RESUMO
This study aims to investigate the correlation between allergic sensitization of atopic dermatitis (AD) patients and their serum interleukin (IL)-16 levels. AD patients, healthy volunteers, and patients with psoriasis (N = 80, 35, 20, respectively) were tested for serum IL-16 and total and specific IgE levels by enzyme-linked immunosorbent assay, along with eosinophil counts. Serum allergen-specific IgE levels were determined, and skin-prick testing conducted in a subgroup of 45 AD patients. Based on specific IgE levels, AD patients were categorized into non-sensitized group 1 and sensitized group 2. Furthermore, they were sorted as non-sensitized group A and sensitized group B based on skin-prick results. Next, the serum IL-16 and total IgE levels in these subgroups were determined. Compared to levels in healthy volunteers and psoriasis patients, the serum IL-16 levels in AD patients were significantly higher (P < 0.001). Additionally, total serum IgE levels were significantly correlated with serum IL-16 levels and eosinophil counts. However, no correlation was observed between serum IL-16 levels and eosinophil counts. The serum IL-16 and total IgE levels in group 2 were also significantly elevated (P < 0.001) in contrast to those in group 1. Although we did not observe any significant difference between serum IL-16 levels in groups A and B, the total serum IgE level in group B was significantly higher than that in group A (P < 0.001). Thus, allergic sensitivity in AD patients correlates with total serum IgE as well as serum IL-16; the correlation with IL-6 is weaker.
Assuntos
Dermatite Atópica/sangue , Interleucina-16/sangue , Adolescente , Adulto , Criança , Ensaio de Imunoadsorção Enzimática , Eosinófilos/citologia , Feminino , Humanos , Imunoglobulina E/sangue , Masculino , Pessoa de Meia-Idade , Psoríase/sangue , Adulto JovemRESUMO
The clinical significance of serum S-100ß levels in neonates with hypoxic-ischemic encephalopathy (HIE), as a reference index to assess HIE severity, was evaluated in this study. On the basis of our strict inclusion and exclusion criteria, relevant high-quality case-control studies reporting the association between HIE and S-100ß protein were selected from electronic database searches. The STATA version 12.0 software was used for the statistical analyses. The database search initially retrieved 93 studies (37 in English and 56 in Chinese), and following a multistep screening process, 13 high-quality studies were eventually included in our meta-analysis. The 13 case-control studies included a total of 646 HIE neonates and 381 healthy controls. The results of this meta-analysis revealed that serum S-100ß levels in mild, moderate, and severe HIE neonates were significantly higher than those in healthy controls, and the differences were statistically significant. Importantly, the serum S-100ß levels increased incrementally with HIE severity. Our results support the hypothesis that S-100ß is an important biological indicator of HIE and serum S-100ß levels can be used as a reference index to assess HIE severity.
Assuntos
Estudos de Associação Genética , Hipóxia-Isquemia Encefálica/genética , Subunidade beta da Proteína Ligante de Cálcio S100/sangue , Estudos de Casos e Controles , Feminino , Humanos , Hipóxia-Isquemia Encefálica/sangue , Hipóxia-Isquemia Encefálica/patologia , Recém-Nascido , Subunidade beta da Proteína Ligante de Cálcio S100/genética , Índice de Gravidade de DoençaRESUMO
The coconut leaf beetle, Brontispa longissima, is a destructive pest of palm plants. Although its ecological and biological characteristics are well understood, its genetic information remains largely unknown. To advance our understanding of its molecular ba-sis of biology and ecology, we sequenced and analyzed its whole transcriptome by using high-throughput Illumina paired-end sequencing technology. Approximately 8.08 Gb of clean reads were generated in a single run, which were assembled by using Trinity into 41,652 unigenes with an average length of 932 bp. By sequence similarity searches for known proteins, 23,077 (55.4%) unigenes were annotated by BLASTx searches against the NCBI non-redundant protein database. Of the unigenes assembled, 18,153 and 13,733 were assigned to Gene Ontology and Clusters of Orthologous Groups of proteins, respectively. In addition, 10,415 unigenes were mapped onto 247 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. These transcriptomic resources will facilitate gene identification and elucidate the molecular mechanisms of biological and ecological aspects under-lying this palm pest, in order to design a new control strategy.
Assuntos
Besouros/genética , Sequenciamento de Nucleotídeos em Larga Escala , Transcriptoma/genética , Animais , Besouros/patogenicidade , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Repetições de Microssatélites/genética , Anotação de Sequência Molecular , Controle Biológico de Vetores , Phoeniceae/parasitologia , Folhas de Planta/parasitologiaRESUMO
Olfaction is crucial for insects' survival because it enables them to recognize various environmental information. It is primarily mediated by a large family of chemoreceptors, including olfactory receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs). Here, we assembled the transcriptome of the economically important pest of palms, Rhynchophorus ferrugineus, to reveal its chemoreceptor gene repertoire. About 8.08 Gbp data were generated using a HiSeq platform and their assembly led to a total of 24,439 unigenes. Among the transcripts, 12,523 (51.24%) showed significant similarity (E-value <10(-5)) to known proteins in the National Center for Biotechnology Information Nr database. From these sequences, 18 candidate genes of ORs were identified. Nine putative transcripts were homologous to GR genes, while 9 were similar to IR genes. The expression profiles of all identified chemoreceptor genes were determined by quantitative real-time PCR in antenna, head, thorax, abdomen, and legs of both sexes. Most chemoreceptor genes were antenna-enriched. This study demonstrated a successful application of a transcriptome for discovering a large number of divergent chemoreceptor genes of a non-model organism. The findings provide a valuable sequence resource and gene tissue distribution information for systematic functional analysis of molecular mechanisms underlying chemoreception in this pest.
Assuntos
Perfilação da Expressão Gênica , Genes de Insetos , Receptores Odorantes/genética , Transcriptoma/genética , Gorgulhos/genética , Animais , Feminino , Ontologia Genética , Masculino , Anotação de Sequência Molecular , Especificidade de Órgãos/genética , Receptores Odorantes/metabolismo , Análise de Sequência de DNARESUMO
The aim of this study was to evaluate the control of blood glucose and glycosylated hemoglobin A1c (HbA1c) and its influencing factors, in elderly type 2 diabetic mellitus (T2DM) patients undergoing comprehensive management. After years of comprehensive prevention of and control measures for diabetes, elderly T2DM patients who were receiving long-term health care were comprehensively evaluated through an annual physical examination. In addition to routine health examination, the patients were required to undergo HbA1c measurement. Among 688 patients, 652 were men and 36 were women, with a mean age of 78.2 ± 9.1 years. The average HbA1c was 6.6 ± 0.9%. A total of 50.6% of the patients had HbA1c <6.5%, whereas 76.3% had HbA1c <7.0%. Among all patients, 77.1, 46.4, 66.1, 67.8, 36.3, and 57.4% achieved the target total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), blood pressure, and body mass index (BMI) levels, respectively. The duration of disease and type of treatment, as well as the LDL, HDL, TG, BMI, and blood pressure levels, were significantly associated with HbA1c control. No patient was admitted because of ketoacidosis or hyperosmolar nonketotic diabetic coma in 10 years. Approximately half of the T2DM patients achieved the target HbA1c level. The more effective blood glucose control observed in our study compared with previous studies can be attributed to the effective monitoring of medical conditions and comprehensive management of patients.
Assuntos
Doença de Alzheimer/sangue , Doença de Alzheimer/terapia , Glicemia/metabolismo , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/terapia , Idoso , Idoso de 80 Anos ou mais , Colesterol/sangue , Diabetes Mellitus Tipo 2/psicologia , Feminino , Hemoglobinas Glicadas/metabolismo , Humanos , MasculinoRESUMO
Hyperlipidemia is a well-established risk factor for the development of coronary atherosclerosis, while intermedin (IMD) has been identified as a novel calcitonin/calcitonin gene-related peptide family member involved in cardiovascular protection. However, whether IMD protects against hyperlipidemia-associated myocardial ischemia/reperfusion (MI/R) injury is unknown. We established a hyperlipidemia model using Sprague-Dawley rats, and created a MI/R condition by ligating the cardiac left circumflex artery. The possible pathophysiological role of IMD and its physiological function in MI/R was further studied. The level of IMD significantly decreased in hyperlipidemia rats (P < 0.05). After MI/R, the IMD level was increased both in the plasma and myocardial tissue of hyperlipidemia rats compared to the sham-operated rats (P < 0.001). As evaluated by the activity of LDH, CK-MB, MDA and SOD, additional IMD was revealed to alleviate MI/R heart injury in hyperlipidemia rats (P < 0.05). By regulating the process of cardiomyocyte apoptosis and inflammatory reaction, IMD could perform an important role in cardio-protection, especially against hyperlipidemia-associated MI/R injury. Additional IMD could protect cardiac myocytes against MI/R injury via reduction of apoptosis and inflammation in the hyperlipidemia rat model, and thus, it may play a potential role as a novel therapeutic target for cardiac ischemic injury in hyperlipidemic patients.