RESUMO
AIM: The aim of this study was to investigate the effects of acute and chronic systemic stress response on orthodontically induced root resorption. METHODS: Male Wistar rats were restrained during 1 hour a day by stress models of short (3 days) and long duration (40 days), while control group was not submitted to restraint (N.=10/group). The upper left first molars of all rats were moved mesially by a fixed orthodontic appliance exerting 50 g force upon insertion during the last 14 days of the experiment. Then, animals were killed for blood collection and mensuration of plasmatic corticosterone by radioimmunoassay; the tissues around mesial root of the first molar were processed for histological and histochemical techniques with tartrate-resistant acid phosphatase. The degree of root resorption and the number of odontoclasts were evaluated, being the contralateral side of each animal serving as its control (split-mouth design). RESULTS: The results revealed that the plasmatic levels of corticosterone were significantly higher in both the stressed groups than in the control one. There were no significant differences in the degree of root resorption and in the number of odontoclasts on the root between the 3 groups studied. CONCLUSIONS: These results indicate that systemic stress alone can not be considered a risk factor for root resorption induced by orthodontic tooth movement.
Assuntos
Aparelhos Ortodônticos/efeitos adversos , Reabsorção da Raiz/etiologia , Estresse Psicológico/fisiopatologia , Técnicas de Movimentação Dentária/efeitos adversos , Fosfatase Ácida/análise , Animais , Biomarcadores , Contagem de Células , Corticosterona/sangue , Isoenzimas/análise , Masculino , Dente Molar/enzimologia , Dente Molar/patologia , Desenho de Aparelho Ortodôntico , Osteoclastos/patologia , Ratos , Ratos Wistar , Restrição Física , Reabsorção da Raiz/sangue , Reabsorção da Raiz/patologia , Estresse Psicológico/sangue , Estresse Psicológico/etiologia , Fosfatase Ácida Resistente a Tartarato , Fatores de Tempo , Técnicas de Movimentação Dentária/métodosRESUMO
INTRODUCTION: It has been reported that anti-inflammatory drugs used for treatment of pain and discomfort related to orthodontic treatment could slow down tooth movement. However, the effect of these drugs on orthodontic root resorption is not well understood. OBJECTIVES: The aim of this study was to investigate whether the COX-2 inhibitor celecoxib offers some protection against orthodontically induced root resorption. DESIGN: Male Wistar rats were divided into four groups: Groups I and II were treated with saline and celecoxib (10 mg / kg), respectively for 3 days. Groups III and IV were treated with saline and celecoxib for 14 days. The upper left first molars of all rats were moved mesially for 14 days with 50 g of force. An area including the disto-apical aspect of the mesial root of the first molar was processed for histological and histochemical techniques with tartarate-resistant acid phosphatase (TRAP). OUTCOME MEASURE: The degree of root resorption was measured using an image analysis system with a grid-sheet superimposed in the root were resorption lacunae were counted. The number of TRAP-positive cells on the tooth root surface defined as odontoclasts were also evaluated. RESULTS: The results revealed that there were no significant differences in the degree of root resorption and in the number of odontoclasts on the root between the four groups studied. CONCLUSION: The short and long-term celecoxib administration did not suppress the root resorption in case of experimental orthodontic force application.
Assuntos
Inibidores de Ciclo-Oxigenase 2/uso terapêutico , Pirazóis/uso terapêutico , Reabsorção da Raiz/tratamento farmacológico , Reabsorção da Raiz/etiologia , Sulfonamidas/uso terapêutico , Técnicas de Movimentação Dentária/efeitos adversos , Animais , Celecoxib , Inibidores de Ciclo-Oxigenase 2/farmacologia , Análise do Estresse Dentário , Masculino , Osteoclastos/efeitos dos fármacos , Pirazóis/farmacologia , Distribuição Aleatória , Ratos , Ratos Wistar , Sulfonamidas/farmacologiaRESUMO
Os autores investigaram os efeitos da soluçäo anestésica Citocaína 3½ (pH 4,6) com Felipressina nas mais variadas situaçöes da clínica médica odontollógica e concluiram ser este produto o ideal para pacientes portadores de inflamaçäo, hipertensos, cardiopatas, diabéticos, idosos, gestantes e crianças