RESUMO
The in vitro effect of progesterone in T. canis larvae on their enlargement and motility were evaluated, together to the possible presence of progesterone receptors (PRs). T. canis larvae were cultured in RPMI-1640 with different concentrations of progesterone (0, 20, 40, 80, 400 and 800 ng/mL). Enlargement and increases in motility were dependent on the concentration only from 0 to 80 ng/mL (p < 0.05). The mean percentage of PR + cells in newly obtained larvae as measured by flow cytometry was 8.16 ± 0.4. The number of PR + cells increased depending on concentration from 0 to 80 ng/mL (p < 0.001). Cells obtained from larvae stimulated at any of the studied hormone concentrations showed greater mean fluorescence intensity when compared to non-stimulated cells. Additionally, the expression and location of PR + cells were determined in the larvae. The sequence of an amplicon (420-bp) obtained by PCR from T. canis larvae showed 100% homology with a gene fragment that codes for the PR of the dog. PR + cells were immunolocated using confocal microscopy in the intestinal region of the larvae that had been recently obtained. The results of this study show that T. canis larvae can recognize and respond to the presence of progesterone through a molecule possibly able to bind it. Since we previously observed a similar response to prolactin, we suggest that both hormones could participate sequentially in the reactivation of T. canis larvae in pregnant bitches.
Assuntos
Progesterona/farmacologia , Progestinas/farmacologia , Receptores de Progesterona/efeitos dos fármacos , Toxocara canis/efeitos dos fármacos , Animais , Sequência de Bases , DNA de Helmintos/química , DNA de Helmintos/isolamento & purificação , Cães , Feminino , Citometria de Fluxo , Intestinos/parasitologia , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Larva/fisiologia , Camundongos , Microscopia Confocal , Movimento/efeitos dos fármacos , Reação em Cadeia da Polimerase , Receptores de Progesterona/análise , Receptores de Progesterona/genética , Receptores de Progesterona/metabolismo , Toxocara canis/crescimento & desenvolvimento , Toxocara canis/fisiologiaRESUMO
Androgens have been shown to exert a cysticidal effect upon Taenia crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps, parasite extracts were resolved into approximately 130 proteins distributed in a molecular weight range of 10-250 kDa and isoelectrical point range of 3-10. The resultant proteomic pattern was analysed, and significant changes were observed in response to T4 and DHT. Based on our experience with electrophoretic patterns and proteomic maps of cytoskeletal proteins, alteration in the expression of isoforms of actin, tubulin and paramyosin and of other proteins was assessed. Considering that androgens may exert their biological activity in taeniids through the non-specific progesterone receptor membrane component (PGRMC), we harnessed bioinformatics to propose the identity of androgen-regulated proteins and establish their hypothetical physiological role in the parasites. These analyses yield a possible explanation of how androgens exert their cysticidal effects through changes in the expression of proteins involved in cytoskeletal rearrangement, dynamic vesicular traffic and transduction of intracellular signals.
Assuntos
Androgênios/farmacologia , Morte Celular , Proteoma , Taenia/efeitos dos fármacos , Taenia/fisiologia , Actinas/genética , Animais , Biologia Computacional , Cisticercose/patologia , Cysticercus/efeitos dos fármacos , Cysticercus/fisiologia , Citoesqueleto/efeitos dos fármacos , Citoesqueleto/genética , Di-Hidrotestosterona/farmacologia , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Receptores de Progesterona/genética , Testosterona/farmacologia , Tropomiosina/genética , Tubulina (Proteína)/genéticaRESUMO
We evaluated the direct effects of progesterone on the morphology, maturation and behavior of Haemonchus contortus larvae in vitro. The presence and location of possible progesterone receptors in these larvae were also determined. The addition of 8ng/mL of progesterone to larval cultures over 10days reduced larval enlargement, while the addition of 160ng/mL of the hormone increased the enlargement. Up to 62% and 65% of the H. contortus larvae molted from third-stage larvae (L3) to fourth-stage larvae (L4) when cultured in RPMI-1640 media without hormone for 5 and 10days, respectively. The addition of different progesterone concentrations (1, 8, 16, 80 and 160ng/mL) to the larval cultures significantly inhibited the molting process within the same periods. The addition of 8ng/mL or higher progesterone concentrations to the cultures significantly increased larval motility (p<0.05) compared with unstimulated larvae. Flow cytometry showed the expression of progesterone receptors (P4-R) in 15% of the cells from newly isolated H. contortus larvae. When the larvae were cultured for 5days in the presence of the hormone, the percentage of P4-R+ cells remained the same. In contrast, unstimulated larvae showed a significant reduction in the number of P4-R+ cells. Using confocal microscopy, a greater concentration of P4-Rs was immunolocated in the anterior portion of the alimentary tract of the larvae, suggesting that the cells in this region are targeted by the hormone. The results of the present study show that H. contortus larvae have possible P4-Rs and respond to this hormone by inhibiting their molting process, thereby suggesting the participation of progesterone in the larval arrest phenomenon.
Assuntos
Haemonchus/efeitos dos fármacos , Progesterona/administração & dosagem , Progestinas/administração & dosagem , Animais , Relação Dose-Resposta a Droga , Haemonchus/genética , Haemonchus/crescimento & desenvolvimento , Haemonchus/metabolismo , Larva/efeitos dos fármacos , Larva/genética , Larva/crescimento & desenvolvimento , Larva/metabolismo , Muda/efeitos dos fármacos , Receptores de Progesterona/genética , Receptores de Progesterona/metabolismoRESUMO
The in vitro effect of prolactin (PRL) on the growth and motility of Toxocara canis larvae was assessed. Additionally, the expression and location of prolactin receptors (PRL-Rs) were determined in the larvae. Larvae of T. canis were incubated with different concentrations of PRL for different periods of time. The stimulated larvae accelerated their enlargement and increased their motility. The mean percentage of PRL-R+ cells in non-stimulated larvae, measured by flow cytometry was 7.3±0.3%. Compared with non-stimulated larvae, the mean fluorescence intensity (p<0.05) increased in larvae incubated with 40ng/mL of PRL for 10 days. A 465-bp length fragment was amplified from larvae gDNA by PCR. The sequence of this fragment showed 99% similarity with the gene fragment that codes for the PRL-R of the domestic dog. A high concentration of PRL-Rs was immune-located in the posterior region of the larval intestine; therefore, the intestinal cells in this region were most likely the targets for this hormone. Based on these results, PRL-Rs were identified in T. canis larvae, and the in vitro stimulation with PRL increased the number of these receptors, accelerated the growth and modified the activity of larvae. All of the above suggest that T. canis larvae are evolutionarily adapted to recognize the PRL of their definitive host and furthermore might explain the reactivation of tissue-arrested larvae during the gestation of bitches, which does not occur in gestating females of other species.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Prolactina/farmacologia , Receptores da Prolactina/metabolismo , Toxocara canis/efeitos dos fármacos , Toxocara canis/fisiologia , Toxocaríase/parasitologia , Animais , Hormônios/farmacologia , Técnicas In Vitro , Larva , Toxocara canis/genética , Toxocara canis/crescimento & desenvolvimentoRESUMO
During the helminth infections, the immune system tends to be modulated by host's sex hormones. Actually, many studies show the reciprocal relationship between sex steroids, the immune system and the elimination or establishment of helminth parasites. Is well known that innate immune response determines the type of adaptive immune response, so the effects in the innate immune response by hormones may affect subsequent adaptive immunity. The sex steroids as estrogens, progesterone and testosterone regulate growth, differentiation, survival and function of many cell types that could be involved in process like homeostasis and immunity, but also have a direct effect on the helminthes, that may probably be mediated by specific receptors on these parasites. Sex steroids, parasites and immunity are closely connected, and their interconnection is involved in the maintenance of elimination or establishment of helminthes in an immunocompetent host. For that reason, understanding the action's mechanisms of sex steroids on immune cells and its direct effect on helminth parasites is important for further progress in the development of novel therapies for chronic helminth diseases associated to immune dysregulation. In this review, we will describe the effects of sex steroids on the immune response during helminth infections as well as the direct effect in these parasites, and the possible implications of these effects on the incidence of several helminth infections.
Assuntos
Hormônios Esteroides Gonadais/imunologia , Helmintíase/imunologia , Helmintos/fisiologia , Interações Hospedeiro-Parasita , Imunidade Inata , Animais , Helmintíase/parasitologia , Helmintos/imunologia , Humanos , Sistema Imunitário/imunologia , Sistema Imunitário/parasitologia , ImunidadeRESUMO
Neurocysticercosis (NCC) is a disease of the central nervous system that is considered a public health problem in endemic areas. The definitive diagnosis of this disease is made using a combination of tools that include imaging of the brain and immunodiagnostic tests, but the facilities for performing them are usually not available in endemic areas. The immunodiagnosis of NCC is a useful tool that can provide important information on whether a patient is infected or not, but it presents many drawbacks as not all infected patients can be detected. These tests rely on purified or semipurified antigens that are sometimes difficult to prepare. Recent efforts have focused on the production of recombinant or synthetic antigens for the immunodiagnosis of NCC and interesting studies propose the use of new elements as nanobodies for diagnostic purposes. However, an immunodiagnostic test that can be considered as "gold standard" has not been developed so far. The complex nature of cysticercotic disease and the simplicity of common immunological assumptions involved explain the low scores and reproducibility of immunotests in the diagnosis of NCC. Here, the most important efforts for developing an immunodiagnostic test of NCC are listed and discussed. A more punctilious strategy based on the design of panels of confirmed positive and negative samples, the use of blind tests, and a worldwide effort is proposed in order to develop an immunodiagnostic test that can provide comparable results. The identification of a set of specific and representative antigens of T. solium and a thorough compilation of the many forms of antibody response of humans to the many forms of T. solium disease are also stressed as necessary.
Assuntos
Antígenos de Helmintos , Testes Imunológicos , Neurocisticercose/diagnóstico , Taenia solium/imunologia , Animais , Especificidade de Anticorpos , Antígenos de Helmintos/análise , Antígenos de Helmintos/imunologia , Encéfalo/patologia , Humanos , Neurocisticercose/parasitologia , Neuroimagem , Sensibilidade e Especificidade , Taenia solium/isolamento & purificaçãoRESUMO
The physiological interactions during the course of any immune response are complex. Infection induces antigen-specific recognition by the immune system, which is consequently charged with the responsibility of marshalling the appropriate effector responses necessary to destroy the pathogen, or at the very least inhibit its progression. Obviously, the immune system should accomplish this while minimizing collateral damage to the host or it risks, winning a Pyrrhic victory. As our understanding of the neuroendocrine system grows, it has become increasingly clear that this complex network of neurotransmitters, hormones and cytokines plays an important role in mediating immunity. Schistosomes present an especially complex relationship between pathogen and these physiological systems, with hormonally dependent host factors such as sex and age correlated with parasite success. In this report, we review the current literature on sex and age associations between infection and progression of disease. We then follow with a discussion on interactions between the host neuroendocrine and immune systems. We also speculate on strategies to apply this knowledge to novel treatment strategies. Results argue for a complex network comprising the immune, endocrinological and nervous systems of both host and schistosome in the regulation of the plural outcomes of infection.
Assuntos
Interações Hospedeiro-Parasita , Sistemas Neurossecretores/imunologia , Esquistossomose/imunologia , Fatores Etários , Animais , Feminino , Humanos , Masculino , Esquistossomose/epidemiologia , Fatores SexuaisRESUMO
In this special issue of Parasite Immunology, the reader will find reviewed some of the hottest topics in immunoparasitology, with emphasis on the most studied parasite species in Mexico. For instance, the immunological conditions that appear favorable for the survival or destruction of the parasite in the intermediate and definitive hosts in cysticercosis, as well as the use of immunodiagnostic tests in epidemiological/intervention studies are discussed in two different articles. The role that alternatively activated macrophages plays in modulating host immunity is also discussed, while in the field of Leishmaniasis, the reader will find reviewed the role that CD8+ T cells play in the host defense during the human infection. The role that antibodies may play as biomarkers of protective or pathological cellular immune events in Toxoplasma gondii infections, as well as the new insights about the regulation of the inflammatory immune response by the cytokine/chemokine network in amebiasis, are topics reviewed. The use that TSL-1 antigens may have in the development of more sensitive and specific diagnosis of human and animal trichinellosis as well as the role that the neuroimmunoendocrine network plays during schistosomiasis are also presented. We hope that our readers will find fascinating and enticing, the first ever Special Issue devoted to Mexican Immunoparasitology.
Assuntos
Amebíase/imunologia , Cisticercose/imunologia , Leishmaniose/imunologia , Esquistossomose/imunologia , Toxoplasmose/imunologia , Triquinelose/imunologia , Animais , Cisticercose/diagnóstico , Cisticercose/epidemiologia , Humanos , México/epidemiologia , Toxoplasmose/patologia , Triquinelose/diagnósticoRESUMO
In chronically infected BALBc/AnN male mice, Taenia crassiceps cysticercosis induces changes in the host's sex steroids hormone that lead to their estrogenization and deandrogenization, with possible repercussions on their susceptibility to infections. Here reported are the serum steroid levels in free range cysticercotic male boars. Therefore, the possible effects of Taenia solium cysticerci over the pig steroid levels were evaluated. Herein are described the sex steroids and cortisol levels of non-cysticercotic (n=25) and cysticercotic (n=22) adult boars, as diagnosed by tongue inspection, all free-ranging in a typical village of an endemic rural area in Mexico. A significant reduction of testosterone (P=0.022) and a likely one of 17beta-estradiol (P=0.08) levels were found in the cysticercotic boars in comparison with those non-cysticercotic, whilst no significant differences in the cortisol and DHEA levels were detected. Serum levels of specific antibodies did not correlate with infection nor with the levels of any of the hormones measured. Results suggest that T. solium cysticercosis significantly affects the hormonal status of its porcine host independently of their antibody response.
Assuntos
Cisticercose/veterinária , Hormônios/sangue , Doenças dos Suínos/fisiopatologia , Taenia solium/fisiologia , Animais , Anticorpos Anti-Helmínticos/sangue , Cisticercose/fisiopatologia , Desidroepiandrosterona/sangue , Estradiol/sangue , Hidrocortisona/sangue , Masculino , México , Projetos Piloto , Suínos , Testosterona/sangueRESUMO
A review of current literature on mammalian hosts' sexual dimorphism (SD) in parasitic infections revealed that (1) it is a scarcely and superficially studied biological phenomenon of considerable significance for individual health, behavior, and lifestyles and for the evolution of species; (2) there are many notable exceptions to the rule of a favorable female bias in susceptibility to infection; (3) a complex network of molecular and cellular reactions connecting the host's immuno-neuroendocrine systems with those of the parasite is responsible for the host-parasite relationship rather than just an adaptive immune response and sex hormones; (4) a lack of gender-specific immune profiles in response to different infections; (5) the direct effects of the host hormones on parasite physiology may significantly contribute to SD in parasitism; and (6) the need to enrich the reductionist approach to complex biological issues, like SD, with more penetrating approaches to the study of cause-effect relationships, i.e., network theory. The review concludes by advising against generalization regarding SD and parasitism and by pointing to some of the most promising lines of research.