RESUMO
BACKGROUND: M2 macrophages are often detected in oral squamous cell carcinoma (OSCC), which, influenced by hypoxic conditions, appear to have high angiogenesis-inducing capacity. However, the effects of immunosenescence on tumor-associated macrophages (TAMs) and angiogenesis in OSCC are unknown. METHODS: Fifty-seven OSCCs were divided into 3 groups (I: <40 years [n = 17]; II: 40-65 years [n = 20]; III: >65 years [n = 20]). Immunohistochemistry for CD68 and CD163 (TAMs), and CD34 and D2-40 for microvessel density (MVD), microvessel area (MVA), and total vascular area (TVA) were performed. RESULTS: All groups showed similar clinicopathological and immunohistochemical findings. Similar CD68 and CD163 expression, confirmed a M2 phenotype. MVD, MVA, and TVA were similar, however, with significant predominance of blood vessels. No significant correlation between macrophage and angiogenic markers was observed. CONCLUSIONS: A similar TAM and angiogenesis profile suggests the participation of other mechanisms, instead immunosenescence, in young and elderly OSCC patients.
Assuntos
Carcinoma de Células Escamosas/imunologia , Carcinoma de Células Escamosas/patologia , Macrófagos/metabolismo , Neoplasias Bucais/imunologia , Neoplasias Bucais/patologia , Neovascularização Patológica/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos CD/metabolismo , Antígenos de Diferenciação Mielomonocítica/metabolismo , Carcinoma de Células Escamosas/metabolismo , Feminino , Humanos , Imuno-Histoquímica , Imunossenescência , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/metabolismo , Receptores de Superfície Celular/metabolismo , Microambiente TumoralRESUMO
Oral squamous papilloma (OSP) is a benign proliferation of the stratified squamous epithelium, which results in a papillary or verrucous exophytic mass. Twelve patients suspected to have oral papilloma underwent excisional biopsy for histopathologic and immunohistochemical analysis. The majority of the patients (75%) were females, and the most prevalent site was the tongue, followed by the palate. The round and whitish form was present in 58.4% of the cases. The lesions were softened/flaccid in 66.7% of cases and a pedunculated attachment was seen in 75% of the lesions. The histopathologic examination revealed hyperparakeratosis, occasional basal hyperplasia, and koilocyte-like cells in 100% of the specimens. Immunohistochemical assays utilizing BP53-12 and Pab240 antibodies for p53 protein showed negative or weak immunostaining (91.6%) for both immunomarkers in all the epithelial layers examined. The findings suggest the benign nature of the lesions and small possibility of becoming malignant.