RESUMO

SARS-CoV-2 is a matter of concern. Here, biosensors were prepared using iron magnetic nanoparticles containing antibodies against the receptor binding domain (RBD) of the spike protein. Antibodies were adsorbed to nanoparticles in three configurations, including direct adsorption without functionalization (DANPs). Nanoparticles were added to a glassy carbon electrode and connected to an electrochemical cell. Electrochemical impedance spectroscopy and ELISA experiments indicated that antibodies were desorbed from the DANPs upon the addition of the RBD. DANPs-based biosensors produced linear curves with decreasing charge transfer resistance due to the removal of antibodies. Thus, a detection method can be based on antibody desorption.

Assuntos

RESUMO

This work shows the efficiency of wash waters from lipopeptide production as a remediation strategy to treat urban water samples contaminated with p-cresol. The harvesting step in surfactin production involved a centrifugation step, generating a major soluble fraction and a fraction that is adsorbed to the biomass. The adsorbed fraction was recovered by washing steps. These wash waters containing lipopeptides (mostly surfactins), were successfully used to adsorb and solubilize p-cresol. The method of decontamination applied to an artificially contaminated natural water was monitored using a biosensor based on laccase/magnetic nanoparticles. Given the amount of surfactin within the wash water, the removal of p-cresol from artificially contaminated water was approximately 46.0%. This result confirms the successful and sustainable application of surfactin-rich wash waters to remove p-cresol from artificially contaminated natural water. The adsorption mechanism is potentially based on a multi-layer adsorption process, considering Langmuir and Freundlich adsorption isotherms.

Assuntos

RESUMO

Lipopeptides are important secondary metabolites produced by microbes. They find applications in environmental decontamination and in the chemical, pharmaceutical and food industries. However, their production is expensive. In the present work we propose three strategies to lower the production costs of surfactin. First, the coproduction of surfactin and arginase in a single growth. Second, extract the fraction of surfactin that adsorbs to the biomass and is removed from the growth medium through centrifugation. Third, use microbial biomass for the remediation of organic and inorganic contaminants. The coproduction of surfactin and arginase was evaluated by factorial design experiments using the LB medium supplemented with arginine. The best conditions for surfactin production were 22 h of growth at 37 °C using LB supplemented with arginine 7.3 g/L. Almost similar conditions were found to produce highest levels of arginase, 24 h and 6.45 g/L arginine. Decontamination of phenol and copper from artificial samples was attained by treatment with residues from lipopeptide production. Thus, cell suspensions and wash-waters used to extract surfactin from the biomass. Cell suspensions were used to successfully remove hydroquinone. Cell suspensions and wash-waters containing surfactin were successfully used to recover copper from solution. Specific monitoring methods were used for phenol and metal solutions, respectively a biosensor based on tyrosinase and either atomic absorption flame ionization spectrometry or absorbance coupled to the Arduino™ platform. Therefore, we report three alternative strategies to lower the production costs in lipopeptide production, which include the effective recovery of copper and phenol from contaminated waters using residues from surfactin production. Sustainable and profitable production of surfactin can be achieved by a coproduction strategy of lipopeptides and enzymes. Lipopeptides are collected in the supernatant and enzymes in the biomass. In addition, lipopeptides that coprecipitate with biomass can be recovered by washing. Lipopeptide wash-waters find applications in remediation and cells can also be used for environmental decontamination.

Assuntos

RESUMO

The cardiac troponin T (cTnT) is specific biomarker important for trials of acute myocardial infarctions (AMI). In this paper, a SPR sensor in real time to detect the biomarker was developed on a commercially available surface plasmon resonance AUTOLAB SPIRIT. The cTnT receptor molecule was covalently immobilized on a gold substrate via a self-assembled monolayer (SAM) of thiols by using cysteamine-coupling chemistry. This biosensor presented a linear response range for cTnT between 0.05 and 4.5 ng/mL (r=0.997, p<<0.01) with a good reproducibility (CV=4.4%). The effect of the cysteamine (CYS) concentrations on the SAM coated gold sensor was studied as a function of the amount of the immobilized cTnT monoclonal antibodies. Analysis using serum samples undiluted was carried out at room temperature showing a well agreement with the ECLIA methods and the sensor surface could be regenerated by using a solution of 1% (w/v) sodium dodecyl sulphate (SDS) without losing the sensor immunoreactivity. These studies open new perspectives of using SAM to develop regenerable immunosensor with a good reproducibility allowing its use in the clinical applications.

Assuntos

RESUMO

The performance of a new graphite-castor oil polyurethane composite electrode in the determination of hydroquinone in photographic developers in a differential pulse voltammetric (DPV) procedure is described. The 60% (graphite, w/w) composite electrode presented good stability, repeatability and accurate response. Limit of detection of 934 nmol L(-1) was observed for hydroquinone within a linear dynamic range from 66 to 530 nmol L(-1). The determination of hydroquinone in a photographic developer sample showed agreement with the label values in a 95% confidence level with maximum 2% relative error and recoveries between 100.1 and 100.4%. No need of sample preparation was required since the p-methylaminophenolsulfate (metol) present in the developer formulation did not interfered in the measurements.

RESUMO

A simple strategy for cysteine determination using flow-injection analysis with electrochemical detection is described. The approach is based on the chemisorptions reactions of the sulfur moiety of cysteine upon polycrystalline gold electrodes and its subsequent reductive desorption. The electrochemical measurements were accomplished by the application of differential pulse voltammetry (DPV) for the operational optimization and pulsed electrochemical detection (PED) in combination with flow-injection analysis for the electrochemical detection as time function. The electroactive species could be adsorbed in a potential level (0.1 V versus SCE), at other (-0.6 V versus SCE) occurs their reductive desorption from the electrode, while the analytical current is recorded simultaneously, and a third potential step is applied to the complete regeneration of the gold electrode surface (-1.3 V versus SCE). The linear response range was observed between 1.0 x 10(-6) and 6.0 x 10(-6) mol L(-1) with a good reproducibility (R.S.D.<3.2%) and sensitivity (1.1 microA/microM). The repeatability (a series of 27 continuous FIA peaks of 5. 0 micromol L(-1) of cysteine) was 3.8 % and the limit of detection was 5.0 x 10(-7) mol L(-1). The sample throughput was 23 samples per hour with a very high stability in its voltammetric response. The developed methodology was successfully used for the determination of cysteine in commercial supplementary food sample.