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Biomed Chromatogr ; 32(2)2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28913959

RESUMO

The rise of the mosquitoes-transmitted diseases, like dengue, zika and chikungunya in Brazil in the last years has increased concerns on protection against mosquitoes bites. However, the prohibitive prices of the commercially available repellents for the majority of the Brazilian population has provoked a search for cheaper solutions, like the use of the homemade ethanolic extract of Indian clove (Syzygium aromaticum L.) as repellent, which has been reported as quite efficient by the local press. In order to verify this, we performed here the quantification of the main components of this extract through high-performance thin-layer chromatography (HPTLC)-densitometry and evaluated its efficiency as a repellent and its acetylcholinesterase (AChE) inhibition capacity. Our results have proved HPTLC-densitometry as an efficient and appropriate method for this quantification and confirmed the repellency activity, as well as its capacity of AChE inhibition.


Assuntos
Inibidores da Colinesterase , Cromatografia em Camada Fina/métodos , Repelentes de Insetos , Extratos Vegetais , Syzygium/química , Acetilcolinesterase/metabolismo , Adulto , Aedes/efeitos dos fármacos , Animais , Comportamento Animal/efeitos dos fármacos , Inibidores da Colinesterase/análise , Inibidores da Colinesterase/química , Inibidores da Colinesterase/farmacologia , Densitometria , Eugenol/análise , Eugenol/química , Eugenol/farmacologia , Humanos , Repelentes de Insetos/análise , Repelentes de Insetos/química , Repelentes de Insetos/farmacologia , Limite de Detecção , Modelos Lineares , Masculino , Extratos Vegetais/análise , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Reprodutibilidade dos Testes , Adulto Jovem
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