RESUMO
Malignant fibrous histiocytoma (MFH) is a type of highly malignant soft tissue sarcoma with a predilection for the extremities of adults. We report a patient with MFH in the infraspinatus muscle for which wide resection including total resection of the infraspinatus muscle was performed, followed by transfer of the latissimus dorsi muscle for shoulder reconstruction in a one-stage operation with good postoperative function.
Assuntos
Histiocitoma Fibroso Maligno/cirurgia , Músculo Esquelético/patologia , Músculo Esquelético/cirurgia , Procedimentos de Cirurgia Plástica/métodos , Ombro/cirurgia , Neoplasias de Tecidos Moles/cirurgia , Retalhos Cirúrgicos , Adulto , Biópsia , Meios de Contraste , Feminino , Gadolínio DTPA , Histiocitoma Fibroso Maligno/diagnóstico , Humanos , Imageamento por Ressonância Magnética , Neoplasias de Tecidos Moles/diagnóstico , Tomografia Computadorizada por Raios XRESUMO
Malignant fibrous histiocytoma (MFH) is a type of highly malignant soft tissue sarcoma with a predilection for the extremities of adults. We report a patient with MFH in the infraspinatus muscle for which wide resection including total resection of the infraspinatus muscle was performed, followed by transfer of the latissimus dorsi muscle for shoulder reconstruction in a onestage operation with good postoperative function.
El histiocitoma fibroso maligno (HFM) es un tipo de sarcoma del tejido suave, altamente maligno, con predilección por las extremidades de los adultos. Reportamos el caso de un paciente con HFM en el músculo infraespinoso, al cual se le hizo una amplia resección que incluyó la resección total del músculo infraespinoso, seguida de una transferencia del músculo latissimus dorsi para la reconstrucción del hombro, en una operación de una sola etapa con buena función postoperatoria.
Assuntos
Adulto , Feminino , Humanos , Histiocitoma Fibroso Maligno/cirurgia , Músculo Esquelético/patologia , Músculo Esquelético/cirurgia , Procedimentos de Cirurgia Plástica/métodos , Ombro/cirurgia , Neoplasias de Tecidos Moles/cirurgia , Retalhos Cirúrgicos , Biópsia , Meios de Contraste , Gadolínio DTPA , Histiocitoma Fibroso Maligno/diagnóstico , Imageamento por Ressonância Magnética , Neoplasias de Tecidos Moles/diagnóstico , Tomografia Computadorizada por Raios XRESUMO
The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low toxicity. Yeast DNA microarray analysis revealed induction of genes whose products were localized to the membranes, and of genes that are involved in or contribute to energy production. Furthermore, we found that nitrogen gas significantly affected the transport system in the cells. Interestingly, nitrogen gas also resulted in induction of cold-shock responsive genes. These results suggest the possibility of applying yeast DNA microarray to gas bioassays up to 40 MPa. We therefore think that "bioassays" are ideal for use in environmental control and protection studies.
Assuntos
Regulação Fúngica da Expressão Gênica , Pressão Hidrostática , Nitrogênio , RNA Fúngico/análise , Saccharomyces cerevisiae/genética , Estudos de Viabilidade , Perfilação da Expressão Gênica , Regulação Fúngica da Expressão Gênica/genética , Análise de Sequência com Séries de Oligonucleotídeos , RNA Mensageiro/análise , Saccharomyces cerevisiae/citologiaRESUMO
The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low toxicity. Yeast DNA microarray analysis revealed induction of genes whose products were localized to the membranes, and of genes that are involved in or contribute to energy production. Furthermore, we found that nitrogen gas significantly affected the transport system in the cells. Interestingly, nitrogen gas also resulted in induction of cold-shock responsive genes. These results suggest the possibility of applying yeast DNA microarray to gas bioassays up to 40 MPa. We therefore think that "bioassays" are ideal for use in environmental control and protection studies.
Assuntos
Regulação Fúngica da Expressão Gênica , Pressão Hidrostática , Nitrogênio , RNA Fúngico/análise , Saccharomyces cerevisiae/genética , Estudos de Viabilidade , Perfilação da Expressão Gênica , Regulação Fúngica da Expressão Gênica/genética , Análise de Sequência com Séries de Oligonucleotídeos , RNA Mensageiro/análise , Saccharomyces cerevisiae/citologiaRESUMO
The merozoite surface protein-1 (MSP-1) is a major vaccine candidate for the asexual blood stage of malaria. We examined both the extent of sequence diversity in block 17, the 3' end of Msp-1 gene coding for a 19-kDa polypeptide (MSP-1(19)) putatively involved in red blood cell binding, and the patterns of linkage disequilibrium between polymorphic sites throughout the Msp-1 locus. The parasite population sample consisted of Plasmodium falciparum isolates collected between 1985 and 1998 in Rondjnia, an area of hypoendemic malaria transmission in the southwestern Brazilian Amazon. Results were summarized as follows. (1) Seven block-17 sequence variants or haplotypes were found among 130 isolates, including two new haplotypes (novel combinations of previously reported amino acid replacements), here named Brazil-1 (E-TSR-F) and Brazil-2 (Q-TSR-F). (2) As previously shown for other Msp-1 polymorphisms, frequencies of block-17 haplotypes displayed significant temporal variation. (3) Extensive linkage disequilibrium was demonstrated between neighboring dimorphic sites within block 17, as well as between polymorphisms at the 5' and 3' ends of Msp-1 (map distance range: 3.83-4.99 kb). (4) The overall patterns of linkage disequilibrium within Msp-1 remained stable over a period of nearly one decade, and examples of possible 'epidemic' expansion of parasites carrying particular Msp-1 alleles were found in the 1980s and 1990s. These results are discussed in relation to the population biology of P. falciparum and the development of malaria vaccines based on MSP-1.
Assuntos
Variação Genética , Desequilíbrio de Ligação , Proteína 1 de Superfície de Merozoito/genética , Plasmodium falciparum/genética , Alelos , Sequência de Aminoácidos , Animais , Brasil/epidemiologia , Haplótipos , Humanos , Vacinas Antimaláricas , Dados de Sequência Molecular , Plasmodium falciparum/classificação , Reação em Cadeia da Polimerase/métodos , Fatores de TempoRESUMO
To evaluate the effectiveness of gammaglobulin in decreasing the incidence of coronary artery lesions in Kawasaki disease, a randomized controlled study in 136 patients was conducted using high doses of gammaglobulin 400 mg/kg/d for 3 days plus aspirin 30 mg/kg/d (gammaglobulin group) and aspirin alone at the same dosage (aspirin group). The total febrile period and the duration of fever after treatment were significantly shorter in the gammaglobulin group than in the aspirin group (P less than 0.001). The incidence of coronary artery lesions and of coronary artery aneurysms was significantly lower in the gammaglobulin group than in the aspirin group up to 30 days after the onset of Kawasaki disease (P less than 0.01 and P less than 0.05, respectively). In 16 of 69 patients given gammaglobulin, fever persisted for longer than 3 days, and there was a higher incidence of coronary artery lesions among them. The effectiveness of high doses of gammaglobulin in preventing coronary artery lesions has been demonstrated, but the indications and the optimal dose of gammaglobulin remain to be determined.