RESUMO
OBJECTIVE: To identify technical problems in exhumations performed for DNA detection in bones and to propose solutions through a protocol. METHODS: A prospective and qualitative study of exhumations was carried out according to the methods proposed in the medical legal literature. From 1995 to 1998, were performed 10 exhumations to collect human remains for DNA extraction. Of them, seven cases were of civil interest and three of criminal. Alternatives were sought to overcome technical difficulties found during the execution of these procedures. RESULTS: For all cases, there was scarcity of useful information to identify the human remains. In half of them, identification was based on the individual's morphological characteristics, given by their relatives. Individual morphological characteristics contributed to identification in 50% of cases. In three cases, it was possible to determine only the sex, and in one of them, only the age. Lack of infrastructure and police security in the cemeteries impaired the examination. CONCLUSIONS: To assure the reliability of the DNA molecular examination, it is necessary to identify the individual to whom the exhumed mortal remains belonged. To an efficacious investigation, it is paramount to have a working protocol that will cover, among other issues, those concerning identification, infrastructure and staff safety at the site of examination.
Assuntos
Osso e Ossos/química , DNA/análise , Antropologia Forense/métodos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Medicina Legal/métodos , Humanos , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Estudos ProspectivosRESUMO
Homicides in the city of Ribeirão Preto, state of São Paulo, Brazil, were analyzed with regard to methods (firearms or others) and gender, in a retrospective study from 1993 to 1997. There was a progressive increase in the number of homicides, mainly after 1995, with an increment of 86.6% in 1997 in comparison to 1993. The incidence of homicides was higher among males, with firearms being the most common method in this group since 1993. In addition, a 10% increase in this method was observed since 1995, in association with a proportional reduction in the use of other methods. The incidence of homicides among females remained stable until 1996 and increased by 51.3% in 1997. Simultaneously, firearms have become the most common method among female victims (71.4%), pattern similar to that observed among males. Attention is drawn to the social and economic conditions and their relationships with drug traffic in the city.
Assuntos
Homicídio/estatística & dados numéricos , Violência/estatística & dados numéricos , Ferimentos por Arma de Fogo/mortalidade , Adolescente , Adulto , Brasil/epidemiologia , Feminino , Homicídio/tendências , Humanos , Incidência , Masculino , Distribuição por Sexo , População Urbana , Violência/tendênciasRESUMO
Machado-Joseph disease (MJD) and Holguin ataxia (SCA2) are autosomal dominant multisystem degenerations with spinocerebellar involvement that are predominant among people of Portuguese-Azorean and of Cuban descent, respectively. Their clinical distinction may at times be difficult to make in individual patients, due to significant phenotypic overlapping (similar overall age-of-onset and duration of cerebellar ataxia, eye movement, and, often, other common problems. The recent mapping of SCA2 to chromosome 12q provided another candidate region for linkage studies of MJD. Original data on 10 families with Holguin ataxia show that the locus for phenylalanine hydroxylase (PAH) on chromosome 12q is linked to SCA2 at 4 cM and is thus far its closest marker. The exclusion of linkage 15 cM on each side of PAH in 16 families with MJD shows that these two forms of dominant ataxia are genetically distinct and at different chromosomal locations (nonallelic).