RESUMO
PURPOSE: Chronic alcoholism is a well-known risk factor for strongyloidiasis, in these patients the disease is potentially more severe, probably due to the breakdown of local protective barriers and immunosuppression caused by alcohol, which can lead to autoinfection and dissemination. The aim of this study was to evaluate multiple stool sampling and a specific parasitological assay agar plate culture (APC) for the diagnosis of Strongyloides stercoralis in alcoholics. METHODS: APC was compared to sedimentation technique (HPJ; Hoffman, Pons and Janer), as parasitological methods to detect S. stercoralis infection in alcoholic individuals. Three stool samples from 60 alcoholic and 60 non-alcoholic individuals were analyzed. RESULTS: S. stercoralis larvae were observed in 11 (18.3%) alcoholic individuals and 1 (1.7%) nonalcoholic individual (P = 0.0042). In view of the combined results, sensitivity for the APC method was 63.6% (CI 31.6-87.6%) with the first sample reaching 100% (CI 67.8-100%) after analyzing three fecal samples. The HPJ sensitivity was 36.4% (CI 12.4-68.4) in the first sample, reaching 72.7% (CI 39.3-92.7) after three samples analyzed. CONCLUSION: The present results suggest that in alcoholic patients, it is important to repeat stool sampling with specific techniques, especially using the APC method, to avoid misdiagnosis in cases that could evolve to disseminated strongyloidiasis.
Assuntos
Alcoólicos , Alcoolismo , Strongyloides stercoralis , Estrongiloidíase , Animais , Humanos , Estrongiloidíase/diagnóstico , Alcoolismo/diagnóstico , Fatores de Risco , FezesRESUMO
Strongyloidiasis is a human parasitosis that is considered a public health problem. Early diagnosis of this infection is extremely important in immunocompromised patients (i.e. subjects with alcoholism). This study aimed to evaluate anti-Strongyloides immunoglobulin G (IgG) and immunoglobulin A (IgA), assess levels of circulating immune complexes (IC) and determine IgG avidity in serum samples from alcoholic and nonalcoholic individuals. A total of 140 blood samples were collected from male individuals (70 alcoholic and 70 nonalcoholic subjects). Serum was obtained and analysed by enzyme-linked immunosorbent assay for IgG, IgA, IC detection and avidity determination. Anti-Strongyloides IgG was detected in 55.7% of alcoholic subjects and 32.8% nonalcoholics, while IC levels showed frequencies of 38.6% and 17.1% in these groups, respectively. Anti-Strongyloides IgA was lower among alcoholics (4.3%) than nonalcoholics (34.3%). Spearman's correlation coefficient reported a positive correlation between IgG, IC and IgA in alcoholic individuals and no correlation in nonalcoholics. The median avidity index was higher in alcoholics (83.8%) than nonalcoholic subjects (73.2%). In conclusion, this study shows that alcoholic subjects produced specific antibodies against S. stercoralis regardless of the possible immunosuppression caused by chronic alcoholism. Considering that alcoholics are more susceptible to the severe forms of strongyloidiasis, the implementation of immunological methods as a complementary approach to parasitological diagnostics (i.e. detection of IgG, IC and antibody avidity) appears to be an alternative method for early diagnosis in these individuals.
Assuntos
Alcoólicos , Anticorpos Anti-Helmínticos/sangue , Complexo Antígeno-Anticorpo/sangue , Estrongiloidíase/diagnóstico , Estrongiloidíase/imunologia , Adulto , Animais , Afinidade de Anticorpos , Estudos Transversais , Ensaio de Imunoadsorção Enzimática , Fezes/parasitologia , Humanos , Hospedeiro Imunocomprometido , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Masculino , Pessoa de Meia-Idade , Strongyloides stercoralis , Estrongiloidíase/sangueRESUMO
Estudando-se 45 alcoólatras sem hepatopatia clínica evidente, pode-se classificá-los em três grupos: 27% dos alcoólatras näo apresentaram nenhuma anormalidade clínica ou laboratorial; 38% apresentaram apenas alteraçöes laboratoriais (sendo que em 59% destes o único exame alterado foi a gamaglutamiltransferase); e 35% apresentaram hepatomegalia. O grupo com hepatomegalia teve média de ingesta etílica total significativamente superior em relaçäo à dos outros dois grupos. Destaca-se a importância, para avaliaçäo do fígado em alcoólatras, do uso dos seguintes parâmetros: ingesta etílica total, hepatomegalia e gamaglutamiltransferase