RESUMO
Mephedrone (MEP) is an illicit stimulant drug that belongs to the synthetic cathinone (SC) class, which has been widely used for recreational purposes and reported in forensic analysis. The preliminary identification of MEP and other SCs in seized samples is of great interest for forensic investigation and a fast and simple screening test for these drugs would be useful for on-site and in-house analyses. In this study, we present the electrochemical detection of MEP in forensic samples using, for the first time, independent redox processes of SCs on a graphene screen-printed electrode (SPE-GP). The proposed method for MEP detection on the SPE-GP was optimized in Britton-Robinson buffer solution (0.1 mol L-1) at pH 10.0 with adsorptive stripping differential pulse voltammetry (AdSDPV). The use of the SPE-GP with AdSDPV provides a wide linear range for MEP determination (2.6 to 112 µmol L-1) with a low limit of detection (LOD) (0.3 µmol L-1). The real surface area available for adsorption on the SPE-GP was estimated to be between 3.80 and 5.70 cm2, which provided high sensitivity for the proposed method. Furthermore, good stability of MEP electrochemical responses on the SPE-GP was obtained using the same or different electrodes (N = 3), with relative standard deviation (RSD) < 5.0% for both redox processes. Interference studies for a common adulterant (caffeine) and twelve other illicit drugs (phenethylamines, amphetamines, and other SCs) were performed with a highly selective response for MEP detection. Therefore, the SPE-GP with AdSDPV is demonstrated to be a selective and sensitive screening method to detect MEP and other SCs in forensic analysis, providing a fast and simple preliminary identification of these drugs in seized samples.
Assuntos
Grafite , Metanfetamina , Catinona Sintética , Eletrodos , Técnicas Eletroquímicas/métodosRESUMO
An optical sensor (OS) was synthesized by mixing 10,12-pentacosadiinoic acid (PDA) with a triblock copolymer for use in the detection/quantification of lidocaine (LD) in seized cocaine hydrochloride (seized CH) samples. In the presence of LD, the OS presented a chromatic transition from blue to red, while no chromatic transition was observed for other typical cocaine adulterants or cocaine hydrochloride. Isothermal titration calorimetry analysis revealed specific interactions between the PDA molecules of the OS and the LD molecules, with these interactions being enthalpically favorable (-1.20 to -36.7 kJ mol-1). Therefore, the OS color change only occurred when LD was present in the sample, making the OS selective for LD. Consequently, LD was successfully detected in seized CH samples, irrespective of the type of adulteration. The OS was used for the quantification of LD in seized CH samples containing different adulterants, providing a linear range of 0.0959 to 0.225% (w/w), a precision of 7.2%, an accuracy ranging from -10 to 10%, and limits of detection and quantification of 0.0110% (w/w) and 0.0334% (w/w), respectively.