyze the prevalence of specific HPV types in an Argentine male population, 43 anogenital lesions from different patients with diagnosis of condyloma acuminata were analyzed. These lesions were localized in different regions of the male genitalia comprising the corona glandis, urethral meatus, skin of the penis, scrotum and anus. The biopsies were screened for the presence of HPV 6, 11, 16, 18, 30, 31 and 33 by Southern blot at different stringent conditions of hybridization (Tm -48 degrees C and Tm -20 degrees C). HPV DNA was found in 41 examined cases (95.3%) with a clear prevalence of HPV 6 and HPV 11 types (51.2% and 23.3% respectively). Six samples (14.0%) were positive only under nonstringent conditions of hybridization. Mixed infections between HPV 16, 18, 30, 31, 33 or a HPV 30 related type with HPV 6 or HPV 11 were detected in 8 specimens (18.6%). Only one case was between HPV 16 and HPV 30. Two additional samples were only positive for HPV 30. Experiments in progress about the prevalence of HPV types in female lesions as well as in normal subjects will contribute to complete the description of the epidemiology of these infections in Argentina.
Assuntos
Condiloma Acuminado/microbiologia , DNA Viral/análise , Doenças dos Genitais Masculinos/microbiologia , Papillomaviridae/genética , Infecções Tumorais por Vírus/microbiologia , Adulto , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Papillomaviridae/fisiologiaRESUMO
Human Papillomaviruses (HPV) are the cause of benign human anogenital lesions where HPV 6 and HPV 11 are most commonly found. Conversely, HPV 16, 18, 31 and 33 are frequently detected in genital carcinomas and are thus considered as oncogenic types. In order to analyze the prevalence of specific HPV types in an Argentine male population, 43 anogenital lesions from different patients with diagnosis of condyloma acuminata were analyzed. These lesions were localized in different regions of the male genitalia comprising the corona glandis, urethral meatus, skin of the penis, scrotum and anus. The biopsies were screened for the presence of HPV 6, 11, 16, 18, 30, 31 and 33 by Southern blot at different stringent conditions of hybridization (Tm -48 degrees C and Tm -20 degrees C). HPV DNA was found in 41 examined cases (95.3
) with a clear prevalence of HPV 6 and HPV 11 types (51.2
and 23.3
respectively). Six samples (14.0
) were positive only under nonstringent conditions of hybridization. Mixed infections between HPV 16, 18, 30, 31, 33 or a HPV 30 related type with HPV 6 or HPV 11 were detected in 8 specimens (18.6
). Only one case was between HPV 16 and HPV 30. Two additional samples were only positive for HPV 30. Experiments in progress about the prevalence of HPV types in female lesions as well as in normal subjects will contribute to complete the description of the epidemiology of these infections in Argentina.
Assuntos
Candidíase/diagnóstico , Doenças em Gêmeos/diagnóstico , Epidermólise Bolhosa Juncional/diagnóstico , Doenças do Prematuro/diagnóstico , Pneumonia/diagnóstico , Infecções Estafilocócicas/diagnóstico , Estômago/anormalidades , Adulto , Epidermólise Bolhosa Juncional/complicações , Feminino , Humanos , Recém-Nascido , Pneumonia/complicaçõesRESUMO
Human papillomavirus type 6 subtype a (HPV-6a) was detected in a human invasive tonsillar carcinoma. Southern blot hybridization analysis showed the presence of additional bands when using non-cutting and single-cut restriction enzymes. Molecular cloning yielded two recombinant clones of 8.0 and 1.4 kb in size. The first represents the complete HPV-6a genome. Sequence analysis of the second clone showed a 0.6 kb DNA sequence corresponding to the L2 region of HPV-6a, whereas the rest belongs to cellular sequences. These data show the presence of a usually low risk HPV type in an invasive carcinoma, at an unusual infection site, with viral DNA integrated into the host genome. These findings add evidence in support of the hypothesis of a relationship between HPV infection and at least some ororespiratory cancers.