RESUMO
Most zinc studies show its benefits or changes that coincide with its deficiency, but some have reported damages by supplements. In this work, the effects of zinc in different cell lines (U-937, human monocytes, and murine bone marrow cells) were analyzed. The cells were put in their specific culture medium either alone or with a stimulant [1-phorbol 12-myristate 13-acetate (PMA) for U-937 and monocytes, granulocyte macrophage colony stimulating factor (GM-CSF) for bone marrow cells]. These preparations, with or without zinc (0.05 to 1.0 mM), were incubated and microscopically analyzed on days 3, 9, and 11. The viability of all cells cultivated with 0.05 and 0.1 mM of zinc was similar to that of the controls without zinc (90%). With 1.0 mM of zinc, the viability diminished (p < 0.005) to 80% in U-937 and to 50% in monocytes and bone marrow cells; the number of cells increased in the three lines, but there was no differentiation. We conclude that the effects observed with different doses of zinc vary not only among the different species but also according to the time the cells were exposed to the metal. The same doses of zinc can have either a stimulatory or an inhibitory effect.
Assuntos
Diferenciação Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Zinco/farmacologia , Animais , Células da Medula Óssea/efeitos dos fármacos , Humanos , Camundongos , Monócitos/efeitos dos fármacos , Células U937Assuntos
Agressão , Antipsicóticos/uso terapêutico , Doenças dos Símios Antropoides/tratamento farmacológico , Gorilla gorilla , Haloperidol/uso terapêutico , Transtornos Mentais/veterinária , Agressão/efeitos dos fármacos , Animais , Antipsicóticos/farmacologia , Ansiedade/tratamento farmacológico , Feminino , Haloperidol/farmacologia , Transtornos Mentais/tratamento farmacológico , Comportamento Autodestrutivo/tratamento farmacológico , Resultado do TratamentoRESUMO
In the antique Chinese culture, it was already known about opioide for therapeutic usage. On 1975, Hughes et al., identificated two endogen pentapetides with potent opiate activity Leu-enkephalin and Met-enkephalin, ever since beta-Endorphin have been considered involucrated in many biological functions. beta-Endorphin have found in testis, seminal vesicle and prostate in different species. It has been observed a paracrine effect between in Leydig and Sertoli cells and inhibit the basal release of LH in man by inhibiting its pulsatile discharge. In this paper is discussed, in a general way, localization and production sites of beta-Endorphin especially their participation in male reproductive tract-physiology. Finally, it is mentioned the experimental methodology to identify and quantify beta-Endorphin in blood plasma and semen.