RESUMO

Oligophenylalanines are soluble in m-cresol, but oligophenylalanyl-tRNAs are not. This differential solubility can be used to assay oligophenylalanines released from tRNA during their synthesis by wheat germ extracts. When poly U is the message, virtually no free product appears. When poly A U (A < U) is used, a considerable amount of oligophenylalanines are released. The fraction of product released is approximately constant with time, implying that a steady-state is not achieved between initiation and release. The dependence of release on various reaction variables and the effects of several inhibitors on release indicate that the reaction is probably catalyzed by peptidyl transferase, in accord with the mechanisms described for mammals and prokaryotes.

Assuntos

Oligopeptídeos/metabolismo , Terminação Traducional da Cadeia Peptídica , Fenilalanina/análogos & derivados , Sistema Livre de Células , Códon/metabolismo , Cicloeximida/farmacologia , Ácido Fusídico/farmacologia , Guanosina Trifosfato/análogos & derivados , Guanosina Trifosfato/farmacologia , Lisina/metabolismo , Metanol/farmacologia , Oligopeptídeos/biossíntese , Oligopeptídeos/isolamento & purificação , Terminação Traducional da Cadeia Peptídica/efeitos dos fármacos , Fenilalanina/biossíntese , Fenilalanina/isolamento & purificação , Fenilalanina/metabolismo , Poli A-U/metabolismo , Poli U/metabolismo , Biossíntese de Proteínas/efeitos dos fármacos , Puromicina/farmacologia , Solubilidade , Esparsomicina/farmacologia , Triticum