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BACKGROUND: The indigenous population located in the central region of Brazil, is the second largest in terms of population size in the country. The Indigenous Reserve of Dourados has risk factors that increase the vulnerability of the indigenous population to infectious diseases, especially Human alphaherpesvirus (HSV-1), a neglected disease with high prevalence in priority populations in developing countries. The virus can also cause many more severe diseases, including widespread neonatal infections, herpetic keratitis, and herpes encephalitis, which can be fatal if left untreated. We estimated the prevalence of anti-HSV-1 antibodies and correlated it with the demographic and behavioral characteristics of the Indigenous population of the Jaguapirú and Bororó villages (Dourados, Mato Grosso do Sul (MS), Brazil). METHODS: Our approach was cross-sectional. From March 2017 to November 2018. Using anti-HSV-1 (Gg1) IgM and anti-HSV-1 (gG1) IgG Euroimmun and the detection and quantification of HSV-1 viral load in plasma samples, through real-time PCR. The maps were constructed using QGIS and the statistical analyses using R Studio software. RESULTS: A total of 1138 individuals (> 18 years old) were enrolled. The prevalence of anti-HSV-1 IgM and IgG were 20% and 97.5%, respectively. The prevalence of anti-HSV-1 antibodies for IgG was higher in both sexes. Anti-HSV-1 IgM antibodies were present in 17.1%, 21.2%, 12.5%, and 22% of the participants with urinary problems, genital wounds, genital warts, and urethral discharge, respectively. Real-time PCR was used for confirmatory testing; HSV-1 DNA was detected in 25.6% (54/211) of anti-HSV1 IgM-positive samples. Viral loads ranged from 5.99E + 02 to 3.36E + 13. CONCLUSIONS: The seroprevalence of HSV-1 IgM and detection of HSV-1 DNA in the Indigenous population confirmed high silent prevalence. Furthermore, the seroprevalence of HSV-1 in the Indigenous population was higher than that reported in the general adult Brazilian population. Various socioeconomic factors, drug use, and health and sexual behaviors could contribute to the facilitation of HSV-1 transmission in the Indigenous population. Our results may help develop culturally appropriate intervention programs that eliminate health access barriers and improve the implementation of public health policies aimed at promoting information regarding the prevention, treatment, and control of HSV-1 infection in Brazilian Indigenous populations.
Assuntos
Anticorpos Antivirais , Herpes Simples , Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Anticorpos Antivirais/sangue , Brasil/epidemiologia , Estudos Transversais , Herpes Simples/epidemiologia , Herpes Simples/virologia , Herpesvirus Humano 1/imunologia , Herpesvirus Humano 1/genética , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Indígenas Sul-Americanos/estatística & dados numéricos , Prevalência , Carga ViralRESUMO
A pesquisa qualitativa em pedagogia do esporte conquistou reconhecimento acadêmico, mas ainda pairam dúvidas sobre sua aleatoriedade, subjetividade e falta de rigor com base na ética do/a pesquisador/a. Defendemos, apoiado em reflexões filosóficas, posição favorável à busca por rigorosidade metodológica na pesquisa qualitativa, porém, nossa posição não se baseia na cisão, mas no diálogo possível entre as premissas positivistas que sustentam a busca por rigorosidade e a possibilidade de pleno envolvimento do/a pesquisador/a com o campo de pesquisa. Diante deste contexto, apresentamos os nossos esforços na condução de um estudo de pós-graduação sobre competições de jovens, apresentando os caminhos metodológicos percorridos em busca de validação e confiabilidade dos procedimentos de coleta de informações e tratamento analítico aos dados qualitativos.
Qualitative research in sport pedagogy has conquered academic recognition, but doubts about its randomness, subjectivity and lack of rigor based on researcher ethics. We defend, based on philosophical reflections, a position favorable to the adoption of criteria of methodological rigor for qualitative research, however, our position is not based on a split, but on the possible dialogue between the positivist premises that support the search for rigor and the possibility of full involviment of the researcher with de research field. Thus, we present our efforts in conducting a postgraduate study on youth competitions, presenting the methodological paths traveled in search of validation and reliability for data collection procedures and analytical treatment of qualitative data.
La investigación cualitativa en pedagogía del deporte ha conquistado reconocimiento académico, pero aún quedan dudas sobre su aleatoriedad, subjetividad y falta de rigor a partir de la ética del investigador. Defendemos, con base en reflexiones filosóficas, una posición favorable a la adopción de criterios de rigor metodológico para la investigación cualitativa, sin embargo, nuestra posición no se basa en una escisión, sino en el diálogo posible entre las premisas positivistas que sustentan la búsqueda de rigor y la posibilidad de total involucramiento del investigador com el campo de investigación. Así, presentamos nuestros esfuerzos en la realización de un estudio de posgrado sobre competiciones juveniles, presentando los caminos metodológicos recorridos en busca de la validación y confiabilidad para los procedimientos de recolección de informaciónes y tratamiento analítico a los datos cualitativos.
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Com o presente artigo pretendemos problematizar e explorar o conceito de jogo, entendendo-o como algo amplo, rizomático e não fechado em uma única forma de interpretação. Além do que, o conceito de jogo pode já ter sido cristalizado em decorrência da sua consolidação, assim, seu desenvolvimento só se torna possível na medida em que se há um arruinamento para devir novas potencialidades. Foi, então, a partir dos pensamentos de Deleuze e Guattari sobre: conceito e rizoma, de onde partimos para desenvolver o conceito de macro-jogo e microjogo, em uma tentativa de potencializar as discussões por um viés interdisciplinar e de cunho filosófico. Diante de tais questões, foi possível compreender a importância do olhar interdisciplinar para a resolução de problemas provenientes de áreas distintas do conhecimento, rompendo com a dicotomia entre teoria e prática tão evidente e presente em diversas áreas do conhecimento, entre elas, a Educação Física e as artes
Our intend with this article is to question and explore the concept of game, understanding it as something broad, rhizomatic and not closed in a single form of interpretation. In addition, the game concept may have already been crystallized as a result of its consolidation, thus, its development only becomes possible as long as new potentialities are ruined. It was, then, from the thoughts of Deleuze and Guattari on concept and rhizome, from which we started to develop the concept of macro-game and micro-game, in an attempt to enhance the discussions through an interdisciplinary and philosophical approach. Facing these questions, it was possible to understand the importance of the interdisciplinary look to solve problems coming from different areas of knowledge, breaking the dichotomy between theory and practice so evident and present in several areas of knowledge, including Physical Education and the arts.
Con el presente artículo pretendemos problematizar y explorar el concepto de juego, entendiéndolo como algo amplio, rizomático y no cerrado en una única forma de interpretación. Además, el concepto de juego puede haber sido ya cristalizado como resultado de su consolidación, por lo que su desarrollo sólo se hace posible en la medida en que hay una ruína para idear nuevas potencialidades. Se basó, pues, en las reflexiones de Deleuze y Guattari sobre el concepto y el rizoma, a partir de las cuales desarrollamos el concepto de macro-juego y micro-juego, en un intento de enriquecer los debates mediante un enfoque interdisciplinario y filosófico. Ante estas cuestiones, se pudo comprender la importancia de la mirada interdisciplinaria para resolver los problemas que surgen de las diferentes áreas del conocimiento, rompiendo la dicotomía entre teoría y práctica tan evidente y presente en várias áreas del conocimiento, incluyendo la Educación Física y las artes.
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The malignant neoplastic cell is characterized by its diverse metabolic changes. It occurs in order to maintain the high rate of proliferation. The possibility of new pharmacological targets has inserted tumor metabolism as a target for recent research, emphasizing the enzymatic activity of thiamin. This review aims to elucidate the behavior of thiamin against tumor development. This is a systematic review in which studies indexed in Pubmed, Scopus, SciELO and BVS were searched using the descriptors (Thiamin OR Vitamin B1) AND (Cancer OR Malignant neoplasia) AND (Tumor metabolism). Title and abstract were read. Duplicates, literary reviews, books, conference abstracts, editorials, and papers published prior to 2010 were eliminated. 23 records were included in this review. Low doses of thiamin have been shown to be enough to stimulate tumor growth. Another population studies has shown evidence of tumor regression after correction of vitamin B1 deficiency. There is an open path for the development of new research to better assess the influence of thiamin on cancer cells. Once the connections between thiamin and the metabolism of cancer cells are fully established, new opportunities for therapeutic intervention and dietary modification will appear to reduce the progression of the disease in patients with cancer.
Assuntos
Neoplasias , Deficiência de Tiamina , Carcinogênese , Transformação Celular Neoplásica , Suplementos Nutricionais , Humanos , Neoplasias/tratamento farmacológico , Tiamina/farmacologia , Deficiência de Tiamina/tratamento farmacológico , Deficiência de Tiamina/metabolismoRESUMO
Objetivo: avaliar a eficácia da utilização da terapia combinada de alendronato de sódio e vitamina D no metabolismo ósseo de mulheres em tratamento de osteoporose pós-menopausa. Métodos: trata-se de uma revisão sistemática, a qual foram pesquisados ensaios clínicos randomizados (ECR) indexados nas bases de dados BVS, ISI Web of Science, PubMed, SciELO, ScienceDirect e Scopus que comparavam a associação de alendronato sódico e vitamina D com a monoterapia de alendronato de sódio. Resultados: um total de seis ECR contemplou os critérios para serem inclusos nesse estudo, compreendendo um total de 4164 participantes e seus respectivos dados. Os estudos avaliaram diferentes domínios do metabolismo ósseo, como níveis séricos de vitamina D, paratormônio, densidade mineral óssea e marcadores de turnover ósseo. A terapia combinada produziu melhora significativa nos marcadores metabólicos ósseos. Conclusão: a terapia combinada de alendronato de sódio com vitamina D promove melhora no metabolismo ósseo de mulheres com osteoporose pós-menopausa.
Aim: to evaluate the effectiveness of using the combined therapy of sodium alendronate and vitamin D on bone metabolism in women undergoing postmenopausal osteoporosis. Methods: this is a systematic review. The studies included were Randomized Controlled Trials (RCT) indexed in the BVS, ISI Web of Science, PubMed, SciELO, ScienceDirect and Scopus Databases which compared the association of sodium alendronate and vitamin D to monotherapy of sodium alendronate. Results: a total of six RCT met the criteria to be included in this study, comprising a total of 4164 participants and their respective data. The studies evaluated different domains of bone metabolism, such as serum levels of vitamin D, parathyroid hormone, bone mineral density and bone turnover markers. Combination therapy produced significant improvement in bone metabolic markers. Conclusion: combined therapy of sodium alendronate with vitamin D promotes improved bone metabolism in women with postmenopausal osteoporosis.
Assuntos
Humanos , Feminino , Hormônio Paratireóideo , Vitamina D , Mulheres , Osso e Ossos , Densidade Óssea , Osteoporose Pós-Menopausa , AlendronatoRESUMO
Partimos da crítica à tendência utilitarista das abordagens baseadas no jogo (GBAs) com o objetivo de analisar como as dimensões éticas e morais se revelam no "ato de jogar" a partir da prática pedagógica do(a) treinador(a) dos esportes coletivos. Sustentados nos conceitos do "jogar" ético e do "esportear" moral, defendemos 1) a primazia do jogar sobre o esportear, 2) a necessidade de que os sentimentos éticos, manifestos pelo estado de jogo, passem pelo crivo da norma, representada pela atitude lusória e 3) que atitudes transgressoras e subversivas à regra sejam analisadas em função de sua fonte: amor a si ou resistência aos impasses promovidos pelas regras. Concluímos que o ato de jogar revela por si mesmo o plano ético-moral inerente ao jogo/esporte, sendo a instrumentalização do jogo com um comprometimento exclusivamente voltado à dimensão moral normativa um risco à dimensão ética inerente ao papel do(a) jogador(a). (AU)
We start from the critique of the utilitarian tendency of game-based approaches (GBAs) with the objective of analyzing how the ethical and moral dimensions are revealed in the "act of playing" from the coaches' pedagogical practice. Through the concepts of ethical "to play" and moral "to sport", we support 1) the primacy of playing over sporting, 2) the need for ethical feelings, manifested by the "state of play", to pass through the scrutiny of the "lusory attitude" and 3) that transgressive and subversive attitudes to the rule are considerable depending on their source: self-love or resistance to the impasses promoted by the rules. We conclude that the "act of playing" reveals by itself the ethical-moral plan inherent to the game/sport, and the instrumentalization of the game with a commitment exclusively focused on the normative moral dimension is a risk to the ethical dimension inherent to the role of the player.(AU)
Partimos de la crítica a la tendencia utilitarista de los enfoques basados en el juego (GBAs) con el objetivo de analizar cómo se revelan las dimensiones éticas y morales en el "acto de jugar" a partir de la práctica pedagógica del/la entrenador/a de deportes colectivos. A partir de los conceptos del "jugar" ético y del "deportear" moral, defendemos 1) la primacía del jugar sobre el "deportear," 2) la necesidad de que los sentimientos éticos, puestos de manifiesto por el estado de juego, pasen por el tamiz de la norma, representada por la actitud lusoria y 3) que las actitudes transgresoras y subversivas a la regla sean analizadas según su origen: amor propio o resistencia a los impasses promovidos por las reglas. Concluimos que el acto de jugar revela por sí mismo el plano ético-moral inherente al juego/deporte, y la instrumentalización del juego con un compromiso centrado exclusivamente en la dimensión moral normativa es un riesgo para la dimensión ética inherente al rol del jugador.(AU)
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Humanos , Masculino , Feminino , Esportes de EquipeRESUMO
BACKGROUND: People with good health knowledge present a conceptual and objective appropriation of general and specific health topics, increasing their probability to express health protection and prevention measures. The main objective of this study was to conduct a rapid systematic review about the effects of health knowledge on the adoption of health behaviors and attitudes in populations under pandemic emergencies. METHODS: A systematic review was performed according to PRISMA checklist and the Cochrane method for rapid systematic reviews. Studies searches were performed in APA PsycNet, Embase, Cochrane Library and PubMed Central. Studies published between January 2009 and June 2020 and whose primary results reported a measure of interaction between health knowledge, health attitudes and behaviors in population groups during pandemics were included. A review protocol was recorded in PROSPERO (CRD42020183347). RESULTS: Out of a total of 5791 studies identified in the databases, 13 met the inclusion criteria. The included studies contain a population of 26099 adults, grouped into cohorts of health workers, university students, clinical patients, and the general population. Health knowledge has an important influence on the adoption of health behaviors and attitudes in pandemic contexts. CONCLUSIONS: The consolidation of these preventive measures favors the consolidation of public rapid responses to infection outbreaks. Findings of this review indicate that health knowledge notably favors adoption of health behaviors and practices. Therefore, health knowledge based on clear and objective information would help them understand and adopt rapid responses to face a pandemic.
Assuntos
COVID-19/epidemiologia , Emergências/epidemiologia , Comportamentos Relacionados com a Saúde/fisiologia , Conhecimentos, Atitudes e Prática em Saúde , COVID-19/virologia , Humanos , Pandemias/prevenção & controle , Fatores de RiscoRESUMO
O esporte e a competição são inseparáveis, estão presentes na vida dos jovens que praticam esportes e, por isso, é necessário criar ambientes favoráveis ao desenvolvimento desses indi-víduos. O objetivo desta pesquisa foi conhecer as percepções dos treinadores de handebol de elite quanto aos aspectos da promoção de competições para jovens. Quatro treinadores de elite foram entrevistados e, a partir da análise qualitativa das entrevistas, foi possível dividir os resultados em quatro categorias: a) Adaptações para competições; b) Utilização do sistema defensivo individual; c) Papel do treinador; e d) Vitória como foco nas competições de base. Foram apresentadas necessidades e sugestões para criar um ambiente competitivo favorável e pressupostos à atuação do treinador de base.
Sport and competition are inseparable and are present in the lives of young people who play sports and therefore it is necessary to create environments conducive to the development of them. The objective of this research was to know the perceptions of elite handball coaches regarding the premises associated with the promotion of youth competitions. Four elite coaches were interviewed and, from the qualitative analyses, it was possible to divide the re-sults into four categories: a) Adaptations for competitions; b) Use of the individual defensive system; c) Role of the coach; and d) Victory as a focus on young competitions. Requirements and suggestions were presented to create a favourable competitive environment, in addition to assumptions the performance of the young coaches.
El deporte y la competición son inseparables y están presentes en la vida de los jóvenes que practican deportes y, por eso, es necesario crear ambientes favorables al desarrollo de esos individuos. El objetivo de esta investigación fue conocer las percepciones de los entrenadores de balonmano de elite como las premisas asociadas a competiciones para jóvenes. Cuatro en-trenadores fueron entrevistados y fue posible dividir los resultados en cuatro categorías: a) Adaptaciones para competiciones; b) utilización del sistema defensivo individual; c) Papel del entrenador; y d) Victoria como foco en las competiciones de base. Se presentaron necesidades y sugerenciaspara crear un ambiente competitivo favorable, además de presupuestos para la actuación del entrenador de base.
Assuntos
Humanos , Esportes , Ensino , AtletasRESUMO
Pfaffia glomerata is a medically important species because it produces the phytoecdysteroid 20-hydroxyecdysone (20-E). However, there has been no ready-to-use transcriptome data available in the literature for this plant. Here, we present de novo transcriptome sequencing of RNA from P. glomerata in order to investigate the 20-E production as well as to understand the biochemical pathway of secondary metabolites in this non-model species. We then analyze the effect of photoautotrophy on the production of 20-E genes phylogenetically identified followed by expression analysis. For this, total messenger RNA (mRNA) from leaves, stems, roots, and flowers was used to construct indexed mRNA libraries. Based on the similarity searches against plant non-redundant protein database, gene ontology, and eukaryotic orthologous groups, 164,439 transcripts were annotated. In addition, the effect of photoautotrophy in two genes putatively involved in the 20-E synthesis pathway was analyzed. The Phantom gene (CYP76C), a precursor of the route, showed increased expression in P. glomerata plants cultured under photoautotrophic conditions. This was accompanied by increased production of this metabolite indicating a putative involvement in 20-E synthesis. This work reveals that several genes in the P. glomerata transcriptome are related to secondary metabolism and stresses, that genes of the P450 family participate in the 20-E biosynthesis route, and that plants cultured under photoautotrophic conditions promote an upregulated Phantom gene and enhance the productivity of 20-E. The data will be used for future investigations of the 20-E synthesis pathway in P. glomerata while offering a better understanding of the metabolism of the species.
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Amaranthaceae/genética , Processos Autotróficos , Sistema Enzimático do Citocromo P-450/genética , Ecdisterona/biossíntese , Genes de Plantas , Família Multigênica , Processos Fototróficos , Transcriptoma/genética , Processos Autotróficos/genética , Vias Biossintéticas/genética , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Anotação de Sequência Molecular , Fases de Leitura Aberta/genética , Processos Fototróficos/genética , Filogenia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reprodutibilidade dos Testes , Análise de Sequência de RNARESUMO
Aim: To investigate the speeches of elite handball coaches about the current strategic-tactical configuration after the rule change that allows the use of the seventh court player in the attack. Methods: Four elite handball coaches were interviewed (semi-structured) with specific questions about the main theme of this study. Coaches' speeches were analyzed using the Qualitative Content Analysis. Results: Three categories of analysis emerged from coaches' discourses: 1) a general overview of the current tactical application of the seventh court player: coaches report that there is no significant change in strategic-tactical structures previously established in handball; 2) main tactical strategies from the use of the rule change: the seventh court player used, mainly, like additional player in exclusion situations to maintain the numerical equality in the offensive phase, and to numerical superiority of the attack in the final minutes by the teams that are losing; and 3) main perspectives of change in the offensive, defensive and transitions systems, in which there is a concern with the restriction of the use of defensive system with open formation. Conclusion: there were no significant strategic changes in handball in attack or defense, and the use of the additional court player was used to maintain the numerical equality in the attack in situations of exclusion and, in some specific cases, aiming at numerical superiority in final and decisive moments of matches.(AU)