RESUMO
The hydrolysis of BANA by subgingival plaque samples is associated with the presence of either Treponema denticola, Porphyromonas gingivalis, and/or Bacteroides forsythus. A protocol in which pure cultures were incubated for 15 min at 55 degrees C detected about 5 x 10(5) CFU of P. gingivalis and 1 x 10(6) CFU of T. denticola. Clinical studies indicated that the BANA test in this configuration will detect about 10(4) organisms in vivo as compared with the 10(5) to 10(6) organisms found with in vitro grown cells. The BANA test can be made less sensitive by decreasing the time and/or temperature of incubation, which could improve the specificity of the test. In the present study we determined the incubation parameters that would give optimal specificity when the plaque samples were removed from sites of gingival health. Twenty-six approximal plaque samples were taken from each of 90 clinically healthy subjects and incubated with the BANA substrate on PerioScan cards (Oral-B Laboratories) for 5 and 15 min at 35 degrees, 45 degrees, and 55 degrees C. Subjects were randomly assigned to the various temperatures. Wooden toothpicks were inserted interproximally in all sites anterior to distal of the first molars and then each side of the toothpick was wiped onto the PerioScan card. The specificity of the BANA test relative to clinical health was 96% when the cards were incubated for 5 min at 35 degrees C, but decreased to 50-70% when the cards were incubated for 15 min at 35 degrees C or for 5 and 15 min at 45 degrees C and 55 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Benzoilarginina-2-Naftilamida , Ensaios Enzimáticos Clínicos , Cisteína Endopeptidases , Doenças Periodontais/diagnóstico , Porphyromonas gingivalis/isolamento & purificação , Treponema/isolamento & purificação , Adulto , Catepsina H , Catepsinas/análise , Contagem de Colônia Microbiana , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Doenças Periodontais/microbiologia , Porphyromonas gingivalis/enzimologia , Sensibilidade e Especificidade , Temperatura , Fatores de Tempo , Treponema/enzimologiaRESUMO
Caries examination and collection of paraffin wax-stimulated saliva samples were performed in 37 children, 3-6 years old, in a child-care facility at the Vidigal slum, Rio de Janeiro, Brazil. The levels of mutans streptococci and lactobacilli in saliva were estimated by the Cariescreen and by the Dentocult tests and the saliva secretion rate was determined. Statistical analysis was performed on surface-based and patient-based caries prevalence rates (SBCPR and PBCPR), and related to bacterial and salivary parameters. The results show that 31 of the 37 children were caries active. The SBCPR for the primary dentition was 6.7% +/- 1.0%. Occlusal surfaces were the most affected by decay. Regression analysis revealed that mutans streptococci salivary levels were significantly associated with the SBCPR (P = 0.0001). Similarly, lactobacilli salivary levels were significantly associated with the SBCPR (P = 0.0001). No significant association could be found between the saliva secretion rate and the SBCPR. When regression analysis was used to model dependence of the SBCPR on both organisms, the mutans streptococci and lactobacilli salivary levels were significantly associated with the SBCPRs (P = 0.0021 and 0.0118, respectively), and salivary levels of these organisms accounted for 57% of the SBCPR variability. These findings indicate that the levels of mutans streptococci and lactobacilli in saliva are significantly related to the SBCPRs on the primary dentition of these children.