RESUMO
Photochemical reactions are major pathways for the removal of Hg species from aquatic ecosystems, lowering the concentration of monomethylmercury (MMHg) and its bioaccumulation in foodwebs. Here, we investigated the rates and environmental drivers of MMHg photodegradation and inorganic Hg (IHg) photoreduction in waters of two high-altitude lakes from the Bolivian Altiplano representing meso- to eutrophic conditions. We incubated three contrasting waters in situ at two depths after adding Hg-enriched isotopic species to derive rate constants. We found that transformations mostly occurred in subsurface waters exposed to UV radiation and were mainly modulated by the dissolved organic matter (DOM) level. In parallel, we incubated the same waters after the addition of low concentrations of natural MMHg and followed the stable isotope composition of the remaining Hg species by compound-specific isotope analysis allowing the determination of enrichment factors and mass-independent fractionation (MIF) slopes (Δ199Hg/Δ201Hg) during in situ MMHg photodegradation in natural waters. We found that MIF enrichment factors potentially range from -11 to -19 and average -14.3 ± 0.6 (1 SE). The MIF slope diverged depending on the DOM level, ranging from 1.24 ± 0.03 to 1.34 ± 0.02 for the low and high DOM waters, respectively, and matched the MMHg MIF slope recorded in fish from the same lake. Our in situ results thus reveal (i) a relatively similar extent of Hg isotopic fractionation during MMHg photodegradation among contrasted natural waters and compared to previous laboratory experiments and (ii) that the MMHg MIF recorded in fish is characteristic for the MMHg bonding environment. They will enable a better assessment of the extent and conditions conducive to MMHg photodegradation in aquatic ecosystems.
Assuntos
Mercúrio , Compostos de Metilmercúrio , Poluentes Químicos da Água , Altitude , Animais , Bolívia , Ecossistema , Monitoramento Ambiental , Peixes/metabolismo , Isótopos , Lagos/química , Mercúrio/análise , Isótopos de Mercúrio/análise , Compostos de Metilmercúrio/metabolismo , Água/metabolismo , Poluentes Químicos da Água/análiseRESUMO
Periphyton relevance for methylmercury (MeHg) production and accumulation are now well known in aquatic ecosystems. Sulfate-reducing bacteria and other microbial groups were identified as the main MeHg producers, but the effect of periphyton algae on the accumulation and transfer of MeHg to the food web remains little studied. Here we investigated the role of specific groups of algae on MeHg accumulation in the periphyton of Schoenoplectus californicus ssp. (Totora) and Myriophyllum sp. in Uru Uru, a tropical high-altitude Bolivian lake with substantial fishing and mining activities accruing around it. MeHg concentrations were most strongly related to the cell abundance of the Chlorophyte genus Oedogonium (r 2 = 0.783, p = 0.0126) and to no other specific genus despite the presence of other 34 genera identified. MeHg was also related to total chlorophyll-a (total algae) (r 2 = 0.675, p = 0.0459), but relations were more significant with chlorophyte cell numbers, chlorophyll-b (chlorophytes), and chlorophyll-c (diatoms and dinoflagellates) (r 2 = 0.72, p = 0.028, r 2 = 0.744, p = 0.0214, and r 2 = 0.766, p = 0.0161 respectively). However, Oedogonium explains most variability of chlorophytes and chlorophyll-c (r 2 = 0.856, p = < 0.001 and r 2 = 0.619, p = 0.002, respectively), suggesting it is the most influential group for MeHg accumulation and periphyton algae composition at this particular location and given time.
Assuntos
Magnoliopsida/fisiologia , Compostos de Metilmercúrio/metabolismo , Microalgas/química , Poluentes Químicos da Água/metabolismo , Altitude , Biota , Bolívia , Clorófitas/química , Clorófitas/classificação , Cyperaceae/fisiologia , Diatomáceas/química , Diatomáceas/classificação , Dinoflagellida/química , Dinoflagellida/classificação , Monitoramento Ambiental , Lagos , Microalgas/classificaçãoRESUMO
Tropical high-altitude Andean lakes are physically harsh ecosystems. Located above the treeline (≥4000 m a.s.l.), they share common features with temperate alpine lakes, which impose extreme conditions on their aquatic organisms: e.g., strong winds, broad diel variations in water temperature, and intense solar ultraviolet radiation (UVR). However, because of their latitude, they differ in two major ecological characteristics: they lack ice cover during the winter and they do not present summer water column stratification. We sampled 26 tropical high-altitude Andean lakes from three regions of the Bolivian Eastern Andes Cordillera during the wet period (austral summer). We performed an ordination to better describe the typology of Andean lakes in relation to the environmental variables, and we assessed the relationships among them, focussing on the UV-A transparency (360 nm) throughout the water column. We found a positive correlation between UV-A transparency calculated as Z(1%) (the depth which reaches 1% of the surface UV-A), the lake maximum depth and Secchi transparency (r = 0.61). Z(1%) of UV-A was smaller in shallow lakes than in deep lakes, indicating that shallow lakes are less transparent to UV-A than deep lakes. We hypothesize that, compared to shallow lakes, deep lakes (maximum depth > 10 m) may have lower dissolved organic carbon (DOC) concentrations (that absorb UV radiation) due to lower temperature and reduced macrophyte cover. Based on our data, tropical high-altitude Andean lakes are less transparent to UV-A (K(d) range = 1.4-11.0 m(-1); Z(1%) depth range = 0.4-3.2 m) than typical temperate alpine lakes (1-6 m(-1), 3-45 m, respectively). Moreover, they differ in vertical profiles of UV-A, chlorophyll-a, and temperature, suggesting that they may have a distinct ecological functioning. Such peculiarities justify treating tropical high-altitude Andean lakes as a separate category of alpine lakes. Tropical high-altitude Andean lakes have been poorly studied. Thus they deserve more in-depth studies in the face of global changes regarding the use of their UV transparency as a sentinel proxy of climate changes, particularly global warming.
Assuntos
Lagos , Raios Ultravioleta , Altitude , Bolívia , Mudança Climática , Ecossistema , Lagos/química , TemperaturaRESUMO
Un certain nombre de travaux ont ete reallisses sur l'hydrologie, la physico-chimie, l'algologie, les productons primaire et secondaire ainsi que sur l'ichtylogie du Grand lac (bassin nord profond du Lac Titicaca ou Lago Mayor, of. Figure 1). On peut citer, en particulier, ceux de Gilson (1939,1940,1955), MONHEIM (1956), WIDMER et al.(1975), RICHERSON et al. (1977), CARMOUZE et al. (l977, 1978,1980,1981), REYSSAC et DAO (1977).Le but de la presente etude est de donner les caracteristiques originales du phytoplancton d'un lac tropical de tres haute altitude ,en l'occurence le Lac Titicaca, situe par 16ª de latitude sud a 3808 metres d'altitude dans les Andes, sur le plan des peuplements, des biomasses et de la producction primaire. Le Lac Titicaca se divise en deux bassins qui communiquent par un detroit: le Grand Lac et le Petit Lac.Seul, ce dernier fait l' objet de la presente etude. Une comparaison entre les resultats obtenus sur le Petit Lac (appele Lago Menor ou encore Lago Huiñaimarca) et des travaux enterieurs concernant le Grand Lac, ainsi que d' autres milieux connus (tropicaux, termperes, d'autres milieux connus (tropicaux, temperes,d'altitude) est tantee, de meme une analyse de son etat trophique...