RESUMO
Aerobic and methanogenic consortia were evaluated as inocula for laboratory scale denitrifying reactors, fed with a synthetic wastewater with acetate as the main electron donor. The denitrifying microflora of inocula and reactors was evaluated by specific denitrifying activity, enumeration and isolation of denitrifiers, which were screened by amplified ribosomal DNA restriction analysis. Reactor performance was monitored by COD and nitrate removal efficiencies and granule size. The aerobic sludge failed to form granules, probably due to the development of a filamentous, nitrate-reducing organism which was characterised by 16SrDNA sequencing as Bacillus cereus. The methanogenic sludge showed denitrifying activity and adapted very rapidly to denitrifying conditions in the two reactors seeded with granules of different sizes. Denitrifiers grew around the granules, increasing the specific denitrifying activity of the sludge over 10-fold. Exopolymer-forming organisms, belonging to the same species, were isolated from both reactors. Granule size increased during operation, but flotation of the aggregates, related to gas retention was observed.
Assuntos
Bacillus cereus/fisiologia , Bactérias Aeróbias/fisiologia , Reatores Biológicos , Euryarchaeota/fisiologia , Eliminação de Resíduos Líquidos/métodos , Acetatos/metabolismo , DNA Bacteriano/análise , Nitratos/metabolismo , Tamanho da Partícula , Polímeros , RNA Ribossômico 16S/análiseRESUMO
In this report, we present the characterization of a humanized monoclonal antibody specific for the human epidermal growth factor receptor (hEGFR). Direct analysis by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) of peptide mixtures and chromatographically isolated fractions allowed identification of 94.0% and 85.4% of the amino acid sequence of light and heavy chains, respectively. Microheterogeneity sources were identified in light and heavy chains and a previously unreported posttranslational modification for immunoglobulins was found. One N-glycosylation site was identified in the heavy chain with non-sialylated bianntenary fucosylated structures. This study is one of the first to assess the potential of MALDI-MS in combination with more conventional protein chemistry techniques for the characterization of monoclonal antibodies.
Assuntos
Anticorpos Monoclonais/química , Cromatografia Líquida/métodos , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Sequência de Aminoácidos , Anticorpos Monoclonais/isolamento & purificação , Humanos , Dados de Sequência Molecular , Mapeamento de Peptídeos , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Tripsina/químicaRESUMO
El presente trabajo consiste en el estudio de una planta de tratamiento de aguas residuales de frigorífico donde se procesan 650 bovinos por día. Se evaluó un sistema de tratamiento realizado en base a instalaciones existentes, sin introducir modificaciones significativas. Las instalaciones existentes permiten realizar tratamientos primarios (zarandas y flotación) y secundarios (reactor anaerobio y lagunas). En base a la evaluación primaria se concluyó que la presencia de sólidos suspendidos y grasas ocasiona problemas en los tratamientos biológicos. Para mejorar la eficiencia de los tratamientos primarios se realizaron ensayos de flotación con aire a presión obteniendo importantes mejoras(AU)
Assuntos
Águas Residuárias , Estações de Tratamento de Águas Residuárias/métodos , UruguaiRESUMO
El presente trabajo consiste en el estudio de una planta de tratamiento de aguas residuales de frigorífico donde se procesan 650 bovinos por día. Se evaluó un sistema de tratamiento realizado en base a instalaciones existentes, sin introducir modificaciones significativas. Las instalaciones existentes permiten realizar tratamientos primarios (zarandas y flotación) y secundarios (reactor anaerobio y lagunas). En base a la evaluación primaria se concluyó que la presencia de sólidos suspendidos y grasas ocasiona problemas en los tratamientos biológicos. Para mejorar la eficiencia de los tratamientos primarios se realizaron ensayos de flotación con aire a presión obteniendo importantes mejoras