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O Sarcoma Anaplásico Renal DICER1 é um tumor raro, com menos de 30 casos relatados desde 2007, que ocorre predominantemente no sexo feminino. É caracterizado macroscopicamente por uma grande massa renal heterogênea com áreas sólidas e císticas e histologicamente, pela presença de cistos, na maioria dos casos, e áreas sólidas compostas por células fusiformes indiferenciadas com marcada alteração anaplásica e áreas proeminentes de benignidade ou malignidade de diferenciação condróide ou cartilaginosa. Nosso caso ocorreu em um paciente do sexo masculino de 08 anos de idade que apresentava infecção do trato urinário (ITU) de repetição há dois anos, associado à dor lombar, com Tomografia Computadorizada (TC) evidenciando grande massa renal à direita. Foi operado de urgência, devido piora do quadro clínico, e suspeita de rotura intratumoral. Após nefrectomia à direita, o exame histopatológico mostrou Sarcoma anaplásico renal associado à nefroma cístico residual.

DICER1 Renal Anaplastic Sarcoma is a rare tumor, with less than 30 cases reported since 2007, which occurs predominantly in females. It is characterized macroscopically by a large heterogeneous renal mass with solid and cystic areas and histologically, by the presence of cysts, in most cases, and solid areas composed of undifferentiated spindle cells with marked anaplastic alteration and prominent areas of benignity or malignancy of chondroid differentiation or cartilaginous. Our case occurred in an 8- year-old male patient who had recurrent UTI for two years, associated with low back pain, with CT showing a big renal mass on the right. He underwent urgent surgery due to worsening of the clinical condition and suspicion of intra-tumoral rupture. Histopathological examination showed renal anaplastic sarcoma associated with residual cystic nephroma.

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Antibiotic-resistant bacteria are a growing concern for human and animal health. The objective of this study was to determine the antimicrobial resistance and extended-spectrum beta-lactamase genes in Enterobacterales, Pseudomonas spp. and Acinetobacter spp. isolates from the uterus of healthy mares. For this purpose, 21 mares were swabbed for samples, which were later seeded on blood agar and MacConkey agar. The isolates were identified using MALDI-TOF and the antimicrobial susceptibility test was performed using the Kirby-Bauer technique. To characterize the resistance genes, a polymerase chain reaction (PCR) scheme was performed. Of the isolates identified as Gram-negative, 68.8% were Enterobacterales, represented by E. coli, Enterobacter cloacae, Citrobacter spp., and Klebsiella pneumoniae; 28.1% belonged to the genus Acinetobacter spp.; and 3.1% to Pseudomonas aeruginosa. A 9.3% of the isolates were multidrug-resistant (MDR), presenting resistance to antibiotics from three different classes, while 18.8% presented resistance to two or more classes of different antibiotics. The diversity of three genes that code for ESBL (blaTEM, blaCTX-M and blaSHV) was detected in 12.5% of the strains. The most frequent was blaSHV, while blaTEM and blaCTX-M were present in Citrobacter spp. and Klebsiella pneumoniae. These results are an alarm call for veterinarians and their environment and suggest taking measures to prevent the spread of these microorganisms.

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Introdução: A procura pela estética do sorriso é crescente na Odontologia, pois, muitas vezes um sorriso alinhado e periodonto saudável não são o bastante. A cerâmica odontológica apresenta-se como uma ótima possibilidade restauradora, visto o avanço das técnicas de adesão entre o substrato dentário e as cerâmicas, torna-se mais previsível o resultado com este tipo de reabilitação. Objetivo: O presente artigo tem como objetivo descrever um caso clínico com envolvimento de diversos níveis de desgaste dentário para reabilitação estética com cerâmica de dissilicato de lítio. Relato de caso: Paciente 36 anos, gênero feminino, apresentava insatisfação com seu sorriso devido a forma e a alteração de cor dos seus dentes superiores anteriores e pré-molares. O plano de tratamento consistiu em realizar a troca da coroa metalocerâmica por coroa total cerâmica da unidade 14, coroa total cerâmica nas unidades 11, 21 e 22, facetas cerâmicas nas unidades 15, 13, 12, 23, 24 e 25, osteotomia e gengivoplastia das regiões de 2º prémolar superior esquerdo ao 2º pré-molar superior direito, clareamento dental caseiro da arcada inferior e placa oclusal superior de proteção. O material de escolha para a resolução do caso clínico foi a cerâmica vítrea reforçada à base de dissilicato de lítio, pois, possui um índice de refração de luz semelhante à estrutura dentária, sem interferência significativa da translucidez, além de ser passível de cimentação adesiva por se tratar de uma cerâmica ácido sensível e o tamanho do cristal e a sua disposição favorecem o aumento das propriedades mecânicas da restauração. Conclusão: O plano de tratamento realizado permitiu o restabelecimento da função e estética, com reprodução das características dos dentes naturais e promoção da jovialidade e da harmonia no sorriso(AU)

Introduction:The demand for smile aesthetics is growing in Dentistry, because, often a smile aligned and healthy periodontium are not enough. Dental ceramics, presented as an excellent restorative possibility, since the advancement of adhesion techniques between the dental substrate and the ceramics, becomes more predictable the result with this type of rehabilitation. Objective: This article aims to describe a clinical case involving several levels of dental wear for aesthetic rehabilitation with ceramics. Case Report: A 36-year-old female patient presented dissatisfaction with her smile due to the shape and color alteration of her anterior and premolar upper teeth. The treatment plan consisted of exchanging the metal-ceramic crown for the total ceramic crown of unit 14, total ceramic crown in units 11, 21 and 22, ceramic veneers in units 15, 13, 12, 23, 24 and 25, osteotomy and gingivoplasty of the regions from 2nd left upper premolar to 2nd right upper premolar, home tooth whitening of the lower arch and upper occlusal plate of protection. The material of choice for the resolution of the clinical case was the glass ceramic reinforced on the basis of lithium disilicate because it has a refractive index of light similar to the dental structure, without significant interference of translucency, besides being liable to adhesive cementation because it is a sensitive acid ceramic and the size of the crystal and its disposition favor the increase of the mechanical properties of the restoration. Conclusion: The treatment plan performed allowed the restoration of function and aesthetics, with reproduction of the characteristics of natural teeth and promotion of joviality and harmony in the smile(AU)

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Intracranial hematomas (ICH) are a frequent condition in neurosurgical and neurological practices, with several mechanisms of primary and secondary injury. Experimental research has been fundamental for the understanding of the pathophysiology implicated with ICH and the development of therapeutic interventions. To date, a variety of different animal approaches have been described that consider, for example, the ICH evolutive phase, molecular implications and hemodynamic changes. Therefore, choosing a test protocol should consider the scope of each particular study. The present review summarized investigational protocols in experimental research on the subject of ICH. With this subject, injection of autologous blood or bacterial collagenase, inflation of intracranial balloon and avulsion of cerebral vessels were the models identified. Rodents (mice) and swine were the most frequent species used. These different models allowed improvements on the understanding of intracranial hypertension establishment, neuroinflammation, immunology, brain hemodynamics and served to the development of therapeutic strategies.

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Schinus molle is a plant traditionally used in Mexico to treat gastric disorders. However, no scientific evidence has been reported on its gastroprotective effect. The aim of the current contribution was to conduct a bioassay-guided study on S. molle to evaluate its gastroprotective activity in a model of Wistar rats given ethanol orally to induce gastric lesions. The hexane and dichloromethane extracts from the tested plant showed over 99% gastroprotection at a dose of 100 mg/kg. From the hexane extract, two of the three fractions (F1 and F2) afforded over 99% gastroprotection. The F1 fraction was subjected to column chromatography, which revealed a white solid. Based on the ESI-MS analysis, the two main compounds in this solid were identified. The predominant compound was probably a triterpene. This mixture of compounds furnished about 67% gastroprotection at a dose of 100 mg/kg. Pretreatment with L-NAME, indomethacin, and NEM was carried out to explore the possible involvement of nitric oxide, prostaglandins, and/or sulfhydryl groups, respectively, in the gastroprotective activity of the white solid. We found evidence for the participation of all three factors. No antisecretory activity was detected (tested by pylorus ligation). In conclusion, evidence is herein provided for the first time of the gastroprotective effect of S. molle.

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Introdução: As cerâmicas odontológicas são amplamente utilizadas na Odontologia, desfrutando de grande popularidade devido a sua capacidade de mimetizar as características ópticas do esmalte e da dentina. O constante desenvolvimento de pesquisas tem desencadeado o lançamento de novos sistemas cerâmicos com propriedades aprimoradas e melhores resultados clínicos em longo prazo. Objetivo: O relato de caso aqui apresentado descreve uma reabilitação da zona estética do sorriso com laminados cerâmicos por meio de uma abordagem minimamente invasiva. Relato de caso: Paciente do gênero feminino, compareceu à clínica odontológica relatando insatisfação com a aparência do seu sorriso. Durante avaliação foram observadas, na região anterossuperior, restaurações extensas em resina composta deficientes nos incisivos, com proporção, volume, forma e texturização insatisfatórias, que em conjunto comprometia a zona estética do sorriso. Diante disso, após fotografias extra e intraorais, o tratamento proposto foi a reabilitação com laminados cerâmicos nas unidades 1.2 ao 2.2. Assim, foi realizado o enceramento e o mock-up, os quais serviram de orientação para os preparos dentários, a seguir, foram moldados e as restaurações confeccionadas no sistema IPS e.max. Após prova de cor e adaptação, os preparos e a superfície interna dos laminados cerâmicos foram tratados e cimentados com cimento resino fotopolimerizável. Conclusão: Instituindo uma abordagem conservadora, o plano de tratamento realizado através da reabilitação com laminados cerâmicos nas unidades dentárias anterossuperiores, permitiu o restabelecimento da função e estética, com reprodução das características dos dentes naturais e promoção do equilíbrio, da jovialidade e da harmonia no sorriso(AU)

Introduction: Dental ceramics are widely used in dentistry, enjoying great popularity due to the ability to mimic both enamel and dentin optical characteristics. The constant research development has triggered the launch of new ceramic systems with improved properties and better long-term clinical results. Objective: The case report presented here describes the zone rehabilitation of the aesthetic smile with ceramic laminates through a minimally invasive approach. Case report: Female patient, attended the dental clinic reporting dissatisfaction with the appearance of her smile. During the evaluation, extensive restorations in composite resin were observed in the incisors in the anterosuperior region, with unsatisfactory proportion, amount, shape and texturing, which together compromised the aesthetic zone of the smile. Faced with this, after extra and intraoral photographs, the proposed treatment was the restoration with the ceramic laminates in units 1.2 to 2.2. Thus, waxing and mock-up were performed, which served as guidance for the dental preparations, then they were molded and restorations fabricated in the IPS e.max system. After shade and adaptation proof, the preparations and the inner ceramic laminates surface were treated and cemented with light-cured resin cement. Conclusion: Adopting a conservative approach, the treatment plan carried out through restoration with ceramic laminates on the upper anterior teeth allowed the recovering function and aesthetics, reproducing characteristics of natural teeth and promoting balance, joviality and harmony in the smile(AU)

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It is unknown whether HTLV-1/2 prevalence has been stable or changing with time in Brazil. We present a 10-year (2007-2016) analysis of HTLV-1/2 infection in first-time blood donors from four blood banks in Brazil. The Brazilian blood centers participating in this multicenter Recipient Epidemiology and Donor Evaluation Study (REDS) are located in Recife in the Northeast and in São Paulo, Rio de Janeiro and Belo Horizonte located in the Southeast of the country. A previous REDS study using the same database from 2007 to 2009 showed that the prevalence per 100,000 donors was 222 in Recife, 83 in Belo Horizonte and 101 in São Paulo. From 2007 to 2016, HTLV-1/2 prevalence was calculated by year, blood center and birth cohort. Covariates included age, gender, schooling, self-reported skin color and type of donation. From 1,092,174 first-blood donations, in the general analysis, HTLV-1/2 infection predominated in females, donors over 50 years of age, black skin color and less educated. The average prevalence was 228 per 100,000 donors in Recife, 222 in Rio de Janeiro, 104 in Belo Horizonte and 103 in São Paulo. In the 10-year analysis, HTLV-1/2 prevalence was stable, but a trend was observed toward an increase in HTLV-1/2 infection among younger people (p < 0.001), males (p = 0.049), those with white skin color (p < 0.001), and higher education (p = 0.014). Therefore, this 10-year surveillance of the infection showed stable HTLV-1/2 prevalence overall but a trend toward increased prevalence among the younger and more educated donors despite Brazilian policies to control sexually transmitted infections being in place for more than 10 years.

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The study of pollution effects in the marine environment has become important in recent decades, and the exposure to simultaneous pollutants has become especially relevant. Indeed, the study of key organisms, such as ecosystem engineers, can show a broader view of the effects of pollutants. In this context, we evaluate in situ the effects of a short (7-day) pollution pulse of combined solutions of heavy metals and polycyclic aromatic hydrocarbons (PAHs) (Cu + PAHs, Cd + PAHs, Cu + Cd, and Cu + Cd + PAHs) on the development and morphological features of Macrocystis pyrifera sporophytes over a period of 90 days. Additionally, we determined the effects on the community structure associated with this kelp. This study evidenced a smaller number of blades and a decreased size of blades and holdfasts, as well as the death of individuals exposed to a secondary mix of metals (Cu + Cd) and a tertiary mix of pollutants (Cu + Cd + PAHs). Regarding the effects on the accompanying fauna, low richness and diversity were registered. M. pyrifera grazers, according to the mixture of pollutants, were either absent or diminished. These results show that the pulse of contamination in the early stages of M. pyrifera negatively affects its development and morphometry, as well as its role as an ecosystem engineer, due to a negative alteration in the species composition.

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Introdução: É crescente a busca por tratamentos estéticos nas clínicas odontológicas, dentre esta demanda, encontra-se soluções reabilitadoras para casos de diastemas e incisivos laterais superiores com morfologia conoide, pois são tidos como fatores antiestéticos. Objetivo: Apresentar uma abordagem interdisciplinar no tratamento de diastemas e unidades dentárias com morfologia conoide. Relato de caso: O paciente procurou atendimento odontológico especializado para reabilitação provisória da estética e função das unidades anterossuperiores durante finalização do tratamento ortodôntico e posteriormente, após conclusão da ortodontia, a reabilitação final. Durante o exame clínico foram observados incisivos laterais superiores com morfologia conoides, ângulo incisal amplo entre os incisivos centrais, ausência de guia canina e diastemas. Após registro fotográfico, foram realizadas as restaurações provisórias. Finalizado o tratamento ortodôntico, o plano de tratamento consistiu na reabilitação através das facetas semidiretas em resina composta. Após moldagem anatômica, enceramento e clareamento dentário, foram realizados os preparos dentários e moldagem funcional, sendo a seguir realizado o ensaio restaurador. Sobre o modelo, foram confeccionadas as facetas em resina composta e realizado o acabamento inicial. Para a fase de cimentação priorizou-se a utilização de condicionamento ácido seletivo para esmalte, cimento fotopolimerizado e uso de polimerizador de alta potência. Após a cimentação foram realizados os ajustes oclusais e acabamento final, com texturização da superfície, seguido pelo polimento. Conclusão: O tratamento realizado evidenciou a importância do tratamento ortodôntico associado a Odontologia Restauradora para o restabelecimento da estética e função, obtendo resultados que melhoraram as relações entre dentes, posicionamento dos lábios, harmonia e equilíbrio dental e facial(AU)

Introduction: There is a growing demand for aesthetic treatments in dental clinics, among this demand, there are rehabilitation solutions for cases of diastemas and upper lateral incisors with conoidmorphology, as they are considered as anti-aesthetic factors. Objective: Present an interdisciplinary approach in the treatment of diastemas and dental units with conoid morphology. Case report: The patient sought specialized dental care for provisional rehabilitation of the aesthetics and function of the anterosuperior units during the finalisation of the orthodontic treatment and later, after completion of orthodontics, the final rehabilitation. During the clinical examination, upper lateral incisors with conoid morphology, wide incisal angle between the central incisors, absence of canine guide and diastemas were observed. After photographic register, provisional restorations were performed. After orthodontic treatment was completed, the treatment plan consisted of rehabilitation through semi-direct facets in composite resin. After anatomical moulding, waxing and tooth whitening, dental preparations and functional mouldingwere performed, hereinafter the restorative test was performed. In this connection,facets were made in composite resin and the initial finishing was carried out. For the cementation phase, priority was given to the use of selective acid conditioning for dental enamel, photopolymerized cement and the use of high-power polymerizer. After cementation, occlusal adjustments and finishing touch were performed with surface texturing, followed by polishing. Conclusion: The treatment performed showed the importance of orthodontic treatment associated with restorative dentistry for the restorationof aesthetics and function, obtaining results that improved the relationship between teeth, positioning of the lips, harmony and dental and facial balance(AU)

Introducción: Crece la búsqueda de tratamientos estéticos en las clínicas dentales, entre esta demanda se encuentran las soluciones rehabilitadoras para casos de diastema e incisivos laterales superiores con morfología conoide, por considerarse factores antiestéticos. Objetivo: Presentar un abordaje interdisciplinario en el tratamiento del diastema y unidades dentales con morfología conoide. Caso clínico: El paciente buscó atención odontológica especializada para la rehabilitación temporal de laestética y función de las unidades anterosuperiores durante la finalización del tratamiento de ortodoncia y posteriormente, trasla finalización de la ortodoncia, la rehabilitación final. Durante el examen clínico se observaron incisivos laterales superiores con morfología conoide, amplio ángulo incisal entre los incisivos centrales, ausencia de guía canina y diastema. Después del registro fotográfico, se realizaron restauraciones provisionales. Una vez finalizado el tratamiento de ortodoncia, el plan detratamiento consistió en la rehabilitación mediante carillas de resina compuesta semidirectas. Después del moldeado anatómico, encerado y blanqueamiento dental, se realizaron las preparaciones dentales y el moldeado funcional, seguido de la prueba restauradora.Sobre el modelo, las carillas se realizaron en resina compuesta y se realizó el acabado inicial. Para la fase de cementación se priorizó el uso de grabado ácido selectivo para esmalte, cemento fotopolimerizable y el uso de un polimerizador de alta potencia. Después de la cementación, se realizaron ajustes oclusales y acabado final, con texturizado de la superficie, seguido de pulido. Conclusión: El tratamiento realizado mostró la importancia del tratamiento de ortodoncia asociado a la odontología restauradora para la restauración de la estética y función, obteniendo resultados que mejoraron la relación entre los dientes, la posición de los labios, la armonía y el equilibrio dental y facial(AU)

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Animal models are widely used for studying diabetes in translational research. However, methods for induction of diabetes are conflicting with regards to their efficacy, reproducibility and cost. A comparison of outcomes between the diabetic models is still unknown, especially full-term pregnancy.To understand the comparison, we analyzed the streptozotocin (STZ)-induced diabetes at three life-different moments during the neonatal period in Sprague-Dawley female rats: at the first (D1), second (D2) and fifth (D5) day of postnatal life. At adulthood (90 days; D90), the animals were submitted to an oral glucose tolerance test (OGTT) for diabetic status confirmation. The diabetic and control rats were mated and sacrificed at full-term pregnancy for different analyses. Group D1 presented a higher mortality percentage after STZ administration than groups D2 and D5. All diabetic groups presented higher blood glucose levels as compared to those of the control group, while group D5 had higher levels of glycemia compared with other groups during OGTT. The diabetic groups showed impaired reproductive outcomes compared with the control group. Group D1 had lower percentages of mated rats and D5 showed a lower percentage of a full-term pregnancy. Besides that, these two groups also showed the highest percentages of inadequate fetal weight. In summary, although all groups fulfill the diagnosis criteria for diabetes in adult life, in our investigation diabetes induced on D5 presents lower costs and higher efficacy and reproducibility for studies involving diabetes-complicated pregnancy.

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