RESUMO
Squamous cell carcinoma (SCC) is a frequent malignant neoplasm of the skin that usually arises from areas of solar dermatosis. It is characterized by local invasiveness and regional lymph node metastasis, mainly in poorly differentiated tumours. Galectin-3 (Gal-3) is a lectin that is expressed in the nucleus or cytoplasm and has been identified as a prognostic tool for human neoplasms. The purpose of this study was to characterize Gal-3 expression in canine cutaneous SCCs and to investigate its relationship with tumour differentiation and cell proliferation indices. Immunohistochemical analysis of 50 SCCs for Gal-3 revealed no correlation between the localization or intensity of immunolabelling, or number of immunopositive cells, with histological grade of tumour or proliferative activity. The results suggest that Gal-3 expression is not a reliable prognostic marker for cutaneous SCC in dogs.
Assuntos
Carcinoma de Células Escamosas/veterinária , Doenças do Cão , Galectina 3/metabolismo , Animais , Biomarcadores Tumorais , Carcinoma de Células Escamosas/patologia , Proliferação de Células , Cães , Imuno-Histoquímica/veterinária , Índice Mitótico/veterinária , Gradação de Tumores/veterinária , Prognóstico , Neoplasias Cutâneas/patologia , Neoplasias Cutâneas/veterináriaRESUMO
Engorged females of Aedes albopictus, Ochlerotatus scapularis, Culex nigripalpus and Culex quinquefasciatus were collected by aspiration and sweep net during two years in Tremembé county, State of Säo Paulo, Brazil. Of the 1,092 specimens analyzed with the precipitin test, 87.6% reacted to one or more of the eight antisera tested. Of the four species for which the host determination was made, the reaction in 98.5% was to a single host. The application of the feeding index for four species of mosquitoes in Tremembe represented an attempt to measure and compare mosquito feeding patterns on these domestic hosts to evaluate the potential risk the region presents for the introduction and dissemination of arthropod-borne diseases. The results of the feeding index showed that Ae. albopictus commonly fed on humans and cattle; Oc. scapularis fed more upon cattleand dogs; Cx. nigripalpus fed on a wide range of hosts, and Cx. quinquefasciatus presented similar behavior but humans and dogs were the most common. The analysis of the feeding index agrees with the reported host feeding patterns of the four species investigated.
Assuntos
Culicidae , Comportamento Alimentar , Insetos Vetores , Animais , Brasil , Bovinos , Cidades , Transmissão de Doença Infecciosa , Cães , HumanosRESUMO
OBJECTIVE: Aedes albopictus populations can breed in several kinds of containers, and its presence has also been reported in Bromeliaceae. The purpose of this study was to evaluate the epidemiological importance of the Bromeliaceae plants as potential breeding sites of Aedes albopictus and to document the associated Culicidae entomofauna found in this micro-habitat. METHODS: Collections of Culicidae larvae were carried out fortnightly in aquatic content of bromeliads during 1998 and 1999. Collections took place in urban and periurban areas of Ilhabela island and periurban area of Ilha Comprida island, Brazil. RESULTS: A total of 26,647 Culicidae larvae were collected at both study sites, 14,575 in the urban area and 10,987 in the periurban area of Ilhabela and the remaining 1,085 in the periurban area of Ilha Comprida. There was no statistical difference between the amount of larvae collected in urban and periurban areas of Ilhabela. Regarding the Ae. albopictus, there was found a higher frequency and amount of larvae in the urban area of Ilhabela, followed by the periurban area in the same site, whereas in the periurban area of Ilha Comprida, its presence was considered accidental, since it was reported only in the first three months. CONCLUSIONS: The results show that bromeliads, largely used in landscaping, may contribute to the spread of Ae. albopictus. The presence of this species in domesticated and domiciled bromeliads allows us to suggest that the Culicidae larva is part of the regional fauna and facilitate the contact between humans and etiological agents of the natural ecosystem.
Assuntos
Aedes/fisiologia , Insetos Vetores/fisiologia , Animais , Brasil , Reservatórios de Doenças , Vetores de Doenças , Ecologia , Meio Ambiente , Larva/fisiologia , Plantas , Características de Residência , População UrbanaRESUMO
A breeding place of Aedes aegypti immature forms were found in bromeliads domesticated for decorative purposes. Implications for the control measures were considered.
Assuntos
Aedes/crescimento & desenvolvimento , Plantas/parasitologia , Animais , EcologiaAssuntos
Aedes , Solo/parasitologia , Aedes/crescimento & desenvolvimento , Animais , Brasil , Ecossistema , LarvaRESUMO
A breeding place of immature stages of Aedes albopictus in bromeliads is described. The epidemiological role of bromeliaceae as natural or artificial containers, is discussed. The ability of the mosquito to expand its habitat calls for attention especially as regards its adaptability as an invading species.
Assuntos
Aedes/crescimento & desenvolvimento , Reservatórios de Doenças , Plantas , Animais , Vetores de DoençasRESUMO
Immature stages of Anopheles bellator and of An. argyritarsis were found in artificial containers. The great number of on-returnable containers now produced, may be playing an important role in the increase in the number of available breeding places for culicid mosquitoes.
Assuntos
Anopheles/crescimento & desenvolvimento , Reservatórios de Doenças , AnimaisRESUMO
New findings of Anopheles mosquitoes in artificial containers are reported. In one, a plastic container served as a breeding place for Anopheles bellator larvae and, in another, four instar larvae of An. albitarsis s.l. were found in an abandoned toilet basin. Reflections are offered as to the selective pressure represented by the production, of an ever increasing scale, of disposable objects.
Assuntos
Anopheles/crescimento & desenvolvimento , Reservatórios de Doenças , Animais , LarvaRESUMO
INTRODUCTION: The epidemiological role of Aedes albopictus has been investigated in the State of S. Paulo by the study of its biological and ecological characteristics. The biting activity of Ae.albopictus taking stationary and moving collectors as parameters, is determined. MATERIAL AND METHOD: The study area was a small farm located in the periurban zone of Tremembé city, Vale do Paraíba, State of S. Paulo, Brazil. Aedes albopictus was caught by using two human-bait modalities. One lasted 24 hours as the collectors remained sitting in only one place and in the other the collectors visited 48 different points for five minutes each over a total period of four hours in the morning and the afternoon. Both catches were made once a month for the period of a year (1989/90). RESULTS: The 24 catches undertaken yielded 637 females of Ae.albopictus, of which 54 (8.4%) and 583 (91.6%) corresponded, respectively to fixed and moving human-bait conditions. An analysis of the data was made to discover the influence of host movement as attracting stimulus for Ae.albopictus. The biting activity took place during the day with peaks at 6:00 a.m., 1:00-2:00 p.m. and the highest between 4:00 and 5:00 p.m. The majority of the adults were collected during the summer and autumn and the moving catches were positive for Ae.albopictus throughout the year. Rainfall and rising temperature were correlated to the abundance of this species. CONCLUSION: This study has shown the complex influence of the endogenous and exogenous factors relating to the blood feeding habit of Ae.albopictus. However, it seems clear that its biting behavior depends on two distinct flights. On one, the blood feeding is obtained by the flight direct to the host, over a small supposedly short distance, and another less significant apetente flight when collectors were in a stationary position. The biting activity took place during the day and may occur all year round.
Assuntos
Aedes/fisiologia , Comportamento Alimentar , Animais , Brasil , Dengue/transmissão , Vetores de Doenças , Feminino , Estações do Ano , Fatores de TempoRESUMO
The eggs of Anopheles laneanus Correa and Cerqueira and Anopheles antunesi Galvão and Amaral are described and illustrated with scanning electron micrographs. The egg of An. laneanus is compared with those of Anopheles cruzii Dyar and Knab and Anopheles bellator Dyar and Knab, and An. antunesi is compared with Anopheles lutzii Cruz and other Nyssorhynchus species.