RESUMO
Oscillating Low Frequency Electro-Magnetic Fields action on eye retina restoration in Rattus Norvegicus was studied in the present work. A beneficial effect of 3-Dimention Oscillating Low Frequency Electro-Magnetic Field was found for the specific values of Electro-Magnetic Field parameters. We found that eye retina damaged by radiation of the fundamental frequency harmonic of a YAG laser has recovered earlier and rehabilitated to the original 3D-state in the presence of OLFEMF, with the parameters listed below in the text. The results obtained were explained by the action of oscillating sub-macro-motions in the cells upon the metabolic processes in these cells.
Assuntos
Campos Eletromagnéticos/efeitos adversos , Lasers/efeitos adversos , Lesões Experimentais por Radiação/etiologia , Retina/lesões , Retina/efeitos da radiação , Animais , Feminino , Masculino , Lesões Experimentais por Radiação/fisiopatologia , Ratos , Regeneração/efeitos da radiação , Retina/fisiologiaRESUMO
Exchange resonance spectra of three sandwich devices containing nanolayers of Cr, Mn, Co, Ni, and Eu were recorded at 77 K. We found that these spectra are significantly different from those obtained earlier for Fe-SiO2-Au three-layer nanosandwich device. Detailed theoretical approach was developed to analyze the recorded spectra, g-factor values, and relaxation properties of the spin-polarized states in the nanolayers. We found that the g-factor values and spin-lattice relaxation rates may be adequately described by the spin-orbit scattering mechanism. Electric charge density fluctuations may also contribute to spin-lattice relaxation in nanolayers. Second-order effects in the relaxation mechanism were also briefly considered.
RESUMO
A combination of FTIR and UV spectroscopy is proposed as a novel technique for integrated real-time monitoring of metabolic activity and growth rates of cell cultures, required for systematic studies of cellular low-frequency (LF) electric and magnetic field (EMF) effects. As an example, we investigated simultaneous influence of periodic LF 3D EMFs on a culture of Saccharomyces cerevisiae (baker's yeast) cells. Amplitudes, frequencies and phases of the field components were the variable parameters. Electromagnetic fields were found to efficiently control the activity of the yeast cells, with the resulting CO(2) production rates, as monitored by FTIR spectroscopy, varying by at least one order of magnitude due to the field action. Additionally, population dynamics of the yeast cells was monitored by UV absorption of the yeast culture at λ(prob) = 320 nm, and compared to the CO(2) production rates. The detected physiologically active frequencies are all below 1 kHz, namely, 800 Hz excitation was effective in reducing the metabolic rates and arresting cell proliferation, whereas 200 Hz excitation was active in accelerating both cell proliferation and overall metabolic rates. The proposed methods produce objective, reliable and quantitative real-time results within minutes and may be used in various tasks that could benefit from a rapid feedback they provide in the form of metabolic and growth rates. Amplitude and frequency dependences of the LF EMF effects from individual field components with different polarizations were recorded and qualitatively interpreted based on a simple model, describing ion diffusion through a membrane channel.
Assuntos
Campos Eletromagnéticos , Saccharomyces cerevisiae/efeitos da radiação , Espectrofotometria Ultravioleta/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Dióxido de Carbono/metabolismo , Dióxido de Carbono/efeitos da radiação , Proliferação de Células/efeitos da radiação , Células Cultivadas , Relação Dose-Resposta à Radiação , Nitrogênio/metabolismo , Nitrogênio/efeitos da radiação , Oxigênio/metabolismo , Oxigênio/efeitos da radiação , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/crescimento & desenvolvimento , Saccharomyces cerevisiae/metabolismoRESUMO
The dynamics of photoinduced reactions between electronically excited SO(2) molecule (A (1)A(2)<--X (1)A(1)) and acetylene molecule (X (1)Sigma(g) (+)) in the SO(2)-acetylene van der Waals (vdW) complexes (clusters) was studied. The SO(2) molecule was excited by frequency-doubled radiation of a tunable dye laser, and resonance enhancement multiphoton photoionization of the produced photofragments was induced by ArF (193 nm) laser radiation or by frequency-doubled radiation of a second tunable dye laser to observe the C(2)H radical. The HOSO radical was detected by its IR emission. We found that the main photodecomposition channel of the vdW complexes (clusters) involves the SO(2) ( *)+C(2)H(2)-->HOSO+C(2)H reaction. Indeed, the analysis of the action spectra of the excitation laser radiation showed that the photofragments emerging in our experimental conditions (SO(2), 5%; C(2)H(2), 5%; and Xe; P(0)=2 atm) originate from the SO(2)...C(2)H(2) vdW complex (cluster). We analyzed the structure of this vdW complex theoretically, obtaining C(s) symmetry, with the acetylene molecule located above the OSO plane. The resonance-enhanced multiphoton photoionization action spectra of the C(2)H (A<--X) photofragmentation and the IR emission spectra of the HOSO radical allowed the authors to probe the energy distribution between the photofragments formed. The reaction that involves transition of the acetylene H atom to the SO(2) oxygen should be the primary step of the process considered, followed by nonstatistic dissociation of the vdW complex (cluster), with the C(2)H radical formed in its vibrationless state and excited both rotationally and translationally, and the HOSO radical excited vibrationally, rotationally and translationally. The proposed reaction mechanism was discussed, employing transition-state and Rice-Ramsperger-Kassel-Marcus (RRKM) approaches. The kinetics of photofragment formation was investigated, yielding characteristic radical build-up time of 0.64 micros.
RESUMO
The dynamics of the IR emission induced by excitation of the acetylene molecule at the 3(2) Ka2, A1Au<--4(1) la1, X1Sigmag+ transition was investigated. Vibrationally resolved IR emission spectra were recorded at different delay times after the laser excitation pulse. The observed IR emission was assigned to transitions between vibrational levels of the acetylene molecule in the ground state. Values of the relaxation parameters of different vibrational levels of the ground state were obtained. The Ti-->Tj transition was detected by cavity ring-down spectroscopy in the 455 nm spectral range after excitation of the acetylene molecule at the same transition. Rotationally resolved spectra of the respective transition were obtained and analyzed at different delay times after the laser excitation pulse. The dynamics of the S1-->Tx-->T1-->S0 transitions was investigated, and the relaxation parameter values were estimated for the T1 state.