RESUMO
This study evaluated the efficiency of prostaglandin F2α (PGF) to hasten ovulation in weaned sows. In experiment I, weaned sows detected in estrus (0 h) received: no hormone (Control; n = 56); 0.5 mg PGF IM at 0 h and 2 h (PGF0; n = 56); or 0.5 mg PGF IM at 24 h and 26 h (PGF24; n = 55). In experiment II, weaned sows that did not express estrus signs until 72 h after weaning (0 h) were assigned to: no hormone (Control; n = 45); 10 µg buserelin acetate IM at 0 h (Buserelin; n = 43); 0.5 mg PGF IM at 34 h and 36 h (PGF; n = 44); or 10 µg buserelin acetate IM at 0 h plus 0.5 mg PGF IM at 34 h and 36 h (Buserelin + PGF; n = 45). In experiment I, no effect of PGF on the interval treatment onset to ovulation was observed (P > 0.05), and no treatment effect was observed on the relative or cumulative proportion of females that ovulated post-treatment onset (P > 0.05). In experiment II, treatment onset to ovulation interval was shorter for Buserelin group than for PGF group (P < 0.05), and a higher cumulative percentage of Buserelin treated sows ovulated up to 48 h compared to PGF and Control groups (P < 0.01), with no differences from Buserelin + PGF. Treatments did not affect total number of piglets born in both experiments (P > 0.05). In conclusion, PGF did not hasten ovulation timing or affect litter size in weaned sows.
Assuntos
Busserrelina , Dinoprosta , Ovulação , Animais , Feminino , Dinoprosta/farmacologia , Dinoprosta/administração & dosagem , Suínos/fisiologia , Ovulação/efeitos dos fármacos , Ovulação/fisiologia , Busserrelina/farmacologia , Busserrelina/administração & dosagem , Desmame , Indução da Ovulação/veterinária , Indução da Ovulação/métodosRESUMO
Objetivou-se investigar a eficiência dos meios TCM 199 e PBS na conservação de folículos pré-antrais(FOPA) felinos in situ a 4ºC por 24 h. Foram utilizados 10 pares de ovários de gatas Felis catus, sendo a camadacortical fracionada e submetida a três diferentes tratamentos: controle, T1 (fragmentos conservados em TCM199) e T2 (fragmentos conservados em PBS). Realizou-se a preparação histológica do grupo controle, sendo T1e T2 mantidos a 4ºC, por 24 h, em caixa isotérmica. Ao final dos tratamentos, os FOPA foram classificados emprimordiais, primários e secundários, e os oócitos classificados em normais ou degenerados morfologicamente. Aavaliação histológica constatou que os FOPA do controle (63%) tiveram maior integridade morfológica (P > 0,05)quando comparados ao T2 (55%), sendo semelhantes ao T1 (59%). Não houve diferença (P < 0,05) entreT1(59%) e T2 (55%). Os folículos primordiais mantiveram maior integridade morfológica quando comparadosaos folículos em desenvolvimento, independentemente do tratamento. Com base nesses resultados, pode-seconcluir que é possível a manutenção da integridade morfológica de FOPA em ovários de gatas mantidos a 4ºCpor 24 h, utilizando o TCM 199 e que os folículos primordiais têm maior viabilidade, quando comparados aosfolículos em desenvolvimento.(AU)
The aim of this study was to investigate the efficiency of TCM 199 and PBS mediums in the conservationof feline preantral follicles (PAF) in situ at 4°C for 24 h. Ten pairs of ovaries of Felis catus cats were used, inwhich the cortical layer was fractioned and submitted to three different treatments: Control, T1 (fragmentspreserved in TCM 199) and T2 (fragments preserved in PBS). Histological preparation was carried out in thecontrol group, while T1 and T2 were kept at 4°C for 24 h in an isothermal box. After histological preparation ofControl, T1 and T2 the PAF were classified as primordial, primary and secondary and the morphology of theoocyte as normal and degenerated. Histological evaluation found that the PAF control group (63%) had a highermorphological integrity (P > 0.05) compared to T2 (55%), which was similar to T1 (59%). However, there wasno difference (P < 0.05) between T1 (59%) and T2 (55%) treatments. Primordial follicles maintained greatermorphological integrity when compared to developing follicles, regardless of the treatment. Based on theseresults, it can be concluded that it is possible to maintain the morphological integrity of PAF in ovaries of catstransported at 4°C for 24 h using TCM 199 and that primordial follicles have a higher viability compared todeveloping follicles.(AU)
Assuntos
Animais , Feminino , Gatos , Gatos/embriologia , Gatos/fisiologia , Ovário/citologiaRESUMO
Objetivou-se investigar a eficiência dos meios TCM 199 e PBS na conservação de folículos pré-antrais(FOPA) felinos in situ a 4ºC por 24 h. Foram utilizados 10 pares de ovários de gatas Felis catus, sendo a camadacortical fracionada e submetida a três diferentes tratamentos: controle, T1 (fragmentos conservados em TCM199) e T2 (fragmentos conservados em PBS). Realizou-se a preparação histológica do grupo controle, sendo T1e T2 mantidos a 4ºC, por 24 h, em caixa isotérmica. Ao final dos tratamentos, os FOPA foram classificados emprimordiais, primários e secundários, e os oócitos classificados em normais ou degenerados morfologicamente. Aavaliação histológica constatou que os FOPA do controle (63%) tiveram maior integridade morfológica (P > 0,05)quando comparados ao T2 (55%), sendo semelhantes ao T1 (59%). Não houve diferença (P < 0,05) entreT1(59%) e T2 (55%). Os folículos primordiais mantiveram maior integridade morfológica quando comparadosaos folículos em desenvolvimento, independentemente do tratamento. Com base nesses resultados, pode-seconcluir que é possível a manutenção da integridade morfológica de FOPA em ovários de gatas mantidos a 4ºCpor 24 h, utilizando o TCM 199 e que os folículos primordiais têm maior viabilidade, quando comparados aosfolículos em desenvolvimento.
The aim of this study was to investigate the efficiency of TCM 199 and PBS mediums in the conservationof feline preantral follicles (PAF) in situ at 4°C for 24 h. Ten pairs of ovaries of Felis catus cats were used, inwhich the cortical layer was fractioned and submitted to three different treatments: Control, T1 (fragmentspreserved in TCM 199) and T2 (fragments preserved in PBS). Histological preparation was carried out in thecontrol group, while T1 and T2 were kept at 4°C for 24 h in an isothermal box. After histological preparation ofControl, T1 and T2 the PAF were classified as primordial, primary and secondary and the morphology of theoocyte as normal and degenerated. Histological evaluation found that the PAF control group (63%) had a highermorphological integrity (P > 0.05) compared to T2 (55%), which was similar to T1 (59%). However, there wasno difference (P < 0.05) between T1 (59%) and T2 (55%) treatments. Primordial follicles maintained greatermorphological integrity when compared to developing follicles, regardless of the treatment. Based on theseresults, it can be concluded that it is possible to maintain the morphological integrity of PAF in ovaries of catstransported at 4°C for 24 h using TCM 199 and that primordial follicles have a higher viability compared todeveloping follicles.