RESUMO

Lateral (L) and medial (M) midbrain astrocytes differ in their ability to support neuritic growth (L, permissive; M, non-permissive) with properties of M glia depending on heparan sulfate (HS). Here we show by atomic force microscopy that the surfaces of formaldehyde-fixed astrocytes differ by conspicuous 250 nm protrusions in L and by a HS-dependent fibrillar network in M glia, thus, demonstrating correlations between cell surface morphology and functional properties.

Assuntos

Astrócitos/citologia , Astrócitos/fisiologia , Microscopia de Força Atômica , Neuritos/fisiologia , Animais , Astrócitos/efeitos dos fármacos , Membrana Celular/ultraestrutura , Fixadores , Formaldeído , Heparitina Sulfato/farmacologia , Mesencéfalo/citologia , Camundongos/embriologia , Rede Nervosa/efeitos dos fármacos , Rede Nervosa/fisiologia , Neurofibrilas/fisiologia