RESUMO
The possible role of histamine locally applied into the nucleus accumbens on exploratory behaviours measured in 'conflictive' and 'non-conflictive' environments was studied in adult male rats. It was assumed that in conflictive environments the brain mechanisms involved in processing incentive environmental clues (novelty) were interacting with mechanisms involved in the processing of fearful or 'anxiogenic' environmental clues. As a model of conflictive environment, the elevated asymmetric-plus maze (APM) was used. As a model of a non-conflictive environment, a modified holebroad enriched with an object (OVM) was used. The exploration score in any of the arms of the APM was considered an approximate index of exploratory motivation. The permanency score (non-exploratory behaviours) was considered an inverse approximate index of emotionality. Other variables such as the frequency of entries into any arm, the latency time and central activity were also measured. In the OVM, the general motor activity and head-dipping, vertical rearing and focalized exploration were measured. Results show that histamine in the APM had a dual effect. On the one hand, an increase of exploration was observed in those arms considered more 'anxiogenic'. On the other hand, a decrease in exploration occurred in one of the arms considered less 'anxiogenic'. No changes of permanency was observed in the 'anxiogenic' arms, and a decrease of permanency took place in the arms considered less 'anxiogenic'. In the OVM, histamine did not change the overall motor activity, but head-dipping was inhibited by the imidazolamine treatment. Histamine effects on exploration parameters were counteracted by pre-treatment with H1- and H2-histamine antagonists. Nevertheless, some behaviours were not blocked by the histamine receptor antagonists. The present results give support to the role of the nucleus accumbens in the exploratory motivation mechanisms and suggest that histamine might be an endogenous regulator.
Assuntos
Comportamento Exploratório/efeitos dos fármacos , Antagonistas dos Receptores Histamínicos/farmacologia , Histamina/farmacologia , Núcleo Accumbens/efeitos dos fármacos , Animais , Nível de Alerta/efeitos dos fármacos , Mapeamento Encefálico , Medo/efeitos dos fármacos , Injeções , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Motivação , Pirilamina/farmacologia , Ranitidina/farmacologia , Ratos , Ratos Sprague-Dawley , Tempo de Reação/efeitos dos fármacos , Meio SocialRESUMO
The possible role of histamine receptors in the hippocampal formation on the exploratory motivation and emotionality of the rat was studied. An elevated asymmetric plus-maze composed of 4 different arms (no walls, single high wall, high and low walls and two high walls) arranged at 90 degrees angles was used. The exploration score, considered to be an index of exploratory motivation, and the permanency score, considered to be an index of emotionality (anxiety), were determined. Histamine was administered locally into the ventral hippocampus at three different doses (9,45 and 90 nmol). Another group of rats was also microinjected with 45 nmol of pyrilamine (a histamine H1 receptor antagonist) or ranitidine (a histamine H2 receptor antagonist) in addition to 9 nmol of histamine in order to identify the possible type of histamine receptor involved. Histamine administration significantly inhibited the exploration score and increased the permanency score at the doses of 9 and 45 nmol in two of four arms. These effects were completely blocked by the administration of eitheer histamine receptor antagonist. The present results suggest that in the hippocampal formation histamine inhibits exploratory motivation and decreases emotionality by activating both types of histamine receptors. Also, the elvated asymmetric plus-maze appears to be a suitable technique to quantify exploration and possibly "anxiety".
Assuntos
Ratos , Animais , Masculino , Comportamento Exploratório/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Hipocampo/fisiologia , Histamina/farmacologia , Aprendizagem em Labirinto/efeitos dos fármacos , Pirilamina/farmacologia , Ranitidina/farmacologia , Receptores Histamínicos/fisiologia , Ratos Sprague-DawleyRESUMO
The possible role of histamine receptors in the hippocampal formation on the exploratory motivation and emotionality of the rat was studied. An elevated asymmetric plus-maze composed of 4 different arms (no walls, single high wall, high and low walls and two high walls) arranged at 90 degrees angles was used. The exploration score, considered to be an index of exploratory motivation, and the permanency score, considered to be an index of emotionality (anxiety), were determined. Histamine was administered locally into the ventral hippocampus at three different doses (9, 45 and 90 nmol). Another group of rats was also microinjected with 45 nmol of pyrilamine (a histamine H1 receptor antagonist) or ranitidine (a histamine H2 receptor antagonist) in addition to 9 nmol of histamine in order to identify the possible type of histamine receptor involved. Histamine administration significantly inhibited the exploration score and increased the permanency score at the doses of 9 and 45 nmol in two of four arms. These effects were completely blocked by the administration of either histamine receptor antagonist. The present results suggest that in the hippocampal formation histamine inhibits exploratory motivation and decreases emotionality by activating both types of histamine receptors. Also, the elevated asymmetric plus-maze appears to be a suitable technique to quantify exploration and possibly "anxiety".