RESUMO
OBJECTIVE: To explore the role of ADMA in gastric cancer. METHODS: The specimens of 115 gastric cancer patients were analyzed by ELISA and survival analysis. Functional assays were used to assess the effects of ADMA on gastric cancer cells. Experiments were conducted to detect the signaling pathway induced by ADMA in GC. RESULTS: Gastric cancer patients with high ADMA levels had poor prognosis and low survival rate. Furthermore, high level of ADMA did not affect the proliferation while promoted the migration and invasion of gastric cancer cell. Moreover, ADMA enhanced the epithelial-mesenchymal transition (EMT). Importantly, ADMA positively regulated ß-catenin expression in GC and promoted GC migration and invasion via Wnt/ß-catenin pathway. CONCLUSIONS: ADMA regulates gastric cancer cell migration and invasion via Wnt/ß-catenin signaling pathway and which may be applied to clinical practice as a diagnostic and prognostic biomarker.
Assuntos
Adenocarcinoma/sangue , Adenocarcinoma/patologia , Arginina/análogos & derivados , Transição Epitelial-Mesenquimal , Neoplasias Gástricas/sangue , Neoplasias Gástricas/patologia , Via de Sinalização Wnt , Adenocarcinoma/mortalidade , Arginina/sangue , Linhagem Celular Tumoral , Movimento Celular/fisiologia , Proliferação de Células , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/fisiopatologia , Prognóstico , Neoplasias Gástricas/mortalidade , Cicatrização/fisiologiaRESUMO
Chronic systemic inflammation and repetitive damage of vascular endothelia by incompatible dialysis system are probable causes of cardiovascular disease in patients on dialysis. The present study aimed to assess in vitro biocompatibility and anti-inflammatory effect of hemodialysis fluid supplemented with rosmarinic acid (RA) using human umbilical vein endothelial cells (HUVEC). HUVECs (5×106 cells/mL) were pre-exposed to 1 µg/mL of lipopolysaccharides (LPS) and incubated with RA-supplemented hemodialysis fluid (HDF). Cytotoxicity was assessed qualitatively by morphologic assessment and quantitatively by MTT assay. Expressions of proinflammatory mediators were assessed using quantitative real-time PCR and production of NO was quantified. Phosphorylation of AKT and nuclear localization of nuclear factor kappa B (NF-κB) were examined using western blotting. Exposure of HUVECs to RA-supplemented HDF had no influence on morphology and viability. Inhibition of proinflammatory mediator production in HUVECs by RA supplementation to HDF was significant in a dose-dependent manner. Exposure to RA-supplemented HDF resulted in a decrease in nitric oxide synthase expression and reduction of NO production in LPS-stimulated HUVECs. RA supplementation of HDF suppressed Akt activation in LPS-stimulated HUVECs. In addition, the level of cellular IκB was increased in parallel to a reduced nuclear translocation of NF-κB in LPS-induced endothelial cells. Our results suggest that RA-supplemented HDF is biocompatible and significantly suppressed inflammation induced in endothelial cells. In this respect, the use of HDF supplemented with RA could alleviate inflammation and improve long-term treatment of patients with renal failure on dialysis. Further clinical studies are required to confirm the effects.
Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Materiais Biocompatíveis/farmacologia , Cinamatos/farmacologia , Depsídeos/farmacologia , Soluções para Hemodiálise/farmacologia , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Inflamação/tratamento farmacológico , Análise de Variância , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Citocinas/análise , Citocinas/efeitos dos fármacos , Formazans , Soluções para Hemodiálise/química , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Immunoblotting , Inflamação/metabolismo , Lipopolissacarídeos , NF-kappa B/análise , Óxido Nítrico/análise , Fosforilação , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes , Sais de Tetrazólio , Ácido RosmarínicoRESUMO
During endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), a needle is commonly used with a stylet, although recently the stylet has been omitted. This prospective study aimed to compare the quality of specimens obtained by EBUS-TBNA performed with and without a stylet. Between November 2013 and November 2014, 131 patients with lung cancer underwent EBUS-TBNA, with a total of 148 mediastinal or hilar lymph nodes sampled both with and without an inner-stylet, yielding 296 cytological specimens. Specimens were scored cytologically using five parameters: background blood or clot, amount of cellular material, degree of cellular degeneration, degree of cellular trauma, and retention of appropriate architecture. The procedure with a stylet required significantly longer operation time than without a stylet (14.5±0.8 vs 12.7±1.1 min, P<0.001). Excellent specimens were obtained in 261/296 and 260/296 samples in the procedures with and without a stylet, respectively (P=0.9), while the remaining 35 and 36 samples, respectively, were adequate. The diagnosing and staging of lung cancer using EBUS-TBNA did not differ significantly between the groups. In conclusion, specimen collection by EBUS-TBNA without a stylet is easier and faster than the procedure using a stylet and absence of a stylet did not alter specimen quality or diagnostic accuracy.
Assuntos
Aspiração por Agulha Fina Guiada por Ultrassom Endoscópico , Neoplasias Pulmonares/patologia , Linfonodos/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Aspiração por Agulha Fina Guiada por Ultrassom Endoscópico/instrumentação , Aspiração por Agulha Fina Guiada por Ultrassom Endoscópico/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Estudos ProspectivosRESUMO
During endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA), a needle is commonly used with a stylet, although recently the stylet has been omitted. This prospective study aimed to compare the quality of specimens obtained by EBUS-TBNA performed with and without a stylet. Between November 2013 and November 2014, 131 patients with lung cancer underwent EBUS-TBNA, with a total of 148 mediastinal or hilar lymph nodes sampled both with and without an inner-stylet, yielding 296 cytological specimens. Specimens were scored cytologically using five parameters: background blood or clot, amount of cellular material, degree of cellular degeneration, degree of cellular trauma, and retention of appropriate architecture. The procedure with a stylet required significantly longer operation time than without a stylet (14.5±0.8 vs 12.7±1.1 min, P<0.001). Excellent specimens were obtained in 261/296 and 260/296 samples in the procedures with and without a stylet, respectively (P=0.9), while the remaining 35 and 36 samples, respectively, were adequate. The diagnosing and staging of lung cancer using EBUS-TBNA did not differ significantly between the groups. In conclusion, specimen collection by EBUS-TBNA without a stylet is easier and faster than the procedure using a stylet and absence of a stylet did not alter specimen quality or diagnostic accuracy.
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Aspiração por Agulha Fina Guiada por Ultrassom Endoscópico , Neoplasias Pulmonares/patologia , Linfonodos/patologia , Aspiração por Agulha Fina Guiada por Ultrassom Endoscópico/instrumentação , Aspiração por Agulha Fina Guiada por Ultrassom Endoscópico/métodos , Estadiamento de Neoplasias , Estudos ProspectivosRESUMO
Quercetin, a dietary flavonoid abundant in fruits, vegetables, and herbs, presents various pharmacological effects. This study aimed to investigate the anti-inflammatory effect and the underlying mechanism of quercetin in lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMCs). Cell viability was measured by the Cell Counting Kit-8 assay. The mRNA expression of Toll-like receptor 2 (TLR2) was assessed by quantitative real-time polymerase chain reaction. Inflammatory cytokine secretions and nuclear factor (NF)-kB levels were analyzed by enzyme-linked immunosorbent assay. Our findings showed that quercetin significantly reduced LPS-induced cytotoxicity in human PBMCs. Quercetin suppressed the secretion of tumor necrosis factor-a, interleukin (IL)-1b, and IL-6 in LPS-stimulated human PBMCs. Moreover, quercetin reduced the LPS-induced increase in the expression of TLR2 mRNA and decreased the NF-kB concentration in LPS-stimulated human PBMCs. The data indicates that quercetin plays an important role in LPS-induced inflammation in human PBMCs via suppression of the TLR2-NF-kB pathway.
Assuntos
Inflamação/tratamento farmacológico , Leucócitos Mononucleares/efeitos dos fármacos , NF-kappa B/antagonistas & inibidores , Quercetina/farmacologia , Receptor 2 Toll-Like/antagonistas & inibidores , Anti-Inflamatórios/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Humanos , Inflamação/sangue , Interleucina-1beta/genética , Interleucina-6/genética , Leucócitos Mononucleares/imunologia , Lipopolissacarídeos/farmacologia , NF-kappa B/sangue , Transdução de Sinais/efeitos dos fármacos , Receptor 2 Toll-Like/sangue , Fator de Necrose Tumoral alfa/genéticaRESUMO
The goal of the current study was to investigate the effects of pollution on aquatic organisms in the Yongcheng coal mine subsidence area. Crucian carp (Carassius auratus) were collected from Yongcheng natural fishpond (experimental group) and Tianmu Lake (control group), and the spleens were isolated for analysis. Subsequently, histological changes, DNA damage, and antioxidant enzyme activity were assessed. The result showed that there were more vacuoles, widened blood sinus cavities, increased partial dot necrosis, and a larger number of brown-yellow nodules in splenic sections stained with hematoxylin and eosin in the experimental group than in the control group. Additionally, it was not easy to distinguish red pulp from white pulp in the experimental group. The antioxidant enzyme activity in the experimental group was significantly lower than that in the control group (P < 0.01). Comet assay results showed varying degrees of tailing and DNA chain breaks in the experimental group, and further analysis demonstrated that the tail length and tail moment were significantly increased compared to those in the control group (P < 0.01). These results suggest that the spleen antioxidant defense system was severely damaged in crucian carp from the Yongcheng coal mine subsidence area.
Assuntos
Minas de Carvão , Proteínas de Peixes/metabolismo , Peroxidases/metabolismo , Baço/efeitos dos fármacos , Poluição da Água/efeitos adversos , Animais , Carpas , China , Dano ao DNA , Necrose , Baço/enzimologia , Baço/patologiaRESUMO
Systemic lupus erythematosus (SLE) is an autoimmune connective tissue disease that affects multiple organs and diminishes a patients' quality of life. It has been suggested that interleukin 19 (IL-19) is engaged in intercellular signal transduction, which is related to the immune response and the local inflammatory reaction. Single nucleotide polymorphisms (SNPs) have been used to explore the genetic basis underlying the pathogenesis of SLE. In this study, we investigated the potential correlation between the functional IL19 SNP rs2243188 and SLE. The frequency of allele C in rs2243188 was lower in the SLE population, particularly when the dominant inheritance model was applied. There was also a significant difference in the allele C frequency between the lupus nephritis (LN) and non-LN groups in both the dominant and recessive inheritance models. In addition, we identified significant differences in the serum IL-19 levels between the different classes of SLE. Although this study is still at the preliminary stage, the correlations between the IL19 SNP and SLE, and between the IL-19 levels and the different subclasses of SLE provide a reference for further exploration.
Assuntos
Interleucinas/genética , Nefrite Lúpica/genética , Polimorfismo de Nucleotídeo Único , Adolescente , Adulto , Estudos de Casos e Controles , Feminino , Frequência do Gene , Humanos , Interleucinas/sangue , Nefrite Lúpica/sangue , Masculino , Pessoa de Meia-IdadeRESUMO
In this study, the effect of host density, host, and parasitoid ages in choice and no-choice tests on the parasitism performance of Tetrastichus brontispae Ferriere, one of the major parasitoid of Brontispa longissima (Gestro), was investigated in the laboratory. The results revealed that an increased host density resulted in no increased parasitism of B. longissima by T. brontispae; the optimal host density was three host pupae per parasitoid when considering the costs for mass rearing. Moreover, parasitoid age was quite crucial for effective parasitism and affected the emergence rate. Although 2-h to 4-day-old parasitoids successfully parasitized the host pupae, younger parasitoids (within 2-day-old) presented higher parasitism capacity than older parasitoids. More importantly, both choice and no-choice tests confirmed that all host stages tested from 2-h to 4-day-old were suitable for T. brontispae parasitization, although 2-h to 2-day-old hosts were preferred. We also demonstrated that sex ratio, emergence rate, and egg to adult developmental time were not influenced by host density, parasitoid, and host age in both choice and no-choice tests. Our data will allow for more accurate prediction and interpretation on the parasitization by T. brontispae, supporting mass-production initiatives and mass release in programs of B. longissima.
Assuntos
Besouros/parasitologia , Himenópteros/patogenicidade , Animais , Interações Hospedeiro-Parasita , Pupa , VespasRESUMO
Calcium plays a critical role in regulating abiotic stress responses in plants. Calcineurin B-like (CBL) proteins are calcium sensors in calcium signaling pathways. However, the molecular mechanisms underlying calcium signaling remain to be elucidated. In this study, the CBL1 gene, which codes for the CBL protein, was isolated from the birch-leaf pear. One 2,969-bp sequence was cloned using PCR, and using the cloned 2,027-bp sequence was isolated from pear genomic DNA via genome walking. Sequencing analysis revealed that the 4,996-bp sequence was a PbCBL1 gene consisting of eight exons and seven introns, and the 2,027-bp sequence was identified as the promoter of the PbCBL1 gene, which contains the basic promoter elements TATA and CAAT boxes. In addition, some other cis-acting elements including heat, cold, drought, and hormone responsive elements were also present. To further investigate the activity of this promoter, the sequence was used to drive a GUS fusion gene into leaf discs of tobacco (Nicotiana benthamiana) with Agrobacterium-mediated transformation method. GUS gene expression could be regulated by the PbCBL1 promoter following induction by GA, ABA, SA, and MeJA. Furthermore, the results of real-time RT-qPCR indicate that the PbCBL1 gene can respond to changes in the intracellular calcium concentration, and that it can be induced by cold, heat, drought, and stress by several hormones including GA, ABA, SA, and MeJA. PbCBL1 gene may be involved in several signal transduction pathways, and play an important role in the condition of adversity stress in pear.
Assuntos
Proteínas de Ligação ao Cálcio/genética , Clonagem Molecular , Proteínas de Plantas/genética , Regiões Promotoras Genéticas , Pyrus/genética , Região 5'-Flanqueadora , Sequência de Aminoácidos , Sequência de Bases , Expressão Gênica , Ordem dos Genes , Genes Reporter , Vetores Genéticos , Dados de Sequência Molecular , Motivos de Nucleotídeos , Proteínas Recombinantes de Fusão , Sequências Reguladoras de Ácido Nucleico , Reprodutibilidade dos Testes , Alinhamento de Sequência , Análise de Sequência de DNA , Transformação GenéticaRESUMO
SWEETs are a recently discovered class of sugar transporters that mediate glucose uptake in the intestine and mammary glands. Our objectives were to clone goat SWEET1 and conduct a functional analysis of its effect on glucose efflux in goat mammary gland epithelial cells. We cloned and sequenced the goat SWEET1 gene from goat mammary glands, then conducted an analysis of the structure of goat SWEET1, including a prediction of the transmembrane helices and potential N-glycosylation sites. To investigate the biological function of goat SWEET1, we also generated goat SWEET1-transfected goat mammary gland epithelial cells using the eukaryotic expression vector pcDNA3.1-gSWEET1. Goat SWEET1 overexpression can reduce glucose absorption in mammary gland epithelial cells with increasing expression of GLUT1, GLUT4, and GLUT12, which may be attributed to glucose efflux arising from the leading role played by goat SWEET1. This study will improve our understanding of the glucose balance in mammary glands and the level of glucose in milk.