RESUMO

The potential value of PCRs in the species-specific diagnosis of have been investigated, using samples of T. saginata and T. solium from different geographical areas. The PCRs examining inter-species differences were based on the sequence of the HDP2 DNA fragment, specific for T. saginata/T. solium, and the sequence of the rDNA internal transcribed spacer 1 and spacer 2 (ITS-1 and ITS-2). This PCR analysis of DNA isolates confirmed morphologic diagnosis and allowed the speciation of samples too small or fragmented for morphologic identification, with clear and consistent inter-species differences between T. saginata (twenty-two) and T. solium (three) geographical isolates. Possible intra-species genomic variability, within these species, was similarly studied through analysis of PCR amplification products (PCR-RFLP) and only encountered one exceptional T. saginata isolate from Kenya, which yielded a unique PCR-RFLP pattern, different from T. saginata DNA of Mexican (one sample) and Spanish (seven samples) origin.

Assuntos

DNA de Helmintos/genética , Reação em Cadeia da Polimerase/métodos , Taenia/crescimento & desenvolvimento , Teníase/diagnóstico , Animais , Anticorpos Antibacterianos/sangue , Bovinos , DNA de Helmintos/química , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Diagnóstico Diferencial , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Quênia , Masculino , México , Polimorfismo de Fragmento de Restrição , Espanha , Especificidade da Espécie , Suínos , Taenia/química , Taenia/genética , Teníase/parasitologia