RESUMO
BACKGROUND: Colorectal cancer (CRC) is one of the most common malignant cancers in human, and its incidence increases gradually every year. Metastasis is an important factor leading to tumor development. The epithelial-mesenchymal transition (EMT) has been proved to be closely related to tumor metastasis, yet its related mechanism in CRC remains to be explored. METHODS: We obtained the differentially expressed gene C5aR1 with SETDB1 stable overexpression and knockdown cells by RNA-seq. Cell proliferation was tested by CCK8 and colony formation assay. Migration and invasion of CRC cells were determined by the wound healing and transwell invasion assay. The potential pathway of C5aR1 in CRC was preliminarily studied by western blotting. RESULTS: Sequencing results showed that C5aR1 was the most differentially expressed gene. By changing the expression of C5aR1 in CRC cells, this study found that C5aR1 promoted the proliferation, colony formation, migration and invasion of CRC cells in vitro. C5aR1 accelerated the EMT process and the expression of C5aR1 altered the molecular expression of key proteins in the Wnt/ß-catenin pathway. CONCLUSION: C5aR1 promotes the development of CRC and accelerates the EMT process. Furthermore, C5aR1 may involve in the regulation of Wnt/ß-catenin pathway in CRC.
Assuntos
Neoplasias Colorretais , Transição Epitelial-Mesenquimal , Receptor da Anafilatoxina C5a , Via de Sinalização Wnt , Humanos , beta Catenina/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células/genética , Neoplasias Colorretais/patologia , Transição Epitelial-Mesenquimal/genética , Regulação Neoplásica da Expressão Gênica , Receptor da Anafilatoxina C5a/genéticaRESUMO
Background: Lumpy jaw is disease effecting wallabies and kangaroos, particularly in Macropus rufus and Macropusgiganteus. In the most serious situations, additional tooth loss and fistulas follow, accompanied by a stench, weight loss,and eventually death due to sepsis or blood poisoning. Lumpy jaw disease has seriously affected the normal display andhealth of kangaroos, and cause a huge economic loss. There was an outbreak of jaw infection in kangaroos at the HongshanForest Zoo. Two Macropus giganteus and two Macropus rufus died of lumpy jaw. The main objective of the describingcase was to isolate pathogens, provide a basis for follow-up treatment, and serve to establish a disease prevention protocol.Case: Four grown-up kangaroos (two Macropus giganteus and two Macropus rufus) were raised in Hongshan Forest Zoo,which had obviously clinical symptoms, such as oral lesions of pus, necrotic tissue, rotting teeth, then died of lumpyjaw. Oral swab samples were collected from the lesion sites of the dying kangaroos. Mice experiments were conducted toexamine the pathogenicity of the strains. Tests of antimicrobial susceptibity were performed to prescribe with better drugtreatments for kangaroos. Corynebacterium pseudotuberculosis and Yersinia pseudotuberculosis were identified based onmorphology, culture characteristics and biochemical tests. Corynebacterium pseudotuberculosis (G+) in Sucrose, Mannitol,Lactose, Maltose, Glucose tubes were positive, that acids and gases both production, in Gelatin liquefaction, Indol test,MR were positive, that only acids production, others were negative; Yersinia pseudotuberculosis (G-) in Urea, MR werepositive, that only acids production, others were negative.The infected mice presented with gum erosion or ulcers whenthe two pathogens were injected subcutaneous at the oral regional by 2-3 point at 0.2 mL of individual strains 1.0×109CFU/mouse...(AU)
Assuntos
Animais , Macropodidae/microbiologia , Corynebacterium pseudotuberculosis/patogenicidade , Yersinia pseudotuberculosis/isolamento & purificação , Yersiniose/veterinária , Infecções por Corynebacterium/veterinária , Animais de Zoológico/microbiologia , Resistência a MedicamentosRESUMO
Chromoblastomycosis is a chronic fungal infection. Itraconazole and terbinaï¬ne are the most recommended antifungal drugs for chromoblastomycosis, while amphotericin B is not usually recommended. A patient with chromoblastomycosis in our hospital showed poor clinical responses to itraconazole and terbinaï¬ne. The fungus isolated from the lesions of this patient was identified as Fonsecaea nubica and numbered zssy0803. In vitro antifungal susceptibilities of F. nubica zssy0803 to terbinaï¬ne, amphotericin B, itraconazole, voriconazole and caspofungin were evaluated, as well as the combinations of terbinaï¬ne with the other four antifungals. The combined effect of terbinaï¬ne and amphotericin B on other 20 clinical F. nubica strains was also evaluated. The minimal inhibitory concentrations of terbinaï¬ne, amphotericin B, itraconazole, voriconazole and caspofungin on F. nubica zssy0803 were 0.25 µg/mL, 2 µg/mL, 1 µg/mL, 4 µg/mL and 8 µg/mL, respectively. The combination of terbinaï¬ne and amphotericin B showed the lowest fractional inhibitory concentration index of 0.28 to F. nubica zssy0803 in comparison with combinations of terbinaï¬ne and the other four antifungal drugs. The combination of terbinaï¬ne and amphotericin B was also synergistic for all the other 20 F. nubica strains. Then, the combination of oral terbinaï¬ne (500 mg/day) and intralesional injections of amphotericin B (1 mg/mL) was used to treat this patient. After this combined therapy for 25 weeks and terbinaï¬ne monotherapy for additional 12 weeks, the patient was cured. These findings indicate for the first time that terbinaï¬ne and amphotericin B are synergistic in killing F. nubica both in vitro and in vivo.
Assuntos
Anfotericina B/administração & dosagem , Antifúngicos/administração & dosagem , Ascomicetos/efeitos dos fármacos , Cromoblastomicose/tratamento farmacológico , Terbinafina/administração & dosagem , Ascomicetos/isolamento & purificação , Sinergismo Farmacológico , Quimioterapia Combinada , Feminino , Humanos , Testes de Sensibilidade Microbiana , Pessoa de Meia-IdadeRESUMO
Background: Lumpy jaw is disease effecting wallabies and kangaroos, particularly in Macropus rufus and Macropusgiganteus. In the most serious situations, additional tooth loss and fistulas follow, accompanied by a stench, weight loss,and eventually death due to sepsis or blood poisoning. Lumpy jaw disease has seriously affected the normal display andhealth of kangaroos, and cause a huge economic loss. There was an outbreak of jaw infection in kangaroos at the HongshanForest Zoo. Two Macropus giganteus and two Macropus rufus died of lumpy jaw. The main objective of the describingcase was to isolate pathogens, provide a basis for follow-up treatment, and serve to establish a disease prevention protocol.Case: Four grown-up kangaroos (two Macropus giganteus and two Macropus rufus) were raised in Hongshan Forest Zoo,which had obviously clinical symptoms, such as oral lesions of pus, necrotic tissue, rotting teeth, then died of lumpyjaw. Oral swab samples were collected from the lesion sites of the dying kangaroos. Mice experiments were conducted toexamine the pathogenicity of the strains. Tests of antimicrobial susceptibity were performed to prescribe with better drugtreatments for kangaroos. Corynebacterium pseudotuberculosis and Yersinia pseudotuberculosis were identified based onmorphology, culture characteristics and biochemical tests. Corynebacterium pseudotuberculosis (G+) in Sucrose, Mannitol,Lactose, Maltose, Glucose tubes were positive, that acids and gases both production, in Gelatin liquefaction, Indol test,MR were positive, that only acids production, others were negative; Yersinia pseudotuberculosis (G-) in Urea, MR werepositive, that only acids production, others were negative.The infected mice presented with gum erosion or ulcers whenthe two pathogens were injected subcutaneous at the oral regional by 2-3 point at 0.2 mL of individual strains 1.0×109CFU/mouse...
Assuntos
Animais , Corynebacterium pseudotuberculosis/patogenicidade , Infecções por Corynebacterium/veterinária , Macropodidae/microbiologia , Yersinia pseudotuberculosis/isolamento & purificação , Yersiniose/veterinária , Animais de Zoológico/microbiologia , Resistência a MedicamentosRESUMO
Cutaneous Rosai-Dorfman disease is a rare, lymphoproliferative disease. It is benign and self-limited, only involves skin and subcutaneous tissue and typically occurs as histiocyte-rich inflammatory infiltrates, manifesting as erythematous to brown papules, plaques, or nodules, without predilection for site. The authors describe a case of cutaneous Rosai-Dorfman disease in a 72-year-old man who presented erythematous and scaled plaque on the right neck for three months without systemic symptoms. Owing to local involvement, the patient received a surgery to exsect the lesion completely and remained asymptomatic with no signs of recurrence at the 9-month follow up.
Assuntos
Histiocitose Sinusal/patologia , Dermatopatias/patologia , Idoso , Histiocitose Sinusal/cirurgia , Humanos , Masculino , Pescoço/patologia , Pele/patologia , Dermatopatias/cirurgiaRESUMO
Cutaneous Rosai-Dorfman disease is a rare, lymphoproliferative disease. It is benign and self-limited, only involves skin and subcutaneous tissue and typically occurs as histiocyte-rich inflammatory infiltrates, manifesting as erythematous to brown papules, plaques, or nodules, without predilection for site. The authors describe a case of cutaneous Rosai-Dorfman disease in a 72-year-old man who presented erythematous and scaled plaque on the right neck for three months without systemic symptoms. Owing to local involvement, the patient received a surgery to exsect the lesion completely and remained asymptomatic with no signs of recurrence at the 9-month follow up.