RESUMO
Giardia duodenalis is considered the most common protozoan infecting humans worldwide. Molecular characterization of G. duodenalis isolates has revealed the existence of eight groups (assemblages A to H) which differ in their host distribution. A cross-sectional study was conducted in 639 children from La Habana between January and December 2013. Two assemblage-specific PCRs were carried out for the molecular characterization. The overall prevalence of Giardia infection was 11.9%. DNA from 63 of 76 (82.9%) samples was successfully amplified by PCR-tpi, while 58 from 76 (76.3%) were detected by PCRE1-HF. Similar results by both PCRs were obtained in 54 from 76 samples (71%). According to these analyses, assemblage B and mixed assemblages A + B account for most of the Giardia infections in the cohort of children tested. Our current study identified assemblage B as predominant genotype in children infected with Giardia. Univariate analysis indicated that omission of washing hands before eating and keeping dogs at home were significant risk factors for a Giardia infection. In the future, novel molecular tools for a better discrimination of assemblages at the subassemblages level are needed to verify possible correlations between Giardia genotypes and symptomatology of giardiasis.
RESUMO
Se informan 30 hongos fitopatógenos que afectan a 31 plantas medicinales que provocan diferentes enfermedades en las mismas; se ofrecen sus ubicaciones taxonómicas, grado de infección en los hospederos y parte afectada; estos hongos se incluyen en 13 géneros, de ellos 3 pertenecieron a los Basidiomycetes y 27 a los hongos imperfectos. Las muestras se colectaron en los viveros de la Estaciòn Experimental de Plantas Medicinales "Dr. Juan Tomás Roig" en zonas rurales de los muncipios de San Antonio de los Baños y Güira de Melena
Assuntos
Fungos/patogenicidade , Plantas Medicinais/microbiologiaRESUMO
Se estudia la incidencia de algunos hongos patógenos que afectan diferentes especies de plantas medicinales en algunas regiones de nuestro país. Se ofrecen las sintomatologías de las enfermedades y las características morfológicas de los patógenos que las provocan