RESUMO
Some species of the genus Anastrepha (Diptera: Tephritidae) are successfully managed by matching the sterile insect technique with parasitoid releases. Such strategies used in integrated pest management can be implemented only where insect mass-rearing programs are feasible. In this study, we show the process of domestication, rearing technology and quality control data obtained from 54 generations of Anastrepha sp.1 aff. fraterculus (Wiedemann, 1830) kept under fully artificial conditions. Eggs were collected by an artificial oviposition panel consisting of one side of the cage made of blue voile fabric externally covered with a thin layer of silicon rubber. They were then air-bubbled in water at 25 ºC for 48 h before seeding. Larvae were reared on the regular laboratory artificial diet with 66 % of agar reduction turning over a semi-liquid diet, which reduced costs and improved insect quality. The adult and larval diets were composed of local ingredients including hydrolyzed yeast. When large-scale production of this fly is contemplated, the critical stage is larval development. This system of artificial rearing for A. fraterculus sp.1 developed in Brazil, allows for the production of a large number of insects of excellent quality using local ingredients and less agar in diet composition than the original medium used for this species. By reducing the interval of egg collection, the system might be optimized in terms of insect yield and, therefore, meet the demands of A. fraterculus sp.1 with regard to integrated pest management purposes.
Assuntos
Dípteros , Moscas Domésticas , Tephritidae/crescimento & desenvolvimento , Tephritidae/embriologia , BrasilRESUMO
Some species of the genus Anastrepha (Diptera: Tephritidae) are successfully managed by matching the sterile insect technique with parasitoid releases. Such strategies used in integrated pest management can be implemented only where insect mass-rearing programs are feasible. In this study, we show the process of domestication, rearing technology and quality control data obtained from 54 generations of Anastrepha sp.1 aff. fraterculus (Wiedemann, 1830) kept under fully artificial conditions. Eggs were collected by an artificial oviposition panel consisting of one side of the cage made of blue voile fabric externally covered with a thin layer of silicon rubber. They were then air-bubbled in water at 25 ºC for 48 h before seeding. Larvae were reared on the regular laboratory artificial diet with 66 % of agar reduction turning over a semi-liquid diet, which reduced costs and improved insect quality. The adult and larval diets were composed of local ingredients including hydrolyzed yeast. When large-scale production of this fly is contemplated, the critical stage is larval development. This system of artificial rearing for A. fraterculus sp.1 developed in Brazil, allows for the production of a large number of insects of excellent quality using local ingredients and less agar in diet composition than the original medium used for this species. By reducing the interval of egg collection, the system might be optimized in terms of insect yield and, therefore, meet the demands of A. fraterculus sp.1 with regard to integrated pest management purposes.(AU)
Assuntos
Tephritidae/embriologia , Tephritidae/crescimento & desenvolvimento , Dípteros , Moscas Domésticas , BrasilRESUMO
The aim of this study was to find alternatives to reduce the cost of mass production of the South American fruit fly (A. fraterculus) by looking for locally available products as protein source in the diet of adults to replace the imported product without changing the quality parameters. Two yeast from a Brazilian company were evaluated. The quality parameters showed that the imported hydrolyzed yeast used in the adult diet could be perfectly replaced by the local products tested, with a reduction of over 80% of the cost of the diet. The quality of the produced insects remained the same and there were improvements in some quality parameters such as the volume of eggs produced, number of adults flying and longevity under the stress.
RESUMO
We studied the dispersal behavior and survival of sterile medfly males either treated or not with ginger root oil (GRO), in field conditions, in Petrolina-PE, northeast Brazil, from May 2006 to December 2007 in a sterile insect technique (SIT) program. The tsl strain Vienna 8 from the Ceratitis capitata Wied. (Diptera: Tephritidae), medfly, mass-rearing facility located in Juazeiro-BA, Brazil, was used. The results showed that sterile males either exposed or not to GRO exhibit similar dispersal behavior and postrelease survival. More than 60% of the sterile males, either treated or not treated with GRO, were recovered at a 25-m distance from the releasing point, approximately 20% at 50 m, and 5% in traps situated 100 m from the releasing point. Around 90% of the sterile males, exposed or not to GRO, were recovered 5 d after release of the sterile male individuals, whereas <1% were recovered after 11 d. Our results imply that ginger root oil can be used to treat sterile medfly males without interfering with their dispersal or survival in the field.