RESUMO
OBJECTIVE: To investigate the prevalence and seropositivity of SARS-CoV-2 in companion and exotic animals in a veterinary healthcare system. SAMPLE: A total of 341 animals were sampled by a combination of oral and nasal swabs. Serum from whole blood was collected from a subset of animals (86 canines, 25 felines, and 6 exotic animals). METHODS: After informed owner consent, convenience samples from client-owned animals and the pets of students and staff members associated with Colorado State University's Veterinary Health System were collected between May 2021 and September 2022. Study samples were collected by trained veterinarians, Veterinary Health System staff, and veterinary students. RESULTS: SARS-CoV-2 RNA was detected by reverse transcription PCR in 1.6% (95% CI, 0.5% to 4.6%) of domestic canines and 1.1% (95% CI, 0.2% to 6.1%) of domestic felines. No RNA was detected in any of the exotic animal species tested (n = 66). Plaque reduction neutralization tests indicated that 12.8% (95% CI, 7.3% to 21.5%) of canines and 12.0% (95% CI, 4.2% to 30.0%) of felines had neutralizing antibodies against SARS-CoV-2. CLINICAL RELEVANCE: This study provides insight regarding SARS-CoV-2 spillover in domestic companion and exotic animals and contributes to our understanding of transmission risk in the veterinary setting.
Assuntos
COVID-19 , Doenças do Gato , Doenças do Cão , Humanos , Animais , Gatos , Cães , COVID-19/epidemiologia , COVID-19/veterinária , RNA Viral , SARS-CoV-2 , Colorado/epidemiologia , Pessoal de SaúdeRESUMO
This article provides information to help US-based practitioners develop differential diagnoses for, and recognize foreign animal diseases associated with, dermatologic lesions in small ruminants. Sheep and goat pox are currently considered foreign animal diseases (in the United States) and may cause lesions similar to other endemic diseases of small ruminants including orf, ulcerative dermatosis, bluetongue, and dermatophilosis. Any cases involving unusual dermatologic lesions associated with high morbidity and/or mortality warrant reporting to governmental authorities including USDA APHIS or state regulatory veterinarians for herd or flock investigations. Vigilance on the part of livestock veterinarians and small ruminant producers is of paramount importance in preventing the entry and spread of economically devastating foreign animal diseases.
Assuntos
Doenças dos Animais , Doenças das Cabras , Infecções por Poxviridae , Doenças dos Ovinos , Animais , Ovinos , Estados Unidos , Cabras , Doenças das Cabras/epidemiologia , Doenças Endêmicas , Ruminantes , Infecções por Poxviridae/veterináriaRESUMO
Detection of Leptospira interrogans is difficult as a result of intermittent leptospiruria and brief leptospiremia. Hence, diagnosis relies heavily on serologic testing, the reference method of which is the microscopic agglutination test (MAT). In horses, clinical leptospirosis has been associated with abortion, recurrent uveitis, and sporadic cases of hepatic and renal disease. Little information exists on the seroprevalence of antibodies to L. interrogans in equids in the United States; past nationwide studies suggest that the seroprevalence in some areas is as high as 77% (reciprocal titer ≥ 100). We tested sera from 124 apparently healthy horses previously submitted for equine infectious anemia (EIA) serology using MAT for 6 serovars-Bratislava, Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, and Pomona. When using a reciprocal MAT titer cutoff of ≥ 100, 102 of 124 (82%) of the samples were positive for at least one serovar. Seropositivity was significantly associated with increasing age. Query of specimens from clinical cases submitted to the Colorado State University Veterinary Diagnostic Laboratory for MAT since 2010 indicated significantly greater seroprevalence (p = 0.015) of pathogenic serovar Pomona in clinical cases compared to sera submitted from healthy equids for routine EIA testing. Information from our diagnostic laboratory submission forms also suggests a correlation between uveitis or other ophthalmic problems and serovar Pomona.