RESUMO
Burkholderia glumae causes bacterial leaf blight in rice, and its global spread has been exacerbated by climate change. To understand the genetic diversity and virulence of B. glumae strains isolated from rice cultivars in Perú, 47 isolates were obtained from infected rice fields, all belonging to B. glumae, and confirmed by recA and toxB sequences. The BOX-PCR typing group has 38 genomic profiles, and these turn into seven variable number tandem repeats (VNTR) haplotypes. There was no correlation between clustering and geographical origin. Nineteen strains were selected for phenotypic characterization and virulence, using both the maceration level of the onion bulb proxy and inoculation of seeds of two rice cultivars. Several strains produced pigments other than toxoflavin, which correlated with onion bulb maceration. In terms of virulence at the seed level, all strains produced inhibition at the root and coleoptile level, but the severity of symptoms varied significantly between strains, revealing significant differences in pathogenicity. There is no correlation between maceration and virulence scores, probably reflecting different virulence mechanisms depending on the host infection stage. This is the first study to evaluate the VNTR diversity and virulence of Peruvian strains of B. glumae in two commercial cultivars.
Assuntos
Burkholderia , Variação Genética , Oryza , Doenças das Plantas , Oryza/microbiologia , Burkholderia/genética , Burkholderia/patogenicidade , Burkholderia/isolamento & purificação , Doenças das Plantas/microbiologia , Virulência/genética , Filogenia , Repetições MinissatélitesRESUMO
CASE: We present a case of a 70-year-old woman with simultaneous periprosthetic joint infection (PJI) of both hips and left knee due to a bilateral psoas abscess. The patient underwent debridement and implants removal with the consequent reimplantation in a sequential six-stage revision surgery. At four years of follow-up and in spite of the patient's comorbidities and current PJI presentation, she maintains full activities of daily living without restrictions. CONCLUSION: Accurate and early diagnosis of a psoas abscess is crucial. This case report provides experience of a complex scenario, the decision-making involved and the outcomes of an underdiagnosed complication.
Assuntos
Artroplastia de Quadril/efeitos adversos , Artroplastia do Joelho/efeitos adversos , Diagnóstico Tardio , Infecções Relacionadas à Prótese/etiologia , Infecções Relacionadas à Prótese/cirurgia , Abscesso do Psoas/complicações , Abscesso do Psoas/diagnóstico , Reoperação/métodos , Infecções Estafilocócicas/complicações , Infecções Estafilocócicas/diagnóstico , Staphylococcus aureus , Idoso , Feminino , HumanosRESUMO
This study investigated the molecular characteristics of six blaKPC-positive Enterobacteriaceae recovered from three patients in Argentina. Antimicrobial susceptibility testing was performed following Clinical and Laboratory Standards Institute (CLSI) 2014 recommendations. Molecular characterisation of the isolates was performed by biparental conjugation, PCR, sequencing, S1 nuclease restriction, and Southern blot hybridisation with a blaKPC probe using standard protocols and conditions. The isolates studied were as follows. Case 1: Escherichia coli (ECO-P1) and Klebsiella pneumoniae (KPN-P1) isolated from a rectal swab harboured blaKPC-2 in transposon Tn4401a on non-typeable and non-conjugative plasmids. Case 2: Enterobacter cloacae (ECL-P2) and K. pneumoniae (KPN-P2) were isolated from two blood cultures. blaKPC-2 was found in a novel genetic variant of ISKpn8-blaKPC-2-ISKpn6-like on conjugative plasmids of IncL/M type. Case 3, Citrobacter freundii (CFR-P3) and Klebsiella oxytoca (KOX-P3) were isolated from skin and skin-structure infection. The blaKPC gene was detected on ISKpn8-ΔblaTEM-blaKPC-2-ISKpn6-like located on an IncA/C conjugative plasmid. CFR-P3 and KOX-P3 harboured blaPER-2 in addition to the blaKPC gene. In conclusion, we document the horizontal dissemination of blaKPC-2 from diverse Enterobacteriaceae clinical isolates with different genetic backgrounds. This is the first report of E. coli harbouring blaKPC associated with Tn4401a in Argentina.
RESUMO
The present work describes the abrupt emergence of Klebsiella pneumoniae carbapenemase (KPC) and characterizes the first 79 KPC-producing enterobacteria from Argentina (isolated from 2006 to 2010). The emergence of bla(KPC-2) was characterized by two patterns of dispersion: the first was the sporadic occurrence in diverse enterobacteria from distant geographical regions, harbouring plasmids of different incompatibility groups and bla(KPC-2) in an unusual genetic environment flanked by ISKpn8-Δbla(TEM-1) and ISKpn6-like. bla(KPC-2) was associated with IncL/M transferable plasmids; the second was the abrupt clonal spread of K. pneumoniae ST258 harbouring bla(KPC-2) in Tn4401a.
Assuntos
Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/genética , beta-Lactamases/genética , Argentina/epidemiologia , Técnicas de Tipagem Bacteriana , Conjugação Genética , Elementos de DNA Transponíveis , Enterobacter/genética , Genes Bacterianos , Hospitais , Humanos , Infecções por Klebsiella/microbiologia , Infecções por Klebsiella/transmissão , Klebsiella pneumoniae/classificação , Klebsiella pneumoniae/enzimologia , Klebsiella pneumoniae/isolamento & purificação , Tipagem de Sequências Multilocus , Plasmídeos/genéticaRESUMO
Se presenta la experiencia acumulada en cirugía laparoscópica ginecológica en el Hospital Clínico Regional Valdivia. Se analizaron los procedimientos efectuados, las complicaciones observadas y los resultados en términos de embarazo en la mujer infértil. Se concluye que por esta vía puede resolverse la mayor parte de la cirugía anexial y la de la mujer infértil, con una baja incidencia de complicaciones; pero los resultados en términos reproductivos, son dependientes de la patología tubo-peritoneal
Assuntos
Humanos , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Doenças dos Anexos/cirurgia , Infertilidade Feminina/cirurgia , Laparoscopia/estatística & dados numéricos , Procedimentos Cirúrgicos em Ginecologia/estatística & dados numéricos , Salpingostomia/estatística & dados numéricos , Aderências Teciduais/cirurgiaRESUMO
Se reporta el caso clínico de una mujer con embarazo de 5 semanas quien se autoadministra una dosis aproximada de 11.200 mcg de misoprostol, (prostaglandina análoga E1 usada para el tratamiento preventivo de la úlcera gástrica) equivalente a 2 frascos de 28 comprimidos de 200 mcg cada uno, por vía intravaginal con fines abortivos, quien presenta manifestaciones tóxicas características por diarrea, vómitos hemáticos, shock, síndrome hemorragíparo y coma, que la conducen a la muerte en un período de 11 horas. Se discute y analizan los hallazgos clínicos en base a la literatura existente