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1.
Clin Exp Dermatol ; 38(5): 470-7, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23678890

RESUMO

BACKGROUND: Both leprosy and human immunodeficiency virus (HIV) are infectious diseases, and are an important global health problem. Patients with leprosy who are co-infected with HIV seem to be at higher risk of developing leprosy reactions. AIM: To examine the histological features of leprosy in patients with HIV and leprosy co-infection, particularly to determine whether the typical leprosy histopathology is present in skin biopsies, and to assess the histological features of leprosy reactions in co-infected patients. METHODS: This was a matched cohort study with 11 co-infected patients and 31 HIV-negative patients with leprosy. A structured protocol for skin-biopsy evaluation was followed, focusing on inflammation of the skin and dermal nerves. RESULTS: Of the 11 HIV-positive patients, 7 (63%) had borderline tuberculoid (BT) leprosy and 5 (70%) of these 7 patients had developed a type 1 reaction. The lesions in these patients were immunologically active, with 100% of biopsies having evidence of compact granulomas, 90% evidence of oedema and 30% evidence of necrosis. CONCLUSIONS: In this study, patients co-infected with HIV and M. leprae had the typical histological lesions of leprosy. There was evidence of immune activation in patients who received combination antiretroviral therapy, and these patients had BT leprosy and leprosy-upgrading reactions.


Assuntos
Coinfecção/patologia , Infecções por HIV , Hanseníase/patologia , Adulto , Idoso , Brasil , Contagem de Linfócito CD4 , Estudos de Coortes , Coinfecção/imunologia , Coinfecção/virologia , Feminino , Infecções por HIV/imunologia , Humanos , Hanseníase/imunologia , Hanseníase/virologia , Masculino , Pessoa de Meia-Idade , Adulto Jovem
2.
s.l; s.n; 2013. 8 p. ilus, tab.
Não convencional em Inglês | Sec. Est. Saúde SP, HANSEN, Hanseníase, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1095725

RESUMO

BACKGROUND: Both leprosy and human immunodeficiency virus (HIV) are infectious diseases, and are an important global health problem. Patients with leprosy who are co-infected with HIV seem to be at higher risk of developing leprosy reactions. AIM: To examine the histological features of leprosy in patients with HIV and leprosy co-infection, particularly to determine whether the typical leprosy histopathology is present in skin biopsies, and to assess the histological features of leprosy reactions in co-infected patients. METHODS: This was a matched cohort study with 11 co-infected patients and 31 HIV-negative patients with leprosy. A structured protocol for skin-biopsy evaluation was followed, focusing on inflammation of the skin and dermal nerves. RESULTS: Of the 11 HIV-positive patients, 7 (63%) had borderline tuberculoid (BT) leprosy and 5 (70%) of these 7 patients had developed a type 1 reaction. The lesions in these patients were immunologically active, with 100% of biopsies having evidence of compact granulomas, 90% evidence of oedema and 30% evidence of necrosis. CONCLUSIONS: In this study, patients co-infected with HIV and M. leprae had the typical histological lesions of leprosy. There was evidence of immune activation in patients who received combination antiretroviral therapy, and these patients had BT leprosy and leprosy-upgrading reactions.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Brasil , Infecções por HIV , Infecções por HIV/imunologia , Estudos de Coortes , Contagem de Linfócito CD4 , Coinfecção/imunologia , Coinfecção/patologia , Coinfecção/virologia , Hanseníase/imunologia , Hanseníase/patologia , Hanseníase/virologia
3.
Rev Inst Med Trop Sao Paulo ; 41(4): 239-42, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10564918

RESUMO

The authors studied 70 leprosy patients and 20 normal individuals, comparing the traditional sera collection method and the finger prick blood with the conservation on filter paper for specific antibodies against the native phenolic glycolipid-I (PGL-I) from Mycobacterium leprae. The finger prick blood dried on filter paper was eluated in phosphate buffer saline (PBS) containing 0.5% gelatin. The classical method for native PGL-I was performed for these eluates, and compared with the antibody determination for sera. It was observed that there is a straight correlation comparing these two methods; although the titles found for the eluates were lower than those obtained for serology. This blood collection method could be useful for investigation of new leprosy cases in field, specially in contacts individuals.


Assuntos
Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/imunologia , Coleta de Amostras Sanguíneas/métodos , Glicolipídeos/sangue , Hanseníase/imunologia , Mycobacterium leprae/imunologia , Adolescente , Adulto , Idoso , Antígenos de Bactérias/sangue , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Hanseníase/sangue , Modelos Lineares , Masculino , Pessoa de Meia-Idade
4.
Med Mycol ; 36(6): 413-7, 1998 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-10206752

RESUMO

Lobomycosis and paracoccidioidomycosis are two different mycoses caused by Loboa loboi and Paracoccidioides brasiliensis, respectively. To verify cross-antigenicity between them, lobomycosis sera were tested by immunoblotting, ELISA and capture-EIA against crude exo-antigen, 'cell-free antigen' and gp43 from P. brasiliensis. The majority of lobomycosis serum samples recognized crude exo-antigens and gp43 from P. brasiliensis. Gp43 was eluted from an affinity column prepared with IgG from a patient with active lobomycosis. In lower frequencies and intensities, lobomycosis sera also recognized proteins of 29 kDa, 36 kDa, 39 kDa, 52 kDa, 63 kDa, 70 kDa, 83 kDa, and 108 kDa from P. brasiliensis.


Assuntos
Antígenos de Fungos/imunologia , Entomophthorales/imunologia , Proteínas Fúngicas , Glicoproteínas/imunologia , Oligossacarídeos/imunologia , Paracoccidioides/imunologia , Zigomicose/microbiologia , Anticorpos Antifúngicos/sangue , Anticorpos Antifúngicos/imunologia , Cromatografia de Afinidade , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática , Humanos , Immunoblotting , Técnicas Imunoenzimáticas , Zigomicose/imunologia
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