RESUMO
Abstract Objective: To evaluate the periapical healing following root canal treatment in teeth with apical periodontitis (in vivo) and the cytotoxic potential of root canal sealers in vitro. Material and Methods: Apical periodontitis was induced in 60 dogs' teeth and root canals were filled with Sealapex (40 roots), EndoREZ (40 roots), intracanal dressing (20 roots), or left untreated (20 roots). After 30 and 90 days, histopathological analyses were made. In vitro, J774.1 macrophages were stimulated with root canal sealers extracts, cytotoxicity was assessed using lactate dehydrogenase assay, and qRT-PCR was used to analyze TNF-α gene expression. Results: In vivo, smaller apical periodontitis and lower inflammatory cell infiltrate were found in teeth treated with Sealapex compared to EndoREZ. In vitro, EndoREZ was cytotoxic and induced TNF-α gene expression by macrophages differently from Sealapex. Conclusion: Sealapex allowed improved tissue repair following root canal treatment in teeth with apical periodontitis compared to EndoREZ. Synthesis of TNF-α induced by LPS was enhanced by EndoREZ, whereas Sealapex prevented pro-inflammatory gene expression (AU).
Assuntos
Animais , Cães , Periodontite Periapical , Obturação do Canal Radicular , Técnicas In Vitro , Cavidade Pulpar , Endodontia , Análise de VariânciaRESUMO
INTRODUCTION: Assessment of two radiographic images reveals two distinct, extreme situations of physiological tooth resorption, characteristic of primary teeth with or without permanent successor, due to partial anodontia. DISCUSSION: In all primary teeth, rhizolysis begins after the completion of formation, thanks to the apoptosis of their cells. When apoptosis induced by cementoblasts has denuded the root of these cells, the process of rhizolysis inevitably begins: This will be accelerated by mediators arising from the pericoronal follicle. When there is no permanent successor due to partial anodontia, rhizolysis occurs extremely slowly, and months later, without the epithelial rests of Malassez that were dead due to apoptosis, alveolodental ankylosis becomes established, and the tooth will gradually be replaced by bone, still within a physiological context. CONCLUSION: Rhizolysis and physiological tooth resorption may occur rapidly or slowly, early or late, and this depends on the presence of the permanent tooth, or its absence due to partial anodontia.
Assuntos
Reabsorção da Raiz , Reabsorção de Dente , Cemento Dentário , Dentição Permanente , Humanos , Reabsorção da Raiz/diagnóstico por imagem , Reabsorção da Raiz/etiologia , Reabsorção de Dente/diagnóstico por imagem , Dente DecíduoRESUMO
Knife-edge or blunt root resorptions characterize ameloblastomas and are pathognomonic for this tumor, because they differentiate ameloblastomas from simple bone cysts, odontogenic keratocysts and nasopalatine duct cysts, which do not lead to resorption of involved teeth. Despite the very high frequency and importance of these characteristics for a differential diagnosis, a microscopic examination should also be conducted before defining the diagnosis and the treatment plan for these cases. This paper describes a six-step hypothesis to explain the mechanism by which ameloblastomas promote the characteristic root resorptions found in association with these benign epithelial tumors, which have a fibrous capsule formed by islands and epithelial cords that mimic the dental lamina, invade neighboring tissues and release mediators (IL-1, EGF) of tooth and root resorption. This hypothesis may be one more explanation for the tooth resorptions sometimes found in orthodontic records, and may help differentiate the root resorptions that are specific to the orthodontic practice.
Assuntos
Ameloblastoma , Cistos Odontogênicos , Reabsorção da Raiz , HumanosRESUMO
ABSTRACT Knife-edge or blunt root resorptions characterize ameloblastomas and are pathognomonic for this tumor, because they differentiate ameloblastomas from simple bone cysts, odontogenic keratocysts and nasopalatine duct cysts, which do not lead to resorption of involved teeth. Despite the very high frequency and importance of these characteristics for a differential diagnosis, a microscopic examination should also be conducted before defining the diagnosis and the treatment plan for these cases. This paper describes a six-step hypothesis to explain the mechanism by which ameloblastomas promote the characteristic root resorptions found in association with these benign epithelial tumors, which have a fibrous capsule formed by islands and epithelial cords that mimic the dental lamina, invade neighboring tissues and release mediators (IL-1, EGF) of tooth and root resorption. This hypothesis may be one more explanation for the tooth resorptions sometimes found in orthodontic records, and may help differentiate the root resorptions that are specific to the orthodontic practice.
RESUMO As reabsorções radiculares "em plano" ou "em corte" são características do ameloblastoma e foram consideradas patognomônicas dessa lesão, diferenciando-o do cisto ósseo simples, queratocisto odontogênico e do cisto nasopalatino - nos quais elas estão ausentes, nos dentes envolvidos. Apesar dessa frequência elevadíssima e importante no diagnóstico diferencial, não se pode dispensar o exame microscópico, para um diagnóstico definitivo, no planejamento terapêutico do caso. Elaborou-se uma hipótese com seis passos para explicar o mecanismo pelo qual o ameloblastoma promove essa típica reabsorção radicular peculiar da lesão: ser uma neoplasia epitelial benigna sem cápsula fibrosa, formada por ilhotas e cordões epiteliais que imitam a lâmina dentária e invadem os tecidos vizinhos, liberando mediadores (IL-1, EGF) da reabsorção óssea e dentária. Essa hipótese ajuda a explicar mais uma das causas de reabsorções dentárias que podem estar presentes nas análises das documentações ortodônticas, ajudando a diferenciar as reabsorções radiculares próprias da prática ortodôntica.