RESUMO

Ten captive neotropical Brazilian feline were submitted to gastroscopic examination and samples of gastric mucosa from fundus, corpus and pyloric antrum were evaluated for the presence of Helicobacter species. Warthin-Starry (WS) staining and PCR assay with species-specific primers and enzymatic cleavage were applied for bacterial detection and identification. Histological lesions were evaluated by haematoxylin and eosin staining. All animals showed normal gross aspect of gastric mucosa. Helicobacter heilmannii was confirmed in 100% of the samples by WS and PCR assay. Mild lymphocytic infiltrate in the lamina propria was observed in eight animals, mainly in the fundus region. Small lymphoid follicles were seen in three animals. No significant association between Helicobacter infection and histological findings was verified. These observations suggest that gastric Helicobacter spp. could be a commensal or a eventual pathogen to captive neotropical feline, and that procedures, way life, and stress level on the shelter apparently had no negative repercussion over the integrity of the stomach.

RESUMO

Ten captive neotropical Brazilian feline were submitted to gastroscopic examination and samples of gastric mucosa from fundus, corpus and pyloric antrum were evaluated for the presence of Helicobacter species. Warthin-Starry (WS) staining and PCR assay with species-specific primers and enzymatic cleavage were applied for bacterial detection and identification. Histological lesions were evaluated by haematoxylin and eosin staining. All animals showed normal gross aspect of gastric mucosa. Helicobacter heilmannii was confirmed in 100% of the samples by WS and PCR assay. Mild lymphocytic infiltrate in the lamina propria was observed in eight animals, mainly in the fundus region. Small lymphoid follicles were seen in three animals. No significant association between Helicobacter infection and histological findings was verified. These observations suggest that gastric Helicobacter spp. could be a commensal or a eventual pathogen to captive neotropical feline, and that procedures, way life, and stress level on the shelter apparently had no negative repercussion over the integrity of the stomach.

RESUMO

One hundred and fifty fecal samples from dogs with acute diarrhea (Group 1) and from 50 dogs without diarrhea (Group 2) were collected and analyzed at the Veterinary Teaching Hospital of the State University of Londrina, between October, 2004 and October 2005. From the positive samples (29.3%) of Group 1, 72.7% were infected by only one agent, while 27.3% had mixed infection. The following genera were observed by coproparasitologic evaluation: Giardia sp. (9.3%), Ancylostoma sp. (7.3%), Cystoisospora (7.3%), Entamoeba sp. (6.7%), Toxocara sp.(4%), Pentatrichomanas hominis (3.3%), and Trichuris vulpis (1.3%). From the 50 animals without diarrhea, six (12%) were positive by coproparasitologic evaluation: two (4%) for Ancylostoma sp., three (6%) with Giardia sp., and one (2%) for both Ancylostoma sp. and Cystoisospora sp. There was a significant relationship between infection and diarrhea (P = 0,005), age and infection (P 0,001); but not between the occurrence of gastrointestinal parasites in males and females (P = 0.08). The number of infected pure breed dogs was significantly higher than their mixed breed counterparts (P = 0.01). The results show the importance of realizing periodic parasitological examination in dogs with or without diarrhea for the specific treatment and the implementation of prophylaxis and control methods.  

Foram examinadas 150 amostras de fezes colhidas de cães com diarréia aguda (Grupo 1), e outras 50 de animais sem diarréia (Grupo 2), atendidos entre outubro de 2004 e outubro 2005, no Hospital Veterinário Escola da Universidade Estadual de Londrina. Dos 150 animais do G1, 44 (29,3%) estavam infectados por helmintos e/ou protozoários, sendo que destes, 32 (72,7%) apresentavam infecção simples e 12 (27,3%) infecções múltiplas. Os gêneros identificados nestes animais foram: Giardia sp. (9,3%), Cystoisospora sp. (7,3%), Ancylostoma sp. (7,3%), Entamoeba sp. (6,7%), Toxocara sp. (4%), Pentatrichomonas hominis (3,3%) e Trichuris vulpis (1,3%). Dos 50 animais sem diarréia, seis (12%) eram positivos, dois (4%) para Ancylostoma sp., três (6%) para Giardia sp. e um (2%) para Ancylostoma sp. e Cystoisospora simultaneamente. Houve relação estatisticamente significativa (P=0,005) entre o parasitismo e a ocorrência de diarréia, assim como entre a faixa etária e a proporção de infecções (p 0,001), porém, machos e fêmeas foram igualmente acometidos (P=0,08). O número de cães com raça definida foi significativamente maior do que de cães sem definição racial (P = 0,01). Os resultados demonstram a importância de realização periódica de exames parasitológicos em cães com e sem diarréia para tratamento específico e adoção medidas de controle e profilaxia.  

RESUMO

Hemorrhagic gastroenteritis (GHE) is a common motivation to hospitalization and mortality between puppies. Considering the beneficial effects of probiotics in the treatment of some gastrointestinal disorders, we evaluated the usefulness of the probiotic product in 100 puppies hospitalized because GHE. Fifth dogs were treated by intravenous fluid, antiemetic, antibiotics and probiotic product (G1), and the other 50 only by supportive and symptomatic therapy (G2). We accessed the titters of anticorpus anti-parvovirus by HI, fecal excretion of virus by HA and registered the duration of internation period and tolerance to probiotic ingestion. The titters of HI were similar between G1 and G2 in the samples from entrance (P = 0,553) and at the hospital discharge (P = 0,844), moreover arises during hospitalization in booth groups (P 0,001). The fecal excretion of virus of G1 and G2 were similar in booth moments (P = 0,746 and P = 0,294, respectively) furthermore the G1 showed lowest fecal titters of virus at hospital discharge (P 0,001). The internation period in the G1 variates one to 15 days and one to 10 days in the G2 group, without statistical difference between the groups (P = 0,70). The rate of mortality in the G1 was 37,50% (18/48) and 26% (13/50) in the G2, lacking statistical significance (P = 0,49). Probiotic tolerance was regular to excellent in 95% of the admini

A gastrenterite hemorrágica (GHE) é uma afecção comum em filhotes de cães, e motivo freqüente de internação e mortalidade. Considerando que probióticos têm sido apontados como benéficos no tratamento destes pacientes, avaliamos o impacto da inclusão de probiótico à base de Lactobacilus acidophillus em 100 filhotes de cães com GHE, distribuídos em dois grupos de 50 indivíduos. Os cães do grupo 1 (G1), além da terapia indicada para GEH, constituída de fluido e antibiótico terapia e antiemético, receberam, por via oral, probiótico. Para os animais do grupo 2 (G2) foi adotada apenas a terapêutica convencional. Foram quantificadas as partículas virais nas fezes, pela aglutinação (HA) e os anticorpos (Ac) anti-parvovírus por inibição da hemaglutinação. Foram registradas a duração da internação e a tolerância dos animais ao probiótico. A excreção viral nas fezes pelos animais de ambos os grupos foi semelhante no momento da internação (P = 0,746) e da alta hospitalar (P = 0,294). Entretanto, no G1 a excreção foi significativamente menor no momento da alta (p 0,001). O período de internamento variou de um a 15 dias no G1 e de um a 10 dias no G2 (P = 0,70). A taxa de mortalidade no G1 foi de 37,50% (18/48) e de 26% (13/50) no G2 (P = 0,49). A aceitação do probiótico variou de regular a ótima em 95% das administrações, porém, os animais que foram a óbito demonstraram intolerância. Co

RESUMO

Intramedullary infusion technique was evaluated in 31 dogs less than three months of age, weighing 920 to 3,385 grams and showing no clinical signs of disease. They were randomly divided into group 1 (G1) and group 2 (G2) of 15 and 16 dogs, respectively. G1 received 0.9% NaCI solution and G2 received whole blood. The time necessary to perform intramedullary needle placement, maximum intramedullary infusion rate and any other event observed during the infusion period were recorded. The dogs were clinically evaluated on a daily basis during 30 days and radiographies were taken on days 15 and 30 after the procedure. Intramedullary infusion technique was easilyaccomplished and rapidly performed. Our results show that intramedullary route is safe and may be of great value as an alternative on the treatment of young dogs with critical hemodynamic alterations when the intravenous route is not accessible.

0 uso da via intramedular foi avaliado em 31 cães clinicamente normais, de até três meses de idade e com peso entre 920 e 3.385 g, divididos, aleatoriamente, em grupo 1 (G1), com 15 animais, e grupo 2 (G2), com 16. Aqueles do G1 foram tratados com NaCI a 0,9% e os do G2 com sangue total. Foram anotados os tempos gastos para implantação da agulha, as velocidades máximas de infusão obtidas e as ocorrências havidas durante o período de infusão. Todos os animais foram avaliados clinicamente durante os 30 dias de experimentação e por meio de radiografias nos dias 15 e 30 do experimento. Constatou-se que a técnica é de fácil e rápida execução e pouco cruenta. Os resultados observados indicam que a via intramedular é segura, sendo uma alternativa valiosa à via intravenosa no tratamento de filhotes de cães com graves alterações hemodinâmicas.